Publicado 12 números por año
ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344
Indexed in
Photoperiodic Responses and Characterization of the Cmvvd Gene Encoding a Blue Light Photoreceptor from the Medicinal Caterpillar Fungus Cordyceps militaris (Ascomycetes)
SINOPSIS
Light is a necessary environmental factor for production of conidia and pigment, formation of stroma, and development of Cordyceps militaris, a well-known edible and medicinal mushroom. In this study, an obvious rhythm loop was observed in certain strains of C. militaris under conditions of alternating 12-hour intervals of dark and light. A possibly related gene, Cmvvd, the homologue of the blue-light photoreceptor of Neurospora crassa, was cloned from the genome of C. militaris. The protein CmVVD is predicted to be 203 amino acids in length and is characterized by the presence of a light, oxygen, or voltage domain. Analysis of the CmVVD sensor domain (light, oxygen, or voltage) suggested that it is a blue-light receptor. Cysteine 108 is essential for the in vivo function of VIVID (VVD) in N. crassa photoadaptation. However, proline is in this position instead in all of the tested CmVVD proteins, suggesting that CmVVD may have a different function or may function in ways different from VVD in N. crassa. Genetic variation analysis of CmVVD in 6 representative strains indicated that 3 informative sites exist. Cmvvd messenger RNA was able to be induced by light, and the expression level increased over 10 times after irradiation and was maintained at high levels in the nascent fruiting body. The light-induced expression of Cmvvd was abolished in Cmwc-1 mutants, suggesting that the expression of Cmvvd is dependent on the photoreceptor CmWC-1 or on a functional CmWC-1/WC-2 complex. This article will help to open the still-unexplored field of circadian rhythms for this fungus.
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Schmoll Monika, Light, stress, sex and carbon – The photoreceptor ENVOY as a central checkpoint in the physiology of Trichoderma reesei, Fungal Biology, 122, 6, 2018. Crossref
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Zhang Jiaojiao, Wang Fen, Yang Ying, Wang Ying, Dong Caihong, CmVVD is involved in fruiting body development and carotenoid production and the transcriptional linkage among three blue‐light receptors in edible fungusCordyceps militaris, Environmental Microbiology, 22, 1, 2020. Crossref
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Lou Hai-Wei, Zhao Yu, Tang Hong-Biao, Ye Zhi-Wei, Wei Tao, Lin Jun-Fang, Guo Li-Qiong, Transcriptome Analysis of Cordyceps militaris Reveals Genes Associated With Carotenoid Synthesis and Identification of the Function of the Cmtns Gene, Frontiers in Microbiology, 10, 2019. Crossref
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Lou Haiwei, Lin Junfang, Guo Liqiong, Wang Xinwei, Tian Shuangqi, Liu Chenxi, Zhao Yu, Zhao Renyong, Advances in research on Cordyceps militaris degeneration, Applied Microbiology and Biotechnology, 103, 19, 2019. Crossref
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Huang Zi-Yan, Feng Lei, Fu Ming-Jia, Zhang Dan-Dan, Differential ubiquitome analysis of Cordyceps militaris lysine-ubiquitinated proteins affected by blue light, Biologia, 77, 7, 2022. Crossref
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Zhang Yiming, Zhou Yi, Wu Yijing, Cai Junhuo, Zhang Yiqiang, Shen Jincai, Wu Shibin, Liu Wenjie, Cai Ming, Cheng Tangren, Zhang Qixiang, Balance of Carotenoid Synthesis and Degradation Contributes to the Color Difference between Chinese Narcissus and Its Yellow-Tepal Mutant, Horticulturae, 8, 8, 2022. Crossref