RT Journal Article
ID 3fa906e150ea8483
A1 Yang, Rui-Heng
A1 Wu, Yingying
A1 Tang, Li-Hua 
A1 Li, Chuan-Hua
A1 Shang, Jun-Jun
A1 Li, Yan 
A1 Song, Ying 
A1 Huang, Wei-Hua 
A1 Tao, Xiang-Sheng 
A1 Tan, Qi 
A1 Bao, Da-Peng
T1 The Reliability of DNA Sequences in Public Databases Belonging to the Most Economically Important Shiitake Culinary-Medicinal Mushroom <i>Lentinus edodes</i> (Agaricomycetes) in Asia
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2019
FD 2020-01-07
VO 21
IS 12
SP 1223
OP 1239
K1 <i>in silico</i>
K1 internal transcribed spacer
K1 <i>Lentinus edodes</i>
K1 medicinal mushrooms
K1 sequence reliability
AB Large numbers of DNA sequences deposited in the International Nucleotide Sequence Databases (INSD) are erroneously annotated. The erroneous information may lead to misleading conclusions or cause great economic losses to farmers. <i>Lentinus edodes (= Lentinula edodes</i> (Berk.) Pegler) is one of the most important and popular culinary-medicinal mushrooms with a high nutritional value. In this study, experimental and <i>in silico</i> methods were used to correct the sequences annotated as <i>L. edodes</i> in the INSD. A total of 3,426 nucleotide entries were retrieved from public databases, including 140 different types of genetic sequences. Excluding 1,893 genome sequences, the most abundant signatures represented by ITS (258) and IGS1 (259) sequences accounted for 33.23&#37; of the total entries. A total of 3,058 sequences were annotated correctly, 350 were indeterminate, and 18 were annotated erroneously based on the two methods. Correction of sequences will be beneficial for species identification and annotation. Phylogenic analysis based on ITS sequences suggested that <i>L. edodes</i> segregate in four clades in the tree based on ITS sequences. The isolates from China were distributed into two clades. In <i>L. edodes</i>, the intraspecific variation of the ITS2 sequences was much higher than that of the ITS1 sequences. In addition, the genetic diversity of the <i>L. edodes</i> sequences from China was much higher than that of any other regions included in this study. The northwest and southwest regions of China were <i>L. edodes</i> diversity centers.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,6f06ece20ea6c85f,3fa906e150ea8483.html