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Plasma Medicine
SJR: 0.271 SNIP: 0.316 CiteScore™: 1.9

ISSN Imprimer: 1947-5764
ISSN En ligne: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2014008858
pages 115-124

Effects of the Effluent of a Microscale Atmospheric Pressure Plasma-jet Operated with He/O2 Gas on Bovine Serum Albumin

Jan-Wilm Lackmann
Biology of Microorganisms, Faculty for Biology and Biotechnology, Ruhr University Bochum, Bochum, Germany
Eugen Edengeiser
Physical Chemistry II, Faculty for Chemistry and Biochemistry, Ruhr University Bochum, Bochum, Germany
Simon Schneider
Coupled Plasma-Solid State Systems, Faculty for Physics and Astronomy, Ruhr University Bochum, Bochum, Germany
Jan Benedikt
Coupled Plasma-Solid State Systems, Faculty for Physics and Astronomy, Ruhr University Bochum, Bochum, Germany
Martina Havenith
Physical Chemistry II, Faculty for Chemistry and Biochemistry, Ruhr University Bochum, Bochum, Germany
Julia E. Bandow
Biology of Microorganisms, Faculty for Biology and Biotechnology, Ruhr University Bochum, Bochum, Germany

RÉSUMÉ

Cold atmospheric pressure plasmas (CAPs) are being investigated for medical applications, and the first clinical studies are promising. However, interactions between plasmas and biological samples are only partly understood on a molecular level. In this study, bovine serum albumin (BSA), a standard model for plasma-mediated etching of biological samples, was used to investigate the effects of different components of an He/O2 plasma effluent on proteins. The X-jet features an optional lateral helium flow that splits the plasma effluent into particles and (V)UV radiation. BSA samples were exposed separately to plasma-emitted particles, UV radiation, or the combination of both. Afterward, plasma-treated samples were investigated using SDS-PAGE and western blot analysis for amino acid strand breaks, but none were detected. Furthermore, treated samples were investigated by Raman spectroscopy to search for chemical modifications. We found that treatment with the X-jet has little effect on BSA. Minor changes in the Raman spectra suggest modifications of tyrosine residues and some degree of oxidation of sulfur-containing amino acids. Our findings suggest that for the X-jet effluent, etching is the main effect of plasma on BSA, making BSA a suitable model with which to study protein etching.


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