Publicado 4 números por año
ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537
Indexed in
Diallyl Disulfide Induces Caspase-Dependent Apoptosis via Mitochondria-Mediated Intrinsic Pathway in B16F-10 Melanoma Cells by Up-Regulating P53, Caspase-3 and Down-Regulating Pro-Inflammatory Cytokines and Nuclear Factor- κβ-Mediated Bcl-2 Activation
SINOPSIS
Diallyl disulfide (DADS) is a major organo-sulfur compound derived from garlic (Allium sativum), which inhibits the proliferation of various types of cancer cells. In this study we investigated the effect of DADS on the induction of apoptosis, as well as its regulatory effect on the activation of transcription factors in B16F-10 melanoma cells. Treatment of B16F-10 cells with nontoxic concentrations of DADS resulted in the presence of apoptotic bodies and induced DNA fragmentation in a dose-dependent manner. Cell-cycle analysis revealed that the occurrence of the sub-G1 peak was significantly elevated in DADS-treated cells. DADS treatment also down-reguated Bcl-2 expression and up-regulated p53, caspase-9, and caspase-3 expression in B16F-10 melanoma cells. The study also reveals that DADS inhibited the activation and nuclear translocation of p65, p50, and c-Rel subunits of nuclear factor (NF)-ℵB and other transcription factors, such as c-fos, activated transcription factor-2, and cyclic adenosine monophosphate response element-binding protein, in B16F-10 melanoma cells The pro-inflammatory cytokine production and gene expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and granulocyte-macrophage colony-stimulating factor (GM-CSF) were down-regulated in DADS-treated cells compared with control B16F-10 metastatic melanoma cells. DADS induces caspase-dependent apoptosis through a mitochondria-mediated intrinsic pathway in B16F-10 melanoma cells by activating p53 and caspase-3 gene expression and suppressing pro-inflammatory cytokines and NF-ℵB-mediated Bcl-2 activation.
-
Altonsy Mohammed O., Habib Tito N., Andrews Simon C., Diallyl Disulfide-Induced Apoptosis in a Breast-Cancer Cell Line (MCF-7) May Be Caused by Inhibition of Histone Deacetylation, Nutrition and Cancer, 64, 8, 2012. Crossref
-
Yi Lan, Su Qi, Molecular mechanisms for the anti-cancer effects of diallyl disulfide, Food and Chemical Toxicology, 57, 2013. Crossref
-
Dasgupta Pritha, (Bandyopadhyay) Sumita Sengupta, Role of Di-allyl Disulfide, a Garlic Component in NF-κB Mediated Transient G2-M Phase Arrest and Apoptosis in Human Leukemic Cell-lines, Nutrition and Cancer, 65, 4, 2013. Crossref
-
Trio Phoebe Zapanta, You Sixiang, He Xi, He Jianhua, Sakao Kozue, Hou De-Xing, Chemopreventive functions and molecular mechanisms of garlic organosulfur compounds, Food & Function, 5, 5, 2014. Crossref
-
Wang Yingyu, Huang Miaoyi, Sun Ruilin, Pan Lei, Extraction, characterization of a Ginseng fruits polysaccharide and its immune modulating activities in rats with Lewis lung carcinoma, Carbohydrate Polymers, 127, 2015. Crossref
-
Tsai Chia-Wen, Chen Haw-Wen, Sheen Le-Yen, Lii Chong-Kuei, Garlic: Health benefits and actions, BioMedicine, 2, 1, 2012. Crossref
-
Erekat Nour S., Apoptotic Mediators are Upregulated in the Skeletal Muscle of Chronic/Progressive Mouse Model of Parkinson's Disease, The Anatomical Record, 298, 8, 2015. Crossref
-
De Gianni Elena, Fimognari Carmela, Anticancer Mechanism of Sulfur-Containing Compounds, in Mechanism of the Anticancer Effect of Phytochemicals, 37, 2015. Crossref
-
Di Cui-xia, Han Lu, Zhang Hong, Xu Shuai, Mao Ai-hong, Sun Chao, Liu Yang, Si Jing, Li Hong-yan, Zhou Xin, Liu Bing, Miao Guo-ying, Diallyl disulfide attenuated carbon ion irradiation-induced apoptosis in mouse testis through changing the ratio of Tap73/ΔNp73 via mitochondrial pathway, Scientific Reports, 5, 1, 2015. Crossref
-
Huang Jing, Yang Bing, Xiang Tingxiu, Peng Weiyan, Qiu Zhu, Wan Jingyuan, Zhang Li, Li Hongyuan, Li Hongzhong, Ren Guosheng, Diallyl disulfide inhibits growth and metastatic potential of human triple-negative breast cancer cells through inactivation of the β-catenin signaling pathway, Molecular Nutrition & Food Research, 59, 6, 2015. Crossref
-
Yin Xiaoran, Zhang Jun, Li Xiaoning, Liu Dong, Feng Cheng, Liang Rongrui, Zhuang Kun, Cai Chenlei, Xue Xinghuan, Jing Fuchun, Wang Xijing, Wang Jun, Liu Xinlian, Ma Hongbing, DADS Suppresses Human Esophageal Xenograft Tumors through RAF/MEK/ERK and Mitochondria-Dependent Pathways, International Journal of Molecular Sciences, 15, 7, 2014. Crossref
-
Akhtar Salman, Khan M. Kalim A., Arif Jamal M., Evaluation and Elucidation Studies of Natural Aglycones for Anticancer Potential using Apoptosis-Related Markers: An In silico Study, Interdisciplinary Sciences: Computational Life Sciences, 10, 2, 2018. Crossref
-
Feng Liang, Yuan Ling, Du Meng, Chen Yan, Zhang Ming-Hua, Gu Jun-Fei, He Jun-Jie, Wang Ying, Cao Wei, Anti-Lung Cancer Activity through Enhancement of Immunomodulation and Induction of Cell Apoptosis of Total Triterpenes Extracted from Ganoderma luncidum (Leyss. ex Fr.) Karst., Molecules, 18, 8, 2013. Crossref
-
Xiao Xiangsheng, Chen Bo, Liu Xiaoping, Liu Peng, Zheng Guopei, Ye Feng, Tang Hailin, Xie Xiaoming, Navarro Alfons, Diallyl Disulfide Suppresses SRC/Ras/ERK Signaling-Mediated Proliferation and Metastasis in Human Breast Cancer by Up-Regulating miR-34a, PLoS ONE, 9, 11, 2014. Crossref
-
Yagdi Esma, Cerella Claudia, Dicato Mario, Diederich Marc, Garlic-derived natural polysulfanes as hydrogen sulfide donors: Friend or foe?, Food and Chemical Toxicology, 95, 2016. Crossref
-
Sahin Kazim, Ali Shakir, Sahin Nurhan, Orhan Cemal, Kucuk Omer, Lycopene: Multitargeted Applications in Cancer Therapy, in Natural Products and Cancer Drug Discovery, 2017. Crossref
-
Feng Cheng, Luo Yumei, Nian Yuanyuan, Liu Dong, Yin Xiaoran, Wu Jing, Di Jia, Zhang Rong, Zhang Jun, Diallyl Disulfide Suppresses the Inflammation and Apoptosis Resistance Induced by DCA Through ROS and the NF-κB Signaling Pathway in Human Barrett’s Epithelial Cells, Inflammation, 40, 3, 2017. Crossref
-
Kuo Chen-Hsin, Leu Yann-Lii, Wang Tong-Hong, Tseng Wei-Che, Feng Chun-Hao, Wang Shu-Huei, Chen Chin-Chuan, A novel DNA repair inhibitor, diallyl disulfide (DADS), impairs DNA resection during DNA double-strand break repair by reducing Sae2 and Exo1 levels, DNA Repair, 82, 2019. Crossref
-
Malla Rama Rao, Cellular and Molecular Mechanisms of Garlic Compounds in Common GI Cancers, in Phytochemicals Targeting Tumor Microenvironment in Gastrointestinal Cancers, 2020. Crossref
-
Liu Ran , Yang Ye‑Ning , Yi Lan , Qing Jing , Li Qing‑Ye , Wang Wen‑Song , Wang Juan , Tang Yu‑Xian , Tan Hui , Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway, Oncology Letters, 2018. Crossref
-
Leurcharusmee Prangmalee, Sawaddiruk Passakorn, Chattipakorn Nipon, Chattipakorn Siriporn C., Possible roles of garlic and its bioactive components on mitochondrial function in physiological and pathological conditions, in Mitochondrial Physiology and Vegetal Molecules, 2021. Crossref
-
Lv Chenghao, Wang Caiqiong, Li Ping, Huang Yiwen, Lu Xiangyang, Shi Meng, Zeng Chaoxi, Qin Si, Song Deguang, Effect of Garlic Organic Sulfides on Gene Expression Profiling in HepG2 Cells and Its Biological Function Analysis by Ingenuity Pathway Analysis System and Bio-Plex-Based Assays, Mediators of Inflammation, 2021, 2021. Crossref
-
Wang Yongyong, Fan Xiaowu, Wu Xiaowei, Ganoderma lucidum polysaccharide (GLP) enhances antitumor immune response by regulating differentiation and inhibition of MDSCs via a CARD9-NF-κB-IDO pathway, Bioscience Reports, 40, 6, 2020. Crossref
-
Pan Zhaohai, Luo Yongchuan, Xia Yuan, Zhang Xin, Qin Yao, Liu Wenjing, Li Minjing, Liu Xiaona, Zheng Qiusheng, Li Defang, Cinobufagin induces cell cycle arrest at the S phase and promotes apoptosis in nasopharyngeal carcinoma cells, Biomedicine & Pharmacotherapy, 122, 2020. Crossref
-
Rauf Abdur, Abu-Izneid Tareq, Thiruvengadam Muthu, Imran Muhammad, Olatunde Ahmed, Shariati Mohammad Ali, Bawazeer Saud, Naz Saima, Shirooie Samira, Sanches-Silva Ana, Farooq Umar, Kazhybayeva Galiya, Garlic (Allium sativum L.): Its Chemistry, Nutritional Composition, Toxicity, and Anticancer Properties, Current Topics in Medicinal Chemistry, 22, 11, 2022. Crossref
-
Mohammadzadeh Aghileh, Gol Ali, Synergistic properties of garlic and Citrullus colocynthis on reproductive injury caused by diabetes in male rats: Structural and molecular evidence , Journal of Food Biochemistry, 46, 12, 2022. Crossref