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International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019030329
pages 331-342

Comparison of Chemical Compositions, Bioactive Ingredients, and In Vitro Antitumor Activity of Four Products of Cordyceps (Ascomycetes) Strains from China

Jie Qiao
Department of Bioengineering, College of Life Sciences, Huaibei Normal University, Huaibei, Anhui, China
Yuying Shuai
College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
Xin Zeng
Department of Bioengineering, College of Life Sciences, Huaibei Normal University, Huaibei, Anhui, China
Dayong Xu
Department of Bioengineering, College of Life Sciences, Huaibei Normal University, Huaibei, Anhui, China
Shengqi Rao
School of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, China
Huawei Zeng
Department of Bioengineering, College of Life Sciences, Huaibei Normal University, Huaibei, Anhui, China
Feng Li
Department of Bioengineering, College of Life Sciences, Huaibei Normal University, Huaibei, Anhui, China

SINOPSIS

The chemical compositions, bioactive ingredients, and in vitro antitumor activity were analyzed using water extracts from the dried fermented mycelia of Cephalosporium sinensis (C1), Paecilomyces hepiali (C2), Cordyceps cicadae (C3), as well as the dried fruiting bodies of C. militaris (C4). Total amino acid content was highest in C3, compared with C1, C2, C4, and natural C. militaris (S1). The contents of Fe, Zn, Mn, and Ca as well as the Zn/Cu ratio in C1-C3 were obviously different than those of C4 and S1, whereas Na contents and K/Na ratios in C3, C4, and S1 were obviously different from those in C1 and C2. Except for C4, cordycepin was not detected in C1-C3 or S1. The crude polysaccharide contents in C4 and total triterpenoid content in C4 were relatively lower than that in C1-C3. Detailed analysis of the crude polysaccharide from C4 did not detect fucose, but it contained the highest amount of glucose, compared with C1-C3. Additionally, the antitumor activities of the respective water extracts were ranked in the order of C3 > C4 > C1 > C2. These results can help us better understand products of Cordyceps strains.

REFERENCIAS

  1. Wang JQ, Kan LJ, Nie SP, Chen HH, Cui SW, Phillips AO, Li YJ, Xie MY. , A comparison of chemical composition, bioactive components and antioxidant activity of natural and cultured Cordyceps sinensis. LWT Food Sci Technol. 2015;63(1):2–7.

  2. Wang ZM, Peng X, Lee KLD, Tang JC, Cheung PCK, Wu JY. , Structural characterisation and immunomodulatory property of an acidic polysaccharide from mycelial culture of Cordyceps sinensis fungus Cs-HK1. Food Chem. 2011;125(2):637–43.

  3. Yao XL, Meran S, Fang YP, Martin J, Midgley A, Pan MM, Liu BC, Cui ST, Phillips GO, Phillips AO. , Cordyceps sinensis: in vitro anti-fibrotic bioactivity of natural and cultured preparations. Food Hydrocolloid. 2014;35:444–52.

  4. Tadamasa H, Meiling Y. , The antagonistic effects of Cephalosporium sinensis on ouabain induced cardiotoxicity and oxygen free radicals in guinea-pigs. China Pharm. 1997;8:57–8. (In Chinese).

  5. Wu ZW, Zhang MX, Xie MH, Dai ZQ, Wang XQ, Hu B, Ye H, Zeng XX. , Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1. Carbohydr Polym. 2015;137:541–8.

  6. Wu ZW, Lu JW, Wang XQ, Hu B, Ye H, Fan JL, Abid M, Zeng XX. , Optimization for production of exopolysaccharides with antitumor in vitro from Paecilomyces hepiali. Carbohydr Polym. 2014;99:226–34.

  7. Xiang M, Tang J, Zou XL, Zhao ZY, Wang YY, Xie SN. , β cell protecting and immunomodulatory activities of Paecilomyces hepiali Chen mycelium in STZ induced T1DM mice. Am J Chinese Med. 2009;37:361–72.

  8. Ukai S, Kiho T, Hara C, Morita M, Goto A, Imaizumi N, Hasegawa Y., Polysaccharides in fungi. XIII. Antitumor activity of various polysaccharides isolated from Dictyophora indusiata, Ganoderma japonicum, Cordyceps cicadae, Auricularia auricula-judae, and Auricularia species. Chem Pharmaceut Bull. 1983;31(2):741–4.

  9. Sharma SK, Gautam N. , Chemical composition and antioxidant and antibacterial activities of cultured mycelia of four clavicipitaceous mushrooms (Ascomycetes) from the Indian Himalayas. Int J Med Mushrooms. 2017;19(1):45–54.

  10. Chou SM, Lai WJ, Hong TW, Lai JY, Tsai SH, Chen YH, Yu SH, Kao CH, Chu RC, Ding ST, Li TK, Shen TL. , Synergistic property of cordycepin in cultivated Cordyceps militaris-mediated apoptosis in human leukemia cells. Phytomed. 2014;21(12):1516–24.

  11. Kim YS, Kim EK, Tang Y, Hwang JW, Natarajan SB, Kim WS, Moon SH, Jeon BT, Park PJ. , Antioxidant and anticancer effects of extracts from fermented Haliotis discus hannai with Cordyceps militaris mycelia. Food Sci Biotechnol. 2016;25(6):1775–82.

  12. Huang SJ, Huang FK, Purwidyantri A, Rahmandita A, Tsai SY. , Effect of pulsed light irradiation on bioactive, nonvolatile components and antioxidant properties of caterpillar medicinal mushroom Cordyceps militaris (Ascomycetes). Int J Med Mushrooms. 2017;19(6):547–60.

  13. Yu SJ, Zhang Y, Li CR, Zhang Q, Ma ZY, Fan, MZ., Optimization of ultrasonic extraction of mycelial polysaccharides from Paecilomyces hepiali using response surface methodology and its antioxidant activity. Afr J Biotechnol. 2013;10(75):17241–50.

  14. Shashidhar MG, Giridhar P, Sankar KU, Manohar B. , Bioactive principles from Cordyceps sinensis: a potent food supplement— a review. J Funct Foods. 2013;5(3):1013–20.

  15. Gaier ED, Kleppinger A, Ralle M, Mains RE, Kenny AM, Eipper BA. , High serum Cu and Cu/Zn ratios correlate with impairments in bone density, physical performance and overall health in a population of elderly men with frailty characteristics. Exp Gerontol. 2012;47(7):491–6.

  16. Ha E, Zemel MB. , Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people (review). J Nutr Biochem. 2003;14(5):251–8.

  17. Li Y, Zou X, Lv J, Yang, L, Li H, Wang W. , Trace elements in fingernails of healthy Chinese centenarians. Biol Trace Elem Res. 2012;145(2):158–65.

  18. Hsu TH, Shiao LH, Hsieh C, Chang DM. , A comparison of the chemical composition and bioactive ingredients of the Chinese medicinal mushroom Dong Chong Xia Cao, its counterfeit and mimic, and fermented mycelium of Cordyceps sinensis. Food Chem. 2002;78(4):463–9.

  19. Li C, Li Z, Fan M, Cheng W, Long Y, Ding T, Ming L. , The composition of Hirsutella sinensis, anamorph of Cordyceps sinensis. J Food Compos Anal. 2006;19(8):800–5.

  20. Yu L, Zhao J, Li SP, Fan H, Hong M, Wang YT, Zhu Q, Quality evaluation of Cordyceps through simultaneous determination of eleven nucleosides and bases by RP‐HPLC J Sep Sci 2006;29(7):953–8.

  21. Yang ZX, Chen BA, Wang H, Xia GG, Cheng J, Pei XP, Wang Fei, Bao W. , Handy, rapid and multiplex detection of tumor markers based on encoded silica–hydrogel hybrid beads array chip. Biosensors Bioelectron. 2013;48:153–7.

  22. Xiao YC, Chi XF, Dong Q, Tan L, Hu FZ. , Qualitative and quantitative comparative analysis of fatty acids in Cordyceps sinensis from different regions of Yushu prefecture of Qinghai Province. Chinese Pharmace J. 2015;50(13):1098–103. (In Chinese).

  23. Tüzen M. , Determination of heavy metals in fish samples of the middle Black Sea (Turkey) by graphite furnace atomic absorption spectrometry. Food Chem. 2003;80(1):119–23.

  24. Dubois M, Gilles KA, Hamilton JK, Rebers P, Smith F. , Colorimetric method for determination of sugars and related substances. Analyt Chem. 1956;28(3):350–6.

  25. Tsujikura Y, Higuchi T, Miyamoto Y, Sato S. , Manufacture of ganoderic acid by fermentation of Ganoderma lucidum. Jpn Kokai Tokkyo Koho JP. 1992:4304890.

  26. Li R, Zhao Y, Jiang X. , Chemical composition of Hirsutella beakdumountainsis, a potential substitute for Cordyceps sinensis. Afr J Biotechnol. 2011;10(72):16286–94.

  27. GB HealthWatch. Omega-3: omega-6 balance. Available from: http://www.gbhealthwatch.com/Science-Omega3-Omega6.php. Accessed March 2015.

  28. Stanaćev V Ž, Milić D, Milošević N, Stanaćev V S, Pavlovski Z, Škrbić Z, Vukić-Vranješ M. , Effect of vegetable oils on productive performances and lipid fatty acid composition of chicken abdominal fat. Biotechnol Anim Husbandry. 2013;29(4):631–40.

  29. Aggett PJ. , Physiology and metabolism of essential trace elements: an outline. Clinics Endocrinol Metab. 1985;14(3):513–43.

  30. Fraga CG. , Relevance, essentiality and toxicity of trace elements in human health. Mol Aspects Med. 2005;26(4–5):235–44.

  31. Cook NR, Obarzanek E, Cutler JA, Buring JE, Rexrode KM, Kumanyika SK, Appel LJ, Whelton PK. , Joint effects of sodium and potassium intake on subsequent cardiovascular disease: the trials of hypertension prevention follow-up study. Arch Intern Med. 2009;169(1):32–40.

  32. Tsuboi A, Watanabe M, Kazumi T, Fukuo K. , Association of serum copper/zinc ratio with low-grade inflammation and low handgrip strength in elderly women. Biomed Res Trace Elem. 2013;24(3):163–9.

  33. Li SP, Li P, Lai CM, Gong YX, Kan KK, Dong TT, Tsim KW, Wang YT., Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography. J Chromat A. 2004;103(2):239–43.

  34. Benowitz LI, Goldberg DE, Irwin N. , Inosine stimulates axon growth in vitro and in the adult CNS. Prog Brain Res. 2002;137:389–99.

  35. Nakamura K, Yoshikawa N, Yamaguchi Y, Kagota S, Shinozuka K, Kunitomo M. , Antitumor effect of cordycepin (3′-deoxyadenosine) on mouse melanoma and lung carcinoma cells involve adenosine A3 receptor stimulation. Anticancer Res. 2006; 26:43–7.

  36. Ruan W, Wei Y, Popovich DG. , Distinct responses of cytotoxic Ganoderma lucidum triterpenoids in human carcinoma cells. Phytother Res. 2015;29(11):1744–52.


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