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Critical Reviews™ in Eukaryotic Gene Expression
Facteur d'impact: 1.841 Facteur d'impact sur 5 ans: 1.927 SJR: 0.649 SNIP: 0.516 CiteScore™: 1.96

ISSN Imprimer: 1045-4403
ISSN En ligne: 2162-6502

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukaryotGeneExpr.2019025602
pages 287-294

Molecular Docking Studies of Potential Inhibition of the Alcohol Dehydrogenase Enzyme by Phyllanthin, Hypophyllanthin and Gallic Acid

Subramanian Suganya
Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre
Lynn Schneider
Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre
Balaji Nandagopal
Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre

RÉSUMÉ

A review of literature shows certain phytochemicals, phyllanthin, hypophyllanthin and gallic acid have beneficial effects in experimental animals for improving liver metabolism in alcoholic liver disease. We investigated the ability of these chemicals to exhibit the inhibitory effect on the enzyme, alcohol dehydrogenase active site. The software used were CASTp, AutoDock and Molinspiration in Windows platform. We observed the phytochemicals, phyllanthin (−2.37 kcal/mol), hypophyllanthin (−3.23 kcal/mol) and gallic acid (−5.85 kcal/mol) in the order of increasing binding efficiency, which was as good as 4-methyl pyrazole (−4.18 kcal/mol).

RÉFÉRENCES

  1. Han K-H, Hashimoto N, Fukushima M. Relationships among alcoholic liver disease, antioxidants, and antioxidant enzymes. World J Gastroenterol. 2016;22:37-49.

  2. Chen X. Protective effects of quercetin on liver injury induced by ethanol. Pharmacogn Mag. 2010;6:135-141.

  3. Shankari SG, Karthikesan K, Jalaludeen AM, Ashokkumar N. Hepatoprotective effect of morin on ethanol-induced hepatotoxicity in rats. J Basic Clin Physiol Pharmacol. 2010;21:277-294.

  4. Li D, Ren D, Luo Y, Yang X. Protective effects of ursolic acid against hepatotoxicity and endothelial dysfunction in mice with chronic high choline diet consumption. Chem Biol Interact. 2016;258:102-107.

  5. Yao P, Li K, Song F, Zhou S, Sun X, Zhang X, Nussler AK, Liu, L. Heme oxygenase-1 upregulated by Ginkgo biloba extract: potential protection against ethanol-induced oxidative liver damage. Food Chem. Toxicol Int J Publ Br Ind Biol Res Assoc. 2007;45:1333-1342.

  6. Hanh ND, Sinchaipanid N, Mitrevej A. Physicochemical characterization of phyllanthin from Phyllanthus amarus Schum et Thonn. Drug Dev Ind Pharm. 2014;40:793- 802.

  7. Tripathi AK, Verma RK, Gupta AK, Gupta MM, Khanuja SPS. Quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by high-performance thin layer chromatography. Phytochem Anal PCA. 2006;17:394-397.

  8. Olayinka ET, Ore A, Ola OS, Adeyemo OA. Ameliorative effect of gallic acid on cyclophosphamide-induced oxidative injury and hepatic dysfunction in rats. Med Sci (Basel). 2015;3:78-92.

  9. McMartin KE, Collins TD. Distribution of oral 4-methylpyrazole in the rat: inhibition of elimination by ethanol. J Toxicol Clin Toxicol. 1988;26:451-66.

  10. Lee S-L, Lee Y-P, Wu M-L, Chi Y-C, Liu C-M, Lai C-L, Yin S-J. Inhibition of human alcohol and aldehyde dehydrogenases by aspirin and salicylate: assessment of the effects on first-pass metabolism of ethanol. Biochem Pharmacol. 2015;95:71-79.

  11. Haseba T, Sugimoto J, Sato S, Abe Y, Ohno Y. Phytophenols in whisky lower blood acetaldehyde level by depressing alcohol metabolism through inhibition of alcohol dehydrogenase 1 (class I) in mice. Metabolism. 2008;57:1753-1759.

  12. Kartkaya K, Oglakfi A, Senturk H, Bayramoglu G, Canbek M, Kanbak G. Investigation of the possible protective role of gallic acid on paraoxanase and arylesterase activities in livers of rats with acute alcohol intoxication. Cell Biochem Funct. 2013;31(3):208-13.

  13. Blomstrand R, Lof A, Ostling H. Studies on the metabolic effects of long-term administration of ethanol and 4-methylpyrazole in the rat. Vitamin A depletion in the liver. Nutr Metab. 1977;21(Suppl 1):148-151.

  14. Hurley TD, Bosron WF, Stone CL, Amzel LM. Structures of three human beta alcohol dehydrogenase variants. Correlations with their functional differences. J Mol Biol. 1994;239:415-429.

  15. Chirdchupunseree H, Pramyothin P. Protective activity of phyllanthin in ethanol-treated primary culture of rat hepatocytes. J Ethnopharmacol. 2010;128:172-176.

  16. Takahashi T, Sugawara W, Takiguchi Y, Takizawa K, Nakabayashi A, Nakamura M, Nagano-Ito M, Ichikawa S. Identification of plants that inhibit lipid droplet formation in liver cells: Rubus suavissimus leaf extract protects mice from high-fat diet-induced fatty liver by directly affecting liver cells. Evid-Based Comp Altern Med. 2016:4282758.