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Critical Reviews™ in Eukaryotic Gene Expression
Facteur d'impact: 1.841 Facteur d'impact sur 5 ans: 1.927 SJR: 0.649 SNIP: 0.516 CiteScore™: 1.96

ISSN Imprimer: 1045-4403
ISSN En ligne: 2162-6502

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukarGeneExpr.v6.i2-3.20
pages 115-138

Scaffold/Matrix-Attached Regions: Topological Switches with Multiple Regulatory Functions

Jurgen Bode
GBF, National Center for Biotechnologioal Research, D-38124 Braunschweig, Mascheroder Weg 1
Michaela Stengert-lber
GBF, Gesellschaft fur Biotechnologische Forschung m.b.H., Genetik von Eukaryonten, D-38124 Braunschweig, Mascheroder Weg 1
Volker Kay
GBF, Gesellschaft fur Biotechnologische Forschung m.b.H., Genetik von Eukaryonten, D-38124 Braunschweig, Mascheroder Weg 1
Thomas Schlake
DKFZ, Abt. 0425 (Prof. Boehm), Raum 302, Im Neuheimer Feld 280, D-69120 Heidelberg.
Antje Dietz-Pfeilstetter
BBA, Biologische Bundesanstalt, Institut fur Biochemie und Pflanzenvirologie, D-38104 Braunschweig, Messeweg 11/12

RÉSUMÉ

The nuclear matrix or scaffold that can be prepared and investigated in vitro has an affinity for distinct topological forms of DNA and for sequences that permit the induction of such a topology by their association with a corresponding set of proteins (so-called matrix- or scaffold-attached regions, S/MARs). As a consequence, S/MARs are regarded as topological sinks that can be regulated according to activity-related requirements. Here we develop a switching model to understand the biological functions of S/MARs, which are able to augment transcriptional processes and form barriers between independently regulated domains. The current literature is screened for examples supporting such a mechanism and novel approaches are suggested for their further elucidation.