Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

Volumes:
Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019030357
pages 311-322

Optimization of the Process for Producing Biomass and Exopolysaccharide from the King Tuber Oyster Mushroom, Pleurotus tuber-regium (Agaricomycetes), for Biotechnological Applications

Comfort Olukemi Bamigboye
Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, Eastern Cape, South Africa; Microbiology Unit, Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Nigeria
Julius Kola Oloke
Microbiology Unit, Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Nigeria
Mike Burton
Department of Mathematics, Rhodes University, Grahamstown, Eastern Cape, South Africa
Joanna Felicity Dames
Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, Eastern Cape, South Africa
Agbaje Lateef
Microbiology Unit, Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Nigeria

SINOPSIS

The optimization of microbial growth for biotechnological purposes traditionally requires an approach that uses only one variable at a time, which has many drawbacks. This research used a completely randomized approach to optimize carbon and nitrogen nutrient requirements and growth factors (pH and temperature) for Pleurotus tuber-regium in order to optimally produce biomass and extracellular polysaccharide (EPS) in shake-flask cultures. An artificial neural network (ANN) module was used to simulate the fungus-growing process and hence determine optimal conditions. The experiments demonstrated the effectiveness of the EPS fraction from P. tuber-regium in preserving hepatic cells against paracetamol-induced damage. Totals of 0.699 g biomass and 0.291 g EPS per 100 mL medium were obtained, whereas the ANN predicted 0.750 g biomass and 0.300 g EPS per 100 mL medium, thereby achieving 93.20% predictability for biomass and 73.00% predictability for EPS. Conditions for optimal EPS and biomass production for P. tuber-regium were quite different. Rat hepatic cells that had been fortified with the EPS fraction from P. tuber-regium were effectively preserved against liver damage. By using a mathematical approach, this study established optimal fermentation conditions for mycelia biomass and EPS production by P. tuber-regium and the relevant biotechnological implications.

REFERENCIAS

  1. Lau BF, Abdullah N, Aminudin N, Lee HB, Yap KC, Sabaratnam V. , The potential of mycelium and culture broth of Lignosus rhinocerotis as substitutes for the naturally occurring sclerotium with regard to antioxidant capacity, cytotoxic effect, and low-molecular-weight chemical constituents. PLoS One. 2014;9(7):e102509.

  2. Mahapatra S, Banerjee D. , Fungal exopolysaccharide: production, composition and applications. Microbiol Insights. 2013;6:1–16.

  3. Ma XK, Zhang H, Peterson EC, Chen L. , Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies. Carbohydr Polym. 2014;107:214–20.

  4. Bamigboye CO, Oloke JK, Dames JF. , Biological activity of extracellular and intracellular polysaccharides from Pleurotus tuber-regium hybrid and mutant strains. J Food Nutr Res. 2016;4(7):422–8.

  5. Berovič M, Habijanič J, Zore I, Wraber B, Hodžar D, Boh B, Pohleven F. , Submerged cultivation of Ganoderma lucidum biomass and immunostimulatory effects of fungal polysaccharides. J Biotechnol. 2003;103(1):77–86.

  6. Habijanic J, Berovic M, Boh B, Wraber B, Petravic-Tominac V. , Production of biomass and polysaccharides of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (higher Basidiomycetes), by submerged cultivation. Int J Med Mushrooms. 2013;15(1):81–90.

  7. Shen JW, Shi CW, Xu CP. , Exopolysaccharides from Pleurotus pulmonarius: fermentation optimization, characterization and antioxidant activity. Food Technol Biotechnol. 2013;51(4):520–7.

  8. Sarasvathy S, Raman J, Vikineswary S, Heng CK, Kuppusamy UR. , Functional properties of partially characterized polysaccharide from the medicinal mushroom Ganoderma neo-japonicum (Agaricomycetes). Int J Med Mushrooms. 2017;19(9):849–60.

  9. Song J, Cui L, Ma X, Su Y, Huang Z, Wang M., Optimization the fermentation conditions of Marasmius androsaceus by desirability function method. In: Liu H, Song C, Ram A, eds. Advances in applied biology. Proceedings of the 3rd International Conference on Applied Biotechnology (ICAB2016); 2016.

  10. Adeeyo AO, Lateef A, Gueguim-Kana EB. , Optimization of the production of extracellular polysaccharide from the shiitake medicinal mushroom Lentinus edodes (Agaricomycetes) using mutation and a genetic algorithm–coupled artificial neural network (GA-ANN). Int J Med Mushrooms. 2016;18(7):571–82.

  11. Kuo CH, Liu TA, Chen JH, Chang CM, Shieh CJ., Response surface methodology and artificial neural network optimized synthesis of enzymatic 2-phenylethyl acetate in a solvent-free system. Biocatal Agric Biotechnol. 2014;3(3):1–6.

  12. Silva R, Ferreira S, Bonifacio MJ, Dias JM, Queiroz JA, Passarinha LA. , Optimization of fermentation conditions for the production of human soluble catechol-O-methyltransferase by Escherichia coli using artificial neural network. J Biotechnol. 2012;160(3–4):161–8.

  13. Hornik K, Stinchcombe M, White H. , Multilayer feedforward networks are universal approximators. Neural Netw. 1989;2(5):359–66.

  14. Sinha K, Chowdhury S, Saha PD, Datta S. , Modeling of microwave-assisted extraction of natural dye from seeds of Bixa orellana (Annatto) using response surface methodology (RSM) and artificial neural network (ANN). Ind Crops Prod. 2013;41:165–71.

  15. Srikanth S, Swathi M, Tejaswini M, Sharmila G, Muthukumaran C, Jaganathan MK, Tamilarasan K., Statistical optimization of molasses based exopolysaccharide and biomass production by Aureobasidium pullulans MTCC 2195. Biocatal Agric Biotechnol. 2014;3(3):7–12.

  16. Chenghua D, Xiangliang Y, Xiaoman G, Yan W, Jingyan Z, Huibi X. , A beta-D-glucan from the sclerotia of Pleurotus tuber-regium (Fr.) Sing. Carbohydr Res. 2000;328(4):629–33.

  17. Zhang M, Cheung PC, Zhang L. , Evaluation of mushroom dietary fiber (nonstarch polysaccharides) from sclerotia of Pleurotus tuber-regium (Fries) Singer as a potential antitumor agent. J Agric Food Chem. 2001;49(10):5059–62.

  18. Wu GH, Hu T, Li ZY, Huang ZL, Jiang JG. , In vitro antioxidant activities of the polysaccharides from Pleurotus tuber-regium (Fr.) Sing. Food Chem. 2014;148:351–6.

  19. Wu JZ, Cheung PC, Wong KH, Huang NL. , Studies on submerged fermentation of Pleurotus tuber-regium (Fr.) Singer. Part 2: effect of carbon-to-nitrogen ratio of the culture medium on the content and composition of the mycelial dietary fibre. Food Chem. 2004;85(1):101–5.

  20. Zhang BB, Cheung PC. , Use of stimulatory agents to enhance the production of bioactive exopolysaccharide from Pleurotus tuber-regium by submerged fermentation. J Agric Food Chem. 2011;59(4):1210–6.

  21. Oso BA. , Pleurotus tuber-regium from Nigeria. Mycologia. 1977;69:271–9.

  22. Fasidi IO, Olorunmaiye KS. , Studies on the requirements for vegetative growth of Pleurotus tuber-regium (Fr.) Singer, a Nigerian mushroom. Food Chem. 1994;50(4):397–401.

  23. Wu JZ, Cheung PC, Wong KH, Huang NL., Studies on submerged fermentation of Pleurotus tuber-regium (Fr.) Singer—Part 1: physical and chemical factors affecting the rate of mycelial growth and bioconversion efficiency. Food Chem. 2003;81(3):389–93.

  24. White TJ, Bruns T, Lee SJ, Taylor J., Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, and White TJ, eds. PCR protocols: a guide to methods and applications. San Diego (CA): Academic Press; 1990. p. 315–22.

  25. Baker FJ, Silverton RE. , Introduction to medical laboratory technology. 5th ed. London (UK): Butterworth-Heinemann; 2014.

  26. Manjunathan J, Kaviyarasan V. , Physicochemical studies on Lentinus tuberregium (Fr.) Sing., a Indian edible fungus. Int J Pharm Sci. 2011;3:60–3.

  27. Isikhuemhen OS, Nerud F, Vilgalys R. , Cultivation studies on wild and hybrid strains of Pleurotus tuberregium (Fr.) Sing. on wheat straw substrate. World J Microbiol Biotechnol. 2000;16(5):431–5.

  28. Desai KM, Vaidya BK, Singhal RS, Bhagwat SS. , Use of an artificial neural network in modeling yeast biomass and yield of β-glucan. Process Biochem. 2005;40(5):1617–26.

  29. Madhloom MH. , Modeling of copper removal from simulated wastewater by adsorption on to fungal biomass using artificial neural network. Global J Adv Pure Appl Sci. 2015;(5):35–44.

  30. Chiranjeevi PV, Pandian MR, Thadikamala S. , Integration of artificial neural network modeling and genetic algorithm approach for enrichment of laccase production in solid state fermentation by Pleurotus ostreatus. Bioresources. 2014;9(2): 2459–70.

  31. Sun Z, Tian Y, Jia M, Pang L, Deng P, Fan K, Liu X, Jia S, Jia L. , Extraction and in vitro antioxidant activity of exopolysaccharide by Pholiota adiposa SX-01. Afr J Microbiol Res. 2012;6(8):1869–76.

  32. Ahmad ANG, Dachyar A, Logachanthirika S. , Optimization of complex fermentation media for glucose oxidase production using statistical approach. Pak J Biol Sci. 2013;16(18):960–4.

  33. Kana EB, Oloke JK, Lateef A, Oyebanji A. , Comparative evaluation of artificial neural network coupled genetic algorithm and response surface methodology for modeling and optimization of citric acid production by Aspergillus niger mcbn297. Chem Eng. 2012;27.

  34. He ZG, Yang YP, Shan ZH, Hu YH, Zhong H. , Effect of pyrite, elemental sulfur and ferrous ions on EPS production by metal sulfide bioleaching microbes. Trans Nonferrous Metal Soc China. 2014;24(4):1171–8.

  35. Zoberi MH. , Some edible mushrooms from Nigeria. Nigerian Field. 1973;38:81–91.

  36. Akpaja EO, Isikhuemhen OS, Okhuoya JA. , Ethnomycology and usage of edible and medicinal mushrooms among the Igbo people of Nigeria. Int J Med Mushrooms. 2003;5(3):313–9.

  37. Zhang ME, Cheung PC, Zhang L, Chiu CM, Ooi VE. , Carboxymethylated β-glucans from mushroom sclerotium of Pleurotus tuber-regium as novel water-soluble anti-tumor agent. Carbohydr Polym. 2004;57(3):319–25.

  38. Wong KH, Cheung PCK. , Sclerotium of culinary-medicinal king tuber oyster mushroom Pleurotus tuber-regium (Fr.) Singer (Agaricomycetidea): cultivation, biochemical composition, and biopharmaceutical effects (review). Int J Med Mushrooms. 2008;10:303–13.

  39. Afieroho O, Lawson E, Olutayo L, Adedokun OM, Emenyonu N. , Antituberculosis and phytochemical investigation of the dichloromethane extract Pleurotus tuber-regium (Fries) Singer sclerotium. Int Res J Pharm. 2013;4:255–7.

  40. Maness L, Sneed N, Hardy B, Yu J, Ahmedna M, Goktepe I. , Anti-proliferative effect of Pleurotus tuberregium against colon and cervical cancer cells. J Med Plants Res. 2011;5(30):6650–5.

  41. Wasser SP. , Medicinal mushrooms in human clinical studies. Part I. Anticancer, oncoimmunological, and immunomodulatory activities: a review. Int J Med Mushrooms. 2017;19(4):279–318.

  42. Liu D, Sun Q, Xu J, Li N, Lin J, Chen S, Li F. , Purification, characterization, and bioactivities of a polysaccharide from mycelial fermentation of Bjerkandera fumosa. Carbohydr Polym. 2017;167:115–22.

  43. Wasser SP. , Medicinal mushroom science: history, current status, future trends, and unsolved problems. Int J Med Mushrooms. 2010;12(1):1–16.


Articles with similar content:

The Influence of Cultivation Conditions on Mycelial Growth and Exopolysaccharide Production of Culinary-Medicinal Mushroom Pleurotus citrinopileatus Singer (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 3
Chiu-Yeh Wu
Extracellular Polysaccharide Production by Culinary-Medicinal Shiitake Mushroom Lentinus edodes (Berk.) Singer and Pleurotus (Fr.) P. Karst. Species Depending on Carbon and Nitrogen Source
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 2
Kok- Kheng Tan, Eva Kachlishvili, David Chichua, Vladimir I. Elisashvili
Cultivation of Three Pleurotus (Jacq.: Fr.) P. Kumm. Species on Pasteurized Elephant Grass (Pennisetum purpureum) Substrate
International Journal of Medicinal Mushrooms, Vol.9, 2007, issue 3&4
Elisandra Minotto, Jose Soares do Nascimento, Eduardo Bernardi, Lorena Pastorini Donini
Effect of Cultivation Conditions on the Production of Mycelial Biomass and Exopolysaccharide by Submerged Culture of a Rooting Shank Medicinal Mushroom, Oudemansiella radicata (Relhan) Singer (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Jinn-Chyi Wang, Chiu-Yeh Wu, Yeu-Pyng Lin
Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 4
Jasmina Simonic, Jelena Vukojević, Ilija Brceski, Sonja Duletic-Lausevic, Mirjana Stajic, Jasmina Glamoclija