RT Journal Article ID 65d1ad6a4de8a04c A1 Si, Jing A1 Cui, Bao-Kai T1 Study of the Physiological Characteristics of the Medicinal Mushroom Trametes pubescens (Higher Basidiomycetes) During the Laccase-Producing Process JF International Journal of Medicinal Mushrooms JO IJM YR 2013 FD 2013-03-22 VO 15 IS 2 SP 199 OP 210 K1 medicinal mushrooms K1 Trametes pubescens K1 physiological characteristic K1 laccase activity K1 antioxidative capability K1 metabolic pathway AB The study of the physiological characteristics of the medicinal mushroom Trametes pubescens was conducted under submerged cultures, suggesting that the laccase activity was positively correlated with oxidative level and culture conditions. Mycelial biomass and laccase activity in medium I were higher than those in medium II, which indicated that laccase activity was correlated with mycelium growth. The enhancement in mycelial biomass presented the logarithmic increase at days 6−8 and the peak value on the day 12 after inoculation. During liquid cultivation, increases in the amounts of malondialdehyde, hydrogen peroxide, and ascorbic acid were observed. In addition, the higher activities of superoxide dismutase and total antioxidative capacity still could be detected during this period. However, better ability to restrain hydroxyl free radical and catalase had a negative influence on laccase activity. It was evident that the fungal strain T. pubescens was under oxidative stress during the laccase-producing process. When the concentrations of H2O2 and Fe2+ were 3 and 30 mmol/L, respectively, the laccase activity reached to its peak at 37.21 U/L after a 14-day incubation period. It was concluded that a relationship between laccase synthesis and antioxidative capability existed in fungal cells, which could be regulated by reactive oxygen. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,72e9ed69099c0eef,65d1ad6a4de8a04c.html