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International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v18.i8.30
pages 671-680

Characterization of Cross-Flow Ultrafiltration Fractions from Maitake Medicinal Mushroom, Grifoia frondosa (Agaricomycetes), Reveals Distinct Cytotoxicity in Tumor Cells

Miriam Sari
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany
Reinhard Hambitzer
Competence Center for Applied Mycology and Environmental Studies, Department of Food Science and Nutrition, Niederrhein University of Applied Sciences, Mönchengladbach, Germany
Jan I. Lelley
GAMU Ltd. Institute for Mushroom Research Hüttenallee 241, D-47800 Krefeld, Germany
Katharina Toepler
Bio-Pharmaceutical Chemistry & Molecular Pharmacology, Technische Hochschule Koeln, Kaiser-Wilhelm-Allee, Chempark, 51368 Leverkusen, Germany
Nicole Teusch
Bio-Pharmaceutical Chemistry & Molecular Pharmacology, Technische Hochschule Koeln, Kaiser-Wilhelm-Allee, Chempark, 51368 Leverkusen, Germany; Department of Biomedical Sciences, Institute of Health Research and Education, University of Osnabrück, Barbarastr. 22c/ Building 93, 49076 Osnabrück, Germany
Anna Nickisch-Hartfiel
Department of Chemistry, Niederrhein University of Applied Sciences, Krefeld, Germany

SINOPSIS

β-glucans from Basidiomycetes like Grifola frondosa (the maitake mushroom) are well known for their health benefits. Polysaccharide preparations from medicinal mushrooms such as G. frondosa have been successfully tested in a vast number of studies. Many mushroom extracts have been developed and today are merchandized for use medicinally and commercially. Studies could show that, in particular, chemical structural features such as the molecular size of β-glucans significantly influence their bioactivity. Thus it is highly important to explore the composition and structural properties of β-glucans extracted from medicinal mushrooms and their effects on human tumor cell viability. Our study focuses on the molecular weight cutoff distribution of β-glucans in hot water−based extracts from maitake mushrooms. Cross-flow ultrafiltration was applied to obtain 5 fractions of different molecular size. β-glucan content was quantified using an enzyme-based test kit, specialized to 1,3-1,6-β-glucans. Here we show that only small amounts of β-glucans with a high molecular weight (>100 kDa) could be detected from an aqueous extract of G. frondosa. The main compounds encompass substances with a low molecular weight (<5 kDa), composing about 35% of the whole extract. In addition, tumor cell viability studies demonstrate significant cytotoxic potential in 2 different solid cancer cell types for the fraction with a high molecular weight (>100 kDa) and for 1 fraction with a low molecular weight (5−10 kDa). In summary, our experiments prove that cross-flow ultrafiltration serves as a quick and easy method for dividing crude aqueous mushroom extracts into different molecular-weight fractions that inhibit tumor cell viability in vitro.


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