Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
Critical Reviews™ in Eukaryotic Gene Expression
Factor de Impacto: 1.841 Factor de Impacto de 5 años: 1.927 SJR: 0.649 SNIP: 0.516 CiteScore™: 1.96

ISSN Imprimir: 1045-4403
ISSN En Línea: 2162-6502

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukaryotGeneExpr.2019028155
pages 123-126

Antioxidant Enzymes Expression of the Impaired Contralateral Testes in Unilaterally Cryptorchid Rats

Bao-Ping Zhu
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China
Liang Cai
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China


Objective: In this study, explored the pathologic mechanism of the contralateral testes impairment in unilaterally cryptorchid rats by investigating the gene expression level. Methods: Thirty male Sprague-Dawley rats were randomly and evenly divided into two groups: cryptorchid group and control group. Cryptorchidism was induced by surgical relocation. RT-PCR was then applied to examine the mRNA expression level of antioxidant enzymes in descended testes, including glutathione peroxidase (GSH-PX), copper/zinc-superoxide dismutase (Cu/Zn-SOD), and catalase (CAT). The concentration of malondialdehyde (MDA) was determined by spectrophotometry. In addition, germ-cell apoptosis was detected by a terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Results: Two weeks after the operation, the mRNA expression levels of GSH-PX and Cu/Zn-SOD in the cryptorchid group were significantly downregulated; the expression of MDA, as well as the number of apoptotic germ cells, significantly increased compared to the control group (p < 0.01). The mRNA expression of CAT did not show significant changes (p > 0.05). Conclusion: GSH-PX and SOD were downregulated in the testis contralateral to the undescended testis, leading to the accumulation of reactive oxygen species and germ-cell apoptosis. Our results may provide molecular explanations for the impairment of the descended testis in unilateral cryptorchidism.


  1. Hutson JM, Balic A, Nation T, Southwell B. , Cryptorchidism. Semin Pediatr Surg. 2010;19:215–24.

  2. Virtanen HE, Toppari J. , Cryptorchidism and fertility. Endocrinol Metab Clin North Am. 2015;44:751–60.

  3. Group UKTCS. Aetiology of testicular cancer: association with congenital abnormalities, age at puberty, infertility, and exercise. U K Test Cancer Study Group. BMJ. 1994;308:1393–99.

  4. Cortes D. , Cryptorchidism–aspects of pathogenesis, histology and treatment. Scand J Urol Nephrol Suppl. 1998;196:1–54.

  5. Bergh A, Soder O. , Studies of cryptorchidism in experimental animal models. Acta Paediatr. 2007;96:617–21.

  6. Rio DC, Ares M, Jr., Hannon GJ, Nilsen TW. , Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc. 2010;2010:pdb prot5439.

  7. Khoubnasabjafari M, Ansarin K, Jouyban A. , Reliability of malondialdehyde as a biomarker of oxidative stress in psychological disorders. Bioimpacts. 2015;5:123–27.

  8. Kyrylkova K, Kyryachenko S, Leid M, Kioussi C. , Detection of apoptosis by TUNEL assay. Method Mol Biol. 2012;887:41–47.

  9. Foresta C, Ferlin A, Moro E, Garolla A, Rossato M, Scandellari C. , Microdeletions of the Y chromosome in cryptorchidism and in idiopathic male infertility. Ann Ital Med Int. 1999;14:151–58.

  10. Lenzi A, Gandini L, Lombardo F, Cappa M, Nardini P, Ferro F, Borrelli P, Dondero F. , Antisperm antibodies in young boys. Andrologia. 1991;23:233–35.

  11. Pinart E, Sancho S, Briz MD, Bonet S, Garcia N, Badia E. , Ultrastructural study of the boar seminiferous epithelium: changes in cryptorchidism. J Morphol. 2000;244:190–202.

  12. Zhu XS, Zheng XM, Li SW, Zheng H, Hu LQ. , Investigation of the mechanism of genitofemoral nerve in the contralateral testes damage of unilateral cryptorchid rats. Zhonghua Nan Ke Xue. 2002;8:264–65.

  13. Peltola V, Huhtaniemi I, Ahotupa M. , Antioxidant enzyme activity in the maturing rat testis. J Androl. 1992;13:450–55.

  14. Zini A, Schlegel PN. , Expression of glutathione peroxidases in the adult male rat reproductive tract. Fertil Steril. 1997;68:689–95.

  15. Zini A, Schlegel PN. , Effect of hormonal manipulation on mRNA expression of antioxidant enzymes in the rat testis. J Urol. 2003;169:767–71.

  16. Jana K, Dutta A, Chakraborty P, Manna I, Firdaus SB, Bandyopadhyay D, Chattopadhyay R, Chakravarty B. , Alpha-lipoic acid and N-acetylcysteine protects intensive swimming exercise-mediated germ-cell depletion, pro-oxidant generation, and alteration of steroidogenesis in rat testis. Mol Reprod Dev. 2014;81:833–50.

  17. Treulen F, Uribe P, Boguen R, Villegas JV. , Mitochondrial permeability transition increases reactive oxygen species production and induces DNA fragmentation in human spermatozoa. Hum Reprod. 2015;30:767–76.

  18. Kaur S, Bansal MP. , Protective role of dietary-supplemented selenium and vitamin E in heat-induced apoptosis and oxidative stress in mice testes. Andrologia. 2015;47:1109–19.

  19. Evangelista-Vargas S, Santiani A. , Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa. Reprod Domest Anim. 2017;52:819–24.

Articles with similar content:

Protective Effect of Troxerutin on Nickel-Induced Testicular Toxicity in Wistar Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 2
Ramalingam Ramakrishnan, Abdulkadhar Mohamed Jalaludeen, Leelavinothan Pari, Perumal Elangovan
Reversal of Lead-Induced Toxicity Due to the Effect of Antioxidants
Journal of Environmental Pathology, Toxicology and Oncology, Vol.32, 2013, issue 2
Sadhana Shrivastava, Samta Sharma, Sangeeta Shukla
Naringenin Alleviates Cadmium-Induced Toxicity through the Abrogation of Oxidative Stress in Swiss Albino Mice
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 2
Ruma Das, Avratanu Das, Sanjib Bhattacharya, Amrita Roy, Pallab Kanti Haldar
Lead Acetate-Induced Hepatoxicity in Wistar Rats: Possible Protective Role of Combination Therapy
Journal of Environmental Pathology, Toxicology and Oncology, Vol.34, 2015, issue 1
Amita Jaswal, Sadhana Shrivastava, Samta Sharma, Suchita Raghuvanshi, Sangeeta Shukla
Chemopreventive Action of Diclofenac in Dimethybenzanthracene Induced Lung Cancer in Female Wistar Rat
Journal of Environmental Pathology, Toxicology and Oncology, Vol.29, 2010, issue 3
Sankar Nath Sanyal, Poonam Thakur