RT Journal Article
ID 65d1ad6a4de8a04c
A1 Si, Jing
A1 Cui, Bao-Kai
T1 Study of the Physiological Characteristics of the Medicinal Mushroom Trametes pubescens (Higher Basidiomycetes) During the Laccase-Producing Process
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2013
FD 2013-03-22
VO 15
IS 2
SP 199
OP 210
K1 medicinal mushrooms
K1 Trametes pubescens
K1 physiological characteristic
K1 laccase activity
K1 antioxidative capability
K1 metabolic pathway
AB The study of the physiological characteristics of the medicinal mushroom Trametes pubescens was conducted under submerged cultures, suggesting that the laccase activity was positively correlated with oxidative level and culture conditions. Mycelial biomass and laccase activity in medium I were higher than those in medium II, which indicated that laccase activity was correlated with mycelium growth. The enhancement in mycelial biomass presented the logarithmic increase at days 6−8 and the peak value on the day 12 after inoculation. During liquid cultivation, increases in the amounts of malondialdehyde, hydrogen peroxide, and ascorbic acid were observed. In addition, the higher activities of superoxide dismutase and total antioxidative capacity still could be detected during this period. However, better ability to restrain hydroxyl free radical and catalase had a negative influence on laccase activity. It was evident that the fungal strain T. pubescens was under oxidative stress during the laccase-producing process. When the concentrations of H2O2 and Fe2+ were 3 and 30 mmol/L, respectively, the laccase activity reached to its peak at 37.21 U/L after a 14-day incubation period. It was concluded that a relationship between laccase synthesis and antioxidative capability existed in fungal cells, which could be regulated by reactive oxygen.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,72e9ed69099c0eef,65d1ad6a4de8a04c.html