Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
Journal of Environmental Pathology, Toxicology and Oncology
Facteur d'impact: 1.241 Facteur d'impact sur 5 ans: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimer: 0731-8898
ISSN En ligne: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2015013482
pages 183-189

Anticancer Property of Iron Oxide Nanoparticle-Drug Complexes: An In Vitro Study

S. Sreeja
MACFAST, Tiruvalla, Kerala, India
Cherupally Krishnan Krishnan Nair
Mar Athanasious College for Advanced Studies, Tiruvalla, Tiruvalla, Kerala, India; St.Gregorios Dental College & Research Centre, Kothamangalam, Kerala, India


Tumor-specific targeting of chemotherapeutic drugs can increase the therapeutic efficacy of most anticancer drugs. On surface-modified iron oxide nanoparticles (NPs), we complexed a hypoxic cell-targeting agent, sanazole (SAN), and a cytotoxic isoquinoline alkaloid, berberine (BBN). The major objective of this study was to elucidate the molecular mechanism of cytotoxicity in murine tumor cells (DLA) induced by NP−BBN-SAN complexes. The cytotoxicity of these complexes was determined using the dye exclusion method. The induction of apoptosis and cellular DNA damage in these cells was analyzed using dual staining and comet assay, respectively. The expression of genes in the treated cells elucidated the molecular mechanism underlying cytotoxicity. Cells treated with NP−BBN-SAN complexes showed significant increases in cytotoxicity and apoptosis, as well as extensive damage to cellular DNA compared to control cells. The cells treated with NP−BBN-SAN complexes showed greater DNA damage compared with other treatments. The increase in the expression of a pro-apoptotic gene suggested that apoptosis was the mechanism underlying cytotoxicity induced by NP−BBN-SAN complexes. Complexing with SAN increased the cytotoxic potential of NP−BBN complexes. Further in vivo studies are needed to evaluate the potential application of this method in controlling tumors.

Articles with similar content:

Biochanin A Enhances the Radiotoxicity in Colon Tumor Cells In Vitro
Journal of Environmental Pathology, Toxicology and Oncology, Vol.32, 2013, issue 3
Reeta Tiwari, Abhay Puthli, Kaushala Prasad Mishra
Antitumor and Hypoglycemic Activities of Polysaccharides from the Sclerotia and Mycelia of Inonotus obliquus (Pers.: Fr.) Pil. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.1, 1999, issue 4
Cun Zhuang, Kuniaki Abe, Tadashi Kiho, Takashi Mizuno, Hidehumi Okamoto, Sophie Leclerc, Shigeo Ukai, Laurent Meijer
DETANONOate Is a Potent Chemo\ Radio-Sensitizing Agent in Colon and Colorectal Cancers as Assessed in In Vitro and In Vivo Established Tumor Xenografts
Forum on Immunopathological Diseases and Therapeutics, Vol.1, 2010, issue 4
Xiaohuan Gao, Sergio Huerta, Benjamin Bonavida
Role of Hydroalcoholic Extract of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Facilitating Cellular Acclimatization in a Low-Oxygen Microenvironment
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 5
Kshipra Misra, Praveen Koganti, Manish Sharma, Rajkumar Tulsawani, Purva Sharma, Shivani Arora
Radioprotection against DNA Damage by an Extract of Indian Green mussel, Perna viridis (L)
Journal of Environmental Pathology, Toxicology and Oncology, Vol.26, 2007, issue 4
Parameswaran P. Subrayan, Anil Chatterji, Kaushala Prasad Mishra, Sreekumar P. Kumaran, Binoj C. Kutty