Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
Plasma Medicine
SJR: 0.271 SNIP: 0.316 CiteScore™: 1.9

ISSN Imprimer: 1947-5764
ISSN En ligne: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2014008540
pages 125-136

Nonthermal Plasma Increases Expression of Wound Healing Related Genes in a Keratinocyte Cell Line

Annemarie Barton
Centre for Innovation Competence plasmatis, Greifswald, Germany; Leibniz Institute for Plasma Science and Technology (INP Greifswald), Greifswald, Germany
Kristian Wende
Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK Plasmatis, Greifswald, Germany
Lena Bundscherer
Center for Innovation Competence plasmatis, Greifswald, Germany; Leibniz Institute for Plasma Science and Technology, Greifswald, Germany
Sybille Hasse
Center for Innovation Competence plasmatis, Greifswald, Germany; Leibniz Institute for Plasma Science and Technology, Greifswald, Germany
Anke Schmidt
Centre for Innovation Competence plasmatis, Greifswald, Germany; Leibniz Institute for Plasma Science and Technology (INP Greifswald), Greifswald, Germany
Sander Bekeschus
Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK Plasmatis, Greifswald, Germany
Klaus-Dieter Weltmann
Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK Plasmatis, Greifswald, Germany
Ulrike Lindequist
Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald F.-L.-Jahn-Str. 17, D-17489 Greifswald, Germany
Kai Masur
Center for Innovation Competence plasmatis, Greifswald, Germany; Leibniz Institute for Plasma Science and Technology, Greifswald, Germany

RÉSUMÉ

The application of physical plasma in medicine has great potential in wound healing. Due to the generation of reactive oxygen and nitrogen species (ROS, RNS), emission of UV radiation and the generated electric fields can be used to stimulate epithelial and immune cells. To understand the processes on a molecular level the human keratinocyte cell line HaCaT was treated with a nonthermal atmospheric pressure argon plasma jet (kinpen). Subsequently, cellular RNA was isolated to conduct a quantitative polymerase chain reaction (qPCR) to monitor the magnitude of translation of genes related to wound healing. Plasma treatment induced an upregulation of vascular endothelial growth factor-a (VEGF-A), heparin-binding epidermal growth factor (EGF)-like growth factor (HBEGF), granulocyte macrophage colony-stimulating factor (GM-CSF), prostaglandin-endoperoxide synthase 2 (PTGS2) and interleukin-6 (IL-6) at the mRNA levels. This is a very promising result as the corresponding proteins are likely to be secreted and promote the wound healing process. Therefore plasma potentially induces the secretion of certain cytokines and growth factors, and hence, it could be the stimulus which is necessary to induce chronic wounds to heal.


Articles with similar content:

Role of Inflammation Amplifier-Induced Growth Factor Expression in the Development of Inflammatory Diseases
Critical Reviews™ in Immunology, Vol.35, 2015, issue 5
Yasunobu Arima, Daisuke Kamimura, Masaaki Murakami, Toru Atsumi, Ikuma Nakagawa
Lipocalin-2 in the Inflammatory Activation of Brain Astrocytes
Critical Reviews™ in Immunology, Vol.35, 2015, issue 1
Shinrye Lee, Mithilesh Kumar Jha, Kyoungho Suk
A Nitric Oxide Producing Pin-to-Hole Spark Discharge Plasma Enhances Endothelial Cell Proliferation and Migration
Plasma Medicine, Vol.1, 2011, issue 3-4
Krishna Priya Arjunan, Alisa Morss Clyne
Mechanisms of Wound Healing and Disinfection in a Plasma Source for the Treatment of Corneal Infections
Plasma Medicine, Vol.7, 2017, issue 2
L. Cordaro, Paola Brun, A. Pellizzaro, Roberto Cavazzana, Matteo Zuin, Emilio Martines
Radiofrequency Field-Induced Radiosensitization Is Related to Reductions in Metabolic Activity
Plasma Medicine, Vol.9, 2019, issue 2
Antonio Serafin, John Akudugu, Angela Chinhengo