Publicado 4 números por año
ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537
Indexed in
Comparative evaluation of the cytotoxic and apoptotic potential of Poecilocerus pictus and Calotropis gigantea
SINOPSIS
Calotropis gigantea, the giant milkweed, is used traditionally for treatment against cancer and in Ayurveda as an antihelminthic, anti-pyretic and an anti-malarial agent. Poecilocerus pictus, an orthopteran insect feeds on Calotropis gigantea and both are known to possess cardiac glycosides. The increasing reports on the specific cytotoxicity of cardiac glycosides on human tumor cell lines led us to attempt characterization and comparative evaluation of cardenolides in both the insect and plant extracts for their anti-tumor and apoptotic potential. Chemical characterization using HPTLC, UV and IR analyses confirmed the presence of cardiac glycosides but differences in the components of the insect extract were indicative of biotransformation. The cytotoxicity studies revealed a more potent trend for the insect extract as compared to the plant extract on A549 and COLO205 cells. There was a considerably lesser measure of toxicity on WI38 cells and peripheral blood lymphocytes, while B16F1 remained unaffected by both the extracts. DNA ploidy analysis on COLO205 indicated that both the extracts induced dose dependent apoptosis. Thus both the insect and plant extracts differentiate between human cancer cells and normal cells and exhibit species specificity. Further investigations are necessary to establish both the extracts as promising lead candidates for antineoplastic activity.
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