Publication de 4 numéros par an
ISSN Imprimer: 0731-8898
ISSN En ligne: 2162-6537
Indexed in
Radioprotective and Antioxidant Properties of Low-Altitude Podophyllum hexandrum (LAPH)
RÉSUMÉ
The development of nontoxic yet effective radioprotectors is needed because of the increasing risk of human exposure to ionizing radiation. We have reported that high-altitude Podophyllum hexandrum (HAPH) confers a radioprotective effect in in vitro and in vivo models. The present study reports on the antioxidant and radioprotective properties of low-altitude Podophyllum hexandrum (LAPH), from which the toxic compound podophyllotoxin has been partially removed during the extraction process. Using HPLC,we estimated the relative content of two marker compounds, podophyllotoxin and podophyllotoxin glycoside, in low-altitude Podophyllum extract (LAPE) and found them to be 23.3% and 9.50%, respectively. The ferrous ion chelation potential of LAPE was estimated using the 2,2 bipyridyl assay, and the activity was found to be increased concomitantly with the increase in its concentration, with a maximal inhibition at 25 μg/mL (42.20%) as compared to quercetin (34.9%). The electron donation potential of LAPE was also evaluated, because the antioxidant activities of natural products are known to bear a direct correlation with their ability to donate electrons. The concentration required to attain unit absorbance values at 700 nm were 0.230541±0.09 and 0.041±0.06 for butylated hydroxyl toluene and LAPE, respectively, indicating a higher antioxidant activity of LAPE. The free radical scavenging ability of LAPE was also assessed and exhibited a dose-dependant increase (1−100 μg/mL), comparable to that of quercetin at 25 μg/mL. The role of LAPE in protecting DNA was evaluated, and it was found that LAPE (30 μg/mL) rendered its maximum radioprotection against the 250 Gy-induced damage in the plasmid (pBR322) relaxation assay. LAPE significantly inhibited radiation-induced, iron/ascorbate- and combined stress (iron/ascorbate and radiation)-induced formation of TBARS (p<0.05). We conclude that LAPH, with its easy accessibility, ease of cultivation, multifarious radioprotective properties, and role as a renewable source of bioactive constituents, along with its low associated toxicity (due to partial removal of podophyllotoxin), enhances its possible use for human clinical applications.
-
Nejaim Yuri, I. V. Silva Amaro, V. Vasconcelos Taruska, J. N. L. Silva Emmanuel, M. de Almeida Solange, Evaluation of radioprotective effect of aloe vera and zinc/copper compounds against salivary dysfunction in irradiated rats, Journal of Oral Science, 56, 3, 2014. Crossref
-
Singh Manjinder, Kaur Maninder, Silakari Om, Flavones: An important scaffold for medicinal chemistry, European Journal of Medicinal Chemistry, 84, 2014. Crossref
-
Kumar Raj, Bansal Deen Dayal, Patel Dev Dutt, Mishra Saurabh, Karamalakova Yana, Zheleva A., Gadjeva Vessilina, Sharma Rakesh Kumar, Antioxidative and radioprotective activities of semiquinone glucoside derivative (SQGD) isolated from Bacillus sp. INM-1, Molecular and Cellular Biochemistry, 349, 1-2, 2011. Crossref
-
Benkovic V., Horvat Knezevic A., Dikic D., Lisicic D., Orsolic N., Basic I., Kosalec I., Kopjar N., Radioprotective effects of propolis and quercetin in γ-irradiated mice evaluated by the alkaline comet assay, Phytomedicine, 15, 10, 2008. Crossref
-
You Weir Chiang, Lin Wen Chuan, Huang Jia Tsz, Hsieh Chang Chi, Indigowood root extract protects hematopoietic cells, reduces tissue damage and modulates inflammatory cytokines after total-body irradiation: Does Indirubin play a role in radioprotection?, Phytomedicine, 16, 12, 2009. Crossref
-
Kma Lakhan, Plant Extracts and Plant-Derived Compounds: Promising Players in Countermeasure Strategy Against Radiological Exposure: A Review, Asian Pacific Journal of Cancer Prevention, 15, 6, 2014. Crossref
-
Singh Pankaj Kumar, Kumar Raj, Sharma Ashok, Arora Rajesh, Chawla Raman, Jain Swatantra Kumar, Tripathi Rajendra Prasad, Sharma Rakesh Kumar, Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines, Evidence-Based Complementary and Alternative Medicine, 2011, 2011. Crossref
-
Rao B. Nageshwar, Rao B.S. Satish, Aithal B. Kiran, Kumar M.R. Sunil, Radiomodifying and anticlastogenic effect of Zingerone on Swiss albino mice exposed to whole body gamma radiation, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 677, 1-2, 2009. Crossref
-
Kim Han Bit, Yoo Byung Sun, Propolis Inhibits UVA-Induced Apoptosis of Human Keratinocyte HaCaT Cells by Scavenging ROS, Toxicological Research, 32, 4, 2016. Crossref
-
Galhena Bandula Prasanna, Samarakoon S. S. R., Thabrew Myrtle Ira, Paul Solomon F. D., Perumal Venkatachalam, Mani Chinnadurai, Protective Effect of a Polyherbal Aqueous Extract Comprised ofNigella sativa(Seeds),Hemidesmus indicus(Roots), andSmilax glabra(Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes, BioMed Research International, 2017, 2017. Crossref
-
Rajesh Arora, Sagar R., Singh S., Kumar R., Sharma A. K., Prasad J., Singh S., Gupta M., Sharma R. K., Puri S. C., Krishna B., Siddiqui M. S., Lahiri S. S., Tripathi R. P., Qazi G. N., Cytoprotective Effect of Podophyllum hexandrum Against Gamma Radiation is Mediated via Hemopoietic System Stimulation and Up-Regulation of Heme-oxygenase-1 and the Prosurvival Multidomain Protein Bcl-2, Integrative Cancer Therapies, 6, 1, 2007. Crossref
-
Singh Pankaj Kumar, Kumar Raj, Sharma Ashok, Arora Rajesh, Chawla Raman, Jain Swatantra Kumar, Sharma Rakesh Kumar, Podophyllum hexandrum Fraction (REC-2006) Shows Higher Radioprotective Efficacy in the p53-Carrying Hepatoma Cell Line: A Role of Cell Cycle Regulatory Proteins, Integrative Cancer Therapies, 8, 3, 2009. Crossref
-
Arora Rajesh, DRDO Herbal Technologies: Military and Civil Applications, in New Age Herbals, 2018. Crossref
-
Singh Pankaj Kumar, Kumar Raj, Sharma Ashok, Arora Rajesh, Jain Swatantra Kumar, Sharma Rakesh Kumar, Pifithrin-α decreases the radioprotective efficacy of aPodophyllum hexandrum(Himalayan mayapple) fraction (REC-2006) in HepG2 cells, Biotechnology and Applied Biochemistry, 54, 1, 2009. Crossref
-
Puri Satish Chandra, Nazir Asiya, Chawla Raman, Arora Rajesh, Riyaz-ul-Hasan S., Amna Touseef, Ahmed Bilal, Verma Vijeshwar, Singh Shikha, Sagar Ravinder, Sharma Ashok, Kumar Raj, Sharma Rakesh Kumar, Qazi Ghulam Nabi, The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetralin lignans, Journal of Biotechnology, 122, 4, 2006. Crossref
-
Tsering Jambey, Tag Hui, Gogoi Baikuntha Jyoti, Vijay Veer , Traditional Anti-poison Plants Used by the Monpa Tribe of Arunachal Pradesh, in Herbal Insecticides, Repellents and Biomedicines: Effectiveness and Commercialization, 2016. Crossref
-
Ganie Showkat Ahmad, Haq Ehtishamul, Hamid Abid, Qurishi Yasrib, Mahmood Zahid, Zargar Bilal Ahmad, Masood Akbar, Zargar Mohmmad Afzal, Carbon tetrachloride induced kidney and lung tissue damages and antioxidant activities of the aqueous rhizome extract of Podophyllum hexandrum , BMC Complementary and Alternative Medicine, 11, 1, 2011. Crossref