Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
Journal of Environmental Pathology, Toxicology and Oncology
Facteur d'impact: 1.241 Facteur d'impact sur 5 ans: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimer: 0731-8898
ISSN En ligne: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i4.30
pages 301-309

Effect of Amentoflavone, a Phenolic Component From Biophytum sensitivum, on Cell Cycling and Apoptosis of B16F-10 Melanoma Cells

K.S. Siveen
Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India
Girija Kuttan
Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala State, India

RÉSUMÉ

Flavanoids are polyphenolic compounds that are found in fruits and vegetables and have diverse, beneficial biochemical and antioxidant effects. The objective of this study was to assess the effect of amentoflavone, a biflavanoid isolated from Biophytum sensitivum, on cell cycling distribution and apoptosis in B16F-10 melanoma cells. Treatment of B16F-10 melanoma cells with amentoflavone (10 μg/mL) increased cells in the sub-G0/G1 phase accompanied by a decrease in G0/G1 phase cells in a time-dependent manner. A terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay of amentoflavone-treated B16F-10 melanoma cells confirmed that the cells were undergoing apoptosis. Amentoflavone was found to significantly inhibit B16F-10 melanoma-induced solid tumor development in C57BL/6 mice. The increase in apoptotic cells in paraffin sections obtained from amentoflavone-treated animals indicates that the reduction may be mediated through induction of apoptosis. Murine cell cycle-regulating genes, such as p21 and p27, and apoptosis-regulating genes, such as Bax and caspase-9, were found to be upregulated, whereas cyclin D1 and Bid were downregulated in amentoflavone-treated cells. These results demonstrate that amentoflavone can induce apoptosis via inhibiting progression of cells from G0/G1 to S phase and regulating genes involved in cell cycle regulation and apoptotic intrinsic pathways.


Articles with similar content:

Oleanolic acid induces Apoptosis by modulating p53, Bax, Bcl-2 and caspase-3 gene expression and regulates the activation of transcription factors and cytokine profile in B16F
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
P. Pratheeshkumar, Girija Kuttan
Perspectives on c-Myc, Cyclin D1, and Their Interaction in Cancer Formation, Progression, and Response to Chemotherapy
Critical Reviews™ in Oncogenesis, Vol.13, 2007, issue 2
Jack Wu, Fazlul H. Sarkar, D. Joshua Liao, Hector Biliran, Archana Thakur
Chemoprevention of Colon Cancer through Inhibition of Angiogenesis and Induction of Apoptosis by Nonsteroidal Anti-Inflammatory Drugs
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 3
Sankar Nath Sanyal, Preety Ghanghas, Chandan Rana, Shelly Jain
Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) and IL-23 Induced by Polysaccharide of the Black Hoof Medicinal Mushroom, Phellinus linteus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 3
Ha Won Kim, Soo Kyung Yoon, Dong Hee Lee, Soo Kyung Sung
Autophagy Inhibition Enhances SPCA-1 Cell Proliferation Inhibition Induced by By-1 from the Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 4
Di Wu, Wenbo Sun, Yanfang Liu, Yaping Zhang, Yan Yang, Li Min, Hairui Yang, Zheng Qiankun, Wei Jia, Wenhan Wang, Jinsong Zhang