RT Journal Article
ID 513fd3d013bf2a46
A1 Ondrej, Vladan
A1 Havranek, Pavel
A1 Kitner, Miloslav
A1 Nemcova, Pavla
T1 Molecular Identification and Characterization of the Edible and Medicinal Morchellaceae Germplasm Collection of "Mulch Morels"
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2011
FD 2011-10-07
VO 13
IS 4
SP 369
OP 375
K1 morels
K1 edible and medicinal fungi
K1 ITS and AFLP analyses
K1 in vitro morel isolates
AB The accessions of the morel (Morchellaceae, Ascomycota) germplasm collection were genetically analyzed, in order to determine both their inter- and intraspecific relationships. This was done as a starting point for cultivation experiments, as well as to provide a genetic description of invasive morel populations linked to mulched garden patches, as compared with outdoor morels. The phylogenetic data, which was based on the internal transcribed spacer (ITS) sequences and supported by amplified fragment length polymorphism (AFLP) analyses, divided the germplasm isolates and accessions from the sequence database into three groups of yellow morels, and three groups of black morels, involving a remarkable monotypic genus of half-free morels (Mitrophora semilibera), the groups Morchella conica and M. angusticeps. Both Morchella groups include morel samples that use mulch bark as a vector for their spread across gardens in various locations in the Czech Republic. The AFLP analysis supported the ITS-based phylogenetic data and determined the intraspecific genetic profile of these, as a rule, almost entirely unstudied isolates.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7157a84928f91612,513fd3d013bf2a46.html