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Plasma Medicine
SJR: 0.271 SNIP: 0.316 CiteScore™: 1.9

ISSN Imprimer: 1947-5764
ISSN En ligne: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2016015823
pages 47-57

Genotoxic and Cytotoxic Effects of Plasma-Activated Media on Multicellular Tumor Spheroids

Judee Florian
LAPLACE, Universite Paul Sabatier de Toulouse, Centre National de la Recherche Scientifique, Institut National Polytechnique de Toulouse, France
Nofel Merbahi
LAPLACE, Universite Paul Sabatier de Toulouse, Centre National de la Recherche Scientifique, Institut National Polytechnique de Toulouse, France
Mohammed Yousfi
LAPLACE, Universite Paul Sabatier de Toulouse, Centre National de la Recherche Scientifique, Institut National Polytechnique de Toulouse, 118 Route de Narbonne, Toulouse, France

RÉSUMÉ

The purpose of this study was to evaluate the effect of plasma-activated media (PAM) on a multicellular tumor spheroid (MCTS) obtained using HCT116 colon carcinoma cells. PAM was processed by a helium plasma jet during 60−240 seconds of exposure. Spheroids were immersed in PAM at different transfer times (from 15 minutes to 48 hours). The inhibitory effect of PAM on cell growth was first evaluated. This growth inhibition is associated with the regionalized accumulation of DNA damage detected by histone H2AX phosphorylation, leading to massive cell death and loss of the MCTS proliferative region. The use of a hydrogen peroxide (H2O2) scavenger (catalase) in PAM induced the reduction of DNA-damaged cells in MCTS, thus pointing out the role of H2O2 in DNA damage, but growth inhibition was still observed. Finally, whenHCT116 colorectal cancer cells and GM637 human fibroblast cells were immersed in PAM, the expression of γH2AX showed that only HCT116 cells were damaged, emphasizing an interesting selectivity of PAM to inactivate only the cancerous cells in the framework of in vitro cases; this selectivity has already been noted in the literature.


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