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International Journal on Algae

Publicado 4 números por año

ISSN Imprimir: 1521-9429

ISSN En Línea: 1940-4328

SJR: 0.168 SNIP: 0.377 CiteScore™:: 0.6 H-Index: 11

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Variability of intracellular elemental concentrations in freshwater phytoplankton estimated using SEM XRMA in relation to the conditions of analysis

Volumen 2, Edición 1, 2000, pp. 22-30
DOI: 10.1615/InterJAlgae.v2.i1.30
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SINOPSIS

Variability of elemental concentrations in cells of Ceratium hirundinella and Anabaena sp. measured by SEM XRMA were studied in relation to the conditions of the analyses. A point mode of analyses proved to be very variable due to the high heterogeneity of bioobjects, while results obtained by a medium-size reduced raster gave the best estimate of the concentrations in question. Larger sizes of reduced raster led to an underestimation of the measured intracellular concentrations probably due to a relatively high share of a cell wall and picking up void space, while results gained with smaller sizes of a reduced raster were subject to a high variability characteristic of a point mode analysis. Application of a medium-sized reduced raster could be recommended for measurements of intracellular elemental concentrations of freshwater phytoplankton species.

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