RT Journal Article
ID 17d31937050a7002
A1 Kumaran, Sekar
A1 Pandurangan, Ashok Kumar
A1 Shenbhagaraman, Ramalingam
A1 Esa, Norhaizan Mohd.
T1 Isolation and Characterization of Lectin from the Artist's Conk Medicinal Mushroom, Ganoderma applanatum (Agaricomycetes), and Evaluation of Its Antiproliferative Activity in HT-29 Colon Cancer Cells
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2017
FD 2017-10-16
VO 19
IS 8
SP 675
OP 684
K1 extracellular polysaccharides
K1 immunostimulation
K1 medicinal mushrooms
K1 NK cell activity
K1 phagocytic assay
K1 splenocyte proliferation
AB The growth and lectin production of Ganoderma applanatum, a white rot fungus, was optimized in broth cultures. The fungus was found to have a higher growth rate and higher lectin activity when grown in a medium adjusted to pH 6.5 at 26°C under stationary conditions. Expression of lectin activity started in 5-day-old mycelial culture; maximum activity was expressed after the 15th day of incubation. Among the various carbon and nitrogen sources tested, the carbon source sucrose and the nitrogen source yeast extract support maximum growth and lectin production. Lectin from G. applanatum was purified by ammonium sulfate precipitation and ion exchange chromatography. The purified fraction revealed a single band with a molecular weight of 35.0 kDa. Moreover, carbohydrates such as mannitol, glucose, sucrose, maltose, mannose, galactose, sorbose, and fructose were found to inhibit the hemagglutinating activity of the lectin. The purified lectins from G. applanatum contain cytotoxic and proapoptotic activities against HT-29 colon adenocarcinoma cells.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,32c8651274405055,17d31937050a7002.html