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International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019032886
pages 1193-1205

Identification and In Silico Analysis of Lectins in Gray Oyster Culinary-Medicinal Mushroom Pleurotus ostreatus (Agaricomycetes) Based on the Transcriptomes

Danyun Xu
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Jing Lu
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Yuanyuan Wang
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Aafaque Ahmed Keerio
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Liesheng Zheng
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China
Liguo Chen
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China
Aimin Ma
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China; Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China


Lectins, one of the most important bioactive compounds, are nonimmunoglobulin proteins that can bind carbohydrates specifically. However, few reports have been published on Pleurotus ostreatus lectin at the molecular level. Hence, in this study, seven lectins were identified based on transcriptomes in four developmental stages, i.e., mycelium, primordium, young fruiting body, and mature fruiting body. The expression profiles of the lectin genes were verified by quantitative real-time PCR. Lectin2-lectin6 had the highest expression in mycelium, while lectin1 was rich in mature fruiting body, and lectin7 was in primordium. We inferred that lectin2-lectin6 may take part in cell flocculation, lectin7 was the critical gene for primordium formation, and lectinl may be involved in fruiting body maturation, respectively. By in silico analysis, all lectins were divided into three distinct groups. Lectin1-Lectin5 were about 38.5-40.7 kDa as extracellular protein and belonged to the PCL-like lectins. Lectin6 (15.4 kDa) was predicted in nucleus and belonged to fungal fruit body lectins. Lectin7 (38.5 kDa) was a member of legume-like lectins and located in the plasma membrane. This study will help us understand how lectins mediate mushroom development.


  1. Khan F, Khan M. Fungal lectins: current molecular and biochemical perspectives. Int J Biol Chem. 2011;5:1-20.

  2. Lam SK, Ng TB. Lectins: production and practical applications. Appl Microbiol Biotechnol. 2011;89(1):45-55.

  3. Singh RS, Bhari R, Kaur HP. Mushroom lectins: current status and future perspectives. Crit Rev Biotechnol. 2010;30(2):99-126.

  4. Rana T, Bera AK, Bhattacharya D, Das S, Pan D, Das SK. Characterization of arsenic-induced cytotoxicity in liver with stress in erythrocytes and its reversibility with Pleurotus florida lectin. Toxicol Ind Health. 2015;31(2):108-22.

  5. Kumaran S, Pandurangan AK, Shenbhagaraman R, Esa NM. Isolation and characterization of lectin from the Artist's Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes), and evaluation of its antiproliferative activity in HT-29 colon cancer cells. Int J Med Mushrooms. 2017;19(8):675-84.

  6. Varrot A, Basheer SM, Imberty A. Fungal lectins: structure, function and potential applications. Curr Opin Struct Biol. 2013;23(5):678-85.

  7. Singh RS, Kaur HP, Singh J. Purification and characterization of a mucin specific mycelial lectin from Aspergillus gorakhpurensis: application for mitogenic and antimicrobial activity. PLoS One. 2014;9(10):e109265.

  8. Kaneko T, Oguri S, Kato SI, Nagata Y. Developmental appearance of lectin during fruit body formation in Pleurotus cornucopiae. J Gen Appl Microbiol. 1993;39:83-90.

  9. Oguri S, Ando A, Nagata Y. A novel developmental stage-specific lectin of the basidiomycete Pleurotus cornucopiae. J Bacteriol. 1996;178(19):5692-98.

  10. Boulianne RP, Liu Y, Aebi M, Lu BC, Kues U. Fruiting body development in Coprinus cinereus: regulated expression of two galectins secreted by a nonclassical pathway. Microbiology. 2000;146(8):1841-53.

  11. Lu YP, Chen RL, Long Y, Li X, Jiang YJ, Xie BG. A jacalin-related lectin regulated the formation of aerial mycelium and fruiting body in Flammulina velutipes. Int J Mol Sci. 2016;17(12):E1884.

  12. Vetchinkina EP, Sokolov OI, Nikitina VE. Intracellular lectins of Lentinus edodes at various developmental stages of the fungus. Microbiology. 2008;77(4):496-501.

  13. Sun H, Zhao CG, Tong X, Qi YP. A lectin with mycelia differentiation and antiphytovirus activities from the edible mushroom Agrocybe aegerita. J Biochem Mol Biol. 2003;36(2):214-22.

  14. Luan R, Liang Y, Chen Y, Liu H, Jiang S, Che T, Wong B, Sun H. Opposing developmental functions of Agrocybe aegerita galectin (AAL) during mycelia differentiation. Fungal Biol. 2010;114(8):599-608.

  15. Sanchez C. Cultivation of Pleurotus ostreatus and other edible mushrooms. Appl Microbiol Biotechnol. 2010;85(5):1321-37.

  16. Yang D, Liang J, Wang Y, Sun F, Tao H, Xu Q, Zhang L, Zhang Z, Ho CT, Wan X. Tea waste: an effective and economic substrate for oyster mushroom cultivation. J Sci Food Agric. 2016;96(2):680-4.

  17. Lebeque Y, Morris HJ, Beltran Y, Llaurado G, Gaime-Perraud I, Meneses M, Moukha S, Bermudez RC, Garcia N. Proximal composition, nutraceutical properties, and acute toxicity study of culinary-medicinal oyster mushroom powder, Pleurotus ostreatus (Agaricomycetes). Int J Med Mushrooms. 2018;20(12):1185-95.

  18. Conrad F, Rudiger, H. The lectin from Pleurotus ostreatus: purification, characterization and interaction with a phosphatase. Phytochemistry. 1994;36(2):277-83.

  19. Davitashvili E, Kapanadze E, Kachlishvili E, Metreveli E, Elisashvili V. Comparative study of the hemagglutinating activity of lectins isolated from different developmental stages of culinary-medicinal oyster mushroom, Pleurotus ostreatus (Jacq.: Fr.) Kumm. (Agaricomycetideae). Int J Med Mushrooms. 2010;12(1):43-50.

  20. Wang HX, Gao JQ, Ng TB. A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem Biophys Res Commun. 2000;275(3):810-16.

  21. Sarangi I, Ghosh D, Bhutia SK, Mallick SK, Maiti TK. Antitumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans. Int Immunopharmacol. 2006;6(8):1287-97.

  22. Yin CM, Chen YY, Lu J, Ma AM. Cloning and expression analysis of lectin genes from Pleurotus ostreatus. Acta Hortic Sin. 2017;44(1):131-38.

  23. Ma A, Shan L, Wang N, Zheng L, Chen L, Xie B. Characterization of a Pleurotus ostreatus fruiting body-specific hydrophobin gene, Po.hyd. J Basic Microbiol. 2007;47(4):317-24.

  24. Zhou Y, Chen L, Fan X, Bian Y. De novo assembly of Auricularia polytricha transcriptome using Illumina sequencing for gene discovery and SSR marker identification. PLoS One. 2014;9(3):e91740.

  25. Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008;5(7):621-8.

  26. Audic S, Claverie JM. The significance of digital gene expression profiles. Genome Res. 1997;7(10):986-95.

  27. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-AACt method. Methods. 2001;25(4):402-8.

  28. Yu GJ, Wang M, Huang J, Yin YL, Chen YJ, Jiang S, Jin YX, Lan XQ, Wong BH, Liang Y, Sun H. Deep insight into the Ganoderma lucidum by comprehensive analysis of its transcriptome. PLoS One. 2012;7(8):e44031.

  29. Wang M, Gu B, Huang J, Jiang S, Chen Y, Yin Y, Pan Y, Yu G, Li Y, Wong BH, Li Y, Liang Y, Sun H. Transcriptome and proteome exploration to provide a resource for the study of Agrocybe aegerita. PLoS One. 2013;8(2):e56686.

  30. Wang W, Liu F, Jiang Y, Wu G, Guo L, Chen R, Chen B, Lu Y, Dai Y, Xie B. The multigene family of fungal laccases and their expression in the white rot basidiomycete Flammulina velutipes. Gene. 2015;563(2):142-9.

  31. Zhang J, Ren A, Chen H, Zhao M, Shi L, Chen M, Wang H, Feng, Z. Transcriptome analysis and its application in identifying genes associated with fruiting body development in basidiomycete Hypsizygus marmoreus. PLoS One. 2015;10(4):e0123025.

  32. Kojima M, Kimura N, Miura R. Regulation of primary metabolic pathways in oyster mushroom mycelia induced by blue light stimulation: accumulation of shikimic acid. Sci Rep. 2015;5:8630.

  33. Castanera R, Lopez-Varas L, Borgognone A, LaButti K, Lapidus A, Schmutz J, Grimwood J, Perez G, Pisabarro AG, Grigoriev IV, Stajich JE, Ramnez L. Transposable elements versus the fungal genome: impact on whole-genome architecture and transcriptional profiles. PLoS Genet. 2016;12(6):e1006108.

  34. Hirabayashi J. Lectins: methods and protocols. New York: Humana Press; 2014.

  35. Gabius HJ, Andre S, Jimenez-Barbero J, Romero A, Solis D. From lectin structure to functional glycomics: principles of the sugar code. Trends Biochem Sci. 2011;36(6):298-313.

  36. Cui Z, Maruyama Y, Mikami B, Hashimoto W, Murata K. Crystal structure of glycoside hydrolase family 78 alpha-L-Rhamnosidase from Bacillus sp. GL1. J Mol Biol. 2007;374(2):384-98.

  37. Fujimoto Z, Jackson A, Michikawa M, Maehara T, Momma M, Henrissat BF, Gilbert HJ, Kaneko S. The structure of a Streptomyces avermitilis alpha-L-rhamnosidase reveals a novel carbohydrate-binding module CBM67 within the six-domain arrangement. J Biol Chem. 2013;288(17):12376-85.

  38. Carrizo ME, Capaldi S, Perduca M, Irazoqui FJ, Nores GA, Monaco HL. The antineoplastic lectin of the common edible mushroom (Agaricus bisporus) has two binding sites, each specific for a different configuration at a single epimeric hydroxyl. J Biol Chem. 2005;280(11):10614-23.

  39. Satoh T, Cowieson NP, Hakamata W, Ideo H, Fukushima K, Kurihara M, Kato R, Yamashita K, Wakatsuki S. Structural basis for recognition of high mannose type glycoproteins by mammalian transport lectin VIP36. J Biol Chem. 2007;282(38):28246-5.

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