RT Journal Article
ID 149cca01476b2c21
A1 Cabrera, Rosina
A1 López-Peña, Damian
A1 Asaff, Ali
A1 Esqueda, Martín
A1 Valenzuela-Soto, Elisa M.
T1 Bioavailability of Compounds Susceptible to Enzymatic Oxidation Enhances Growth of Shiitake Medicinal Mushroom (Lentinus edodes) in Solid-State Fermentation with Vineyard Prunings
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-04-23
VO 20
IS 3
SP 291
OP 303
K1 laccase
K1 Lentinus edodes
K1 lignin degradation
K1 manganese peroxidase
K1 medicinal mushrooms
K1 phenolic compounds
AB Grapes are widely produced in northwestern Mexico, generating many wood trimmings (vineyard prunings) that have no further local use. This makes vineyard prunings a very attractive alternative for the cultivation of white-rot medicinal mushrooms such as Lentinus edodes. This type of wood can also offer a model for the evaluation of oxidative enzyme production during the fermentation process. We tested the effect of wood from vineyard prunings on the vegetative growth of and production of ligninolytic enzymes in L. edodes in solid-state fermentation and with wheat straw as the control substrate. The specific growth rate of the fungus was 2-fold higher on vineyard pruning culture (μM = 0.95 day-1) than on wheat straw culture (μM = 0.47 day-1). Laccase-specific production was 4 times higher in the vineyard prunings culture than on wheat straw (0.34 and 0.08 mU · mg protein-1 · ppm CO2-1, respectively), and manganese peroxidase production was 3.7 times higher on wheat straw culture than on vineyard prunings (2.21 and 0.60 mU · mg protein-1 · ppm CO2-1, respectively). To explain accurately these differences in growth and ligninolytic enzyme activity, methanol extracts were obtained from each substrate and characterized. Resveratrol and catechins were the main compounds identified in vineyard prunings, whereas epigallocatechin was the only one detected in wheat straw. Compounds susceptible to enzymatic oxidation are more bioavailable in vineyard prunings than in wheat straw, and thus the highest L. edodes growth rate is associated with the presence of these compounds.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,08829abe31900427,149cca01476b2c21.html