Publicado 4 números por año
ISSN Imprimir: 1521-9429
ISSN En Línea: 1940-4328
Indexed in
Variability of intracellular elemental concentrations in freshwater phytoplankton estimated using SEM XRMA in relation to the conditions of analysis
SINOPSIS
Variability of elemental concentrations in cells of Ceratium hirundinella and Anabaena sp. measured by SEM XRMA were studied in relation to the conditions of the analyses. A point mode of analyses proved to be very variable due to the high heterogeneity of bioobjects, while results obtained by a medium-size reduced raster gave the best estimate of the concentrations in question. Larger sizes of reduced raster led to an underestimation of the measured intracellular concentrations probably due to a relatively high share of a cell wall and picking up void space, while results gained with smaller sizes of a reduced raster were subject to a high variability characteristic of a point mode analysis. Application of a medium-sized reduced raster could be recommended for measurements of intracellular elemental concentrations of freshwater phytoplankton species.