Publicado 12 números por año
ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344
Indexed in
Ganoderma lucidum (Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) Inhibits Proliferation of Human Peripheral Blood Lymphocytes by Blocking lnterleukin-2 Secretion
SINOPSIS
Ganoderma lucidum is a famous medicinal mushroom that has various immunomodulating activities such as inhibition of antibody production, immunosuppressive antiviral activity, and inhibition of histamine release. However, there are few reports on the direct immunomodulating effects of this mushroom on human peripheral blood immunocompetent cells. For elucidation of the immunosuppressive mechanism of this mushroom, a methanolic (GLA) extract of its basidiocarps was fractionated into six parts. When each of the six fractions was applied to the adherent and nonadherent cells of human peripheral blood mononuclear cells (PBMCs), proliferative arrest was observed and growth arrest was stronger in the nonadherent cells than in the adherent cells. As the chloroform fraction of the fungus (GLE) arrested most strongly, it was further purified by silica gel column chromatography into seven subfractions. Among them, GLE1 fraction, the highest Rf value fraction, inhibited cell proliferation in a concentration-dependent manner. Antiproliferative activity of the GLE1 fraction was also observed in human allogeneic mixed lymphocyte culture (MLC). When secreted interleukin-2 (IL-2) of the culture supernatant of the MLC was measured by enzyme-linked immunosorbent assay (ELISA), the GLE1 fraction was found to inhibit IL-2 secretion, which was comparable to the effect of the immunosuppresive agent cyclosporin A. These data indicate that antiproliferating activity of the GLE1 fraction blocked IL-2 secretion. The GLE1 fraction of G. lucidum therefore may have the potential to be developed into an immunomodulating agent.