RT Journal Article ID 5c50e40d27129bc9 A1 Leliebre-Lara, Vivian A1 Pferschy-Wenzig, Eva-Maria A1 Widowitz, Ute A1 Ortmann, Sabine A1 Lima, Clara Nogueiras A1 Bauer, Rudolf T1 Influence of Navisporus floccosus (Higher Basidiomycetes) n-Hexane Extract on Arachidonate Metabolism in Vitro JF International Journal of Medicinal Mushrooms JO IJM YR 2015 FD 2015-06-17 VO 17 IS 5 SP 463 OP 469 K1 medicinal mushrooms K1 Navisporus floccosus K1 anti-inflammatory K1 cyclooxygenase K1 leukotriene biosynthesis AB In vitro anti-inflammatory activity of 4 extracts with different polarity from the basidiomycete Navisporus floccosus was evaluated by determination of the inhibition of prostaglandin E2 formation catalyzed by purified cyclooxygenase (COX)-1 and COX-2 enzymes, and of the inhibition of leukotriene (LT) B4 formation in human polymorphonuclear leukocytes. The n-hexane extract showed the highest activity in all 3 assays. Through analysis by gas chromatography coupled with mass spectrometry (GC-MS), 9 fatty acids and fatty acid esters were identified as the major constituents of this extract. As several of them also showed inhibitory activity in the COX and LTB4 formation assays, it can be assumed that the unsaturated as well as the saturated fatty acids, and maybe also the fatty acid esters, present in the extract synergistically contribute to its in vitro anti-inflammatory activity. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,118fb2a34500f533,5c50e40d27129bc9.html