%0 Journal Article %A Villares, Ana %D 2013 %I Begell House %K medicinal and edible mushrooms, Lactarius deliciosus, red pine mushroom, polysaccharides, glucan, NMR. %N 6 %P 583-589 %R 10.1615/IntJMedMushr.v15.i6.60 %T Isolation and Characterization of a Glucan-Type Polysaccharide from the Red Pine Mushroom, Lactarius deliciosus (Higher Basidiomycetes) %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,034eeb045436a171,3cc4cd700e235bf7.html %V 15 %X A novel glucan-type polysaccharide has been isolated from the fruiting bodies of the edible mushroom Lactarius deliciosus. Two successive extractions (cold aqueous extraction at 25°C and hot aqueous extraction at 100°C) were performed, and the same polysaccharide was obtained in both fractions. The purity of the polysaccharide was evaluated by size exclusion chromatography. The SEC chromatogram showed a unique peak with a molecular weight of approximately 150 kDa. Analysis of the chain composition revealed that the polysaccharide was composed of glucose. Methylation analysis and NMR experiments showed that the glucan-type carbohydrate contained a main chain consisting of α-(1→3)-Glcp units with α-(1→4)-linked branches every sixth glucose residue. %8 2013-11-15