Publicado 4 números por año
ISSN Imprimir: 1050-6934
ISSN En Línea: 1940-4379
Indexed in
Hydrogel Encapsulation to Improve Cell Viability during Syringe Needle Flow
SINOPSIS
This work examines pluronic F-127 poloxamer for cell protection during injection through a syringe needle. Direct cell injection is a minimally invasive method for cell transplantation; however, it often results in poor cell viability. We proposed that encapsulating cells in this hydrogel would protect cells from detrimental mechanical forces during injection and increase cell viability. The hydrogel was tested at multiple weights and carbon nanobrush concentrations to determine how gel weight affects cell viability as well as to allow the gels to remain as electrically conductive scaffolds. This work assessed the ability of the hydrogel to prevent cell membrane bursting. We used D1 multipotent mouse bone marrow stromal precursor cells for this study. We found that the pressure drop increases with increasing weight of the gels. However, cell viability also increases as the weight of the gels increases. These results support the proposition that hydrogels can be used to protect cells during syringe needle injection. Since these hydrogels undergo a reverse phase transition, the gels can be used to transplant cells into the body in solution form through injection. The gels will then harden in situ to allow for cell proliferation and tissue regeneration at the desired site.
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Baldari Silvia, Di Rocco Giuliana, Piccoli Martina, Pozzobon Michela, Muraca Maurizio, Toietta Gabriele, Challenges and Strategies for Improving the Regenerative Effects of Mesenchymal Stromal Cell-Based Therapies, International Journal of Molecular Sciences, 18, 10, 2017. Crossref
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Ashammakhi Nureddin, Ahadian Samad, Darabi Mohammad Ali, El Tahchi Mario, Lee Junmin, Suthiwanich Kasinan, Sheikhi Amir, Dokmeci Mehmet R., Oklu Rahmi, Khademhosseini Ali, Minimally Invasive and Regenerative Therapeutics, Advanced Materials, 31, 1, 2019. Crossref
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Naqvi Syeda M, Gansau Jennifer, Buckley Conor T, Priming and cryopreservation of microencapsulated marrow stromal cells as a strategy for intervertebral disc regeneration, Biomedical Materials, 13, 3, 2018. Crossref
-
Petta D, Armiento A R, Grijpma D, Alini M, Eglin D, D’Este M, 3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking, Biofabrication, 10, 4, 2018. Crossref
-
Soliman Bram G., Lindberg Gabriella C. J., Jungst Tomasz, Hooper Gary J., Groll Jürgen, Woodfield Tim B. F., Lim Khoon S., Stepwise Control of Crosslinking in a One‐Pot System for Bioprinting of Low‐Density Bioinks, Advanced Healthcare Materials, 9, 15, 2020. Crossref
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Kandilogiannakis Leonidas, Filidou Eirini, Kolios George, Paspaliaris Vasilis, Ad-Dressing Stem Cells: Hydrogels for Encapsulation, Processes, 9, 1, 2020. Crossref
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Palomares Laura A., Ramírez Octavio T., Hydrodynamic Stress and Heterogeneities in Animal Cell Culture, in Comprehensive Biotechnology, 2019. Crossref
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Kuddannaya Shreyas, Zhu Wei, Chu Chengyan, Singh Anirudha, Walczak Piotr, Bulte Jeff W. M., In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation, ACS Applied Materials & Interfaces, 13, 20, 2021. Crossref
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Mahendrasingam Shanthini, Osborn Anya, Israr Muhammad Zubair, Riasat Kiran, Gater Rachel VA, Spano Andrea, Gogo-Fyneface Desire, Worrall Amy J, Furness David N, The effects of substrate composition and topography on the characteristics and growth of cell cultures of cochlear fibrocytes, Hearing Research, 415, 2022. Crossref
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Han Seungsu, Kim Chul Min, Jin Songwan, Kim Taig Young, Study of the process-induced cell damage in forced extrusion bioprinting, Biofabrication, 13, 3, 2021. Crossref
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Xiao Ping, Li Yaping, Wang Dangge, Amplifying antitumor T cell immunity with versatile drug delivery systems for personalized cancer immunotherapy, Medicine in Drug Discovery, 13, 2022. Crossref