Publicado 4 números por año
ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537
Indexed in
Evaluation of Genotoxicity of Medicinal Plant Extracts by the Comet and VITOTOX® Tests
SINOPSIS
We report the results of our genotoxic evaluation of extracts from three medicinal plants Acacia nilotica, Juglans regia, and Terminalia chebula and the herbal drug Triphala employing the VITOTOX® and comet tests.These tests detect DNA damage in prokaryotic and eukary-otic test systems, respectively. In the VITOTOX®test, none of the extracts were identified as genotoxic. In the comet assay, extracts of Acacia nilotica showed statistically significant DNA damage only in a concentration of 2500 ppm (highest tested dose), whereas extracts from Juglans regia showed significant damage in concentrations above 250 ppm and more. Extracts from Terminalia chebula and Tripahala significantly increased DNA damage in a concentration above 500 ppm. This is not considered contradictory, because DNA damage in the alkaline comet assay may not be permanent and hence may not necessarily result in mutations. All the extracts were previously found in the Ames assay to have potent antimutagenic effects against the direct acting mutagens NPD, sodium azide, and the S9-dependent mutagen 2-AF. The results of the previous study using the Ames assay are in conformity with those of the VITOTOX®test. It was found that the extracts were safe in concentrations of up to 1000 μg/0.1 mL and 2500 μg/0.1 mL. A literature survey also showed that plant extracts can be mutagenic as well as antimutagenic depending on the test system used. This indicates that a battery of assays is needed before any conclusion can be reached.
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van den Bout-van den Beukel Carolien J. P., Hamza Omar J. M., Moshi Mainen J., Matee Mecky I. N., Mikx Frans, Burger David M., Koopmans Peter P., Verweij Paul E., Schoonen Willem G. E. J., van der Ven André J. A. M., Evaluation of Cytotoxic, Genotoxic and CYP450 Enzymatic Competition Effects of Tanzanian Plant Extracts Traditionally Used for Treatment of Fungal Infections, Basic & Clinical Pharmacology & Toxicology, 102, 6, 2008. Crossref
-
Kalaivani T., Mathew Lazar, Free radical scavenging activity from leaves of Acacia nilotica (L.) Wild. ex Delile, an Indian medicinal tree, Food and Chemical Toxicology, 48, 1, 2010. Crossref
-
Kalaivani T., Rajasekaran C., Suthindhiran K., Mathew Lazar, Free Radical Scavenging, Cytotoxic and Hemolytic Activities from Leaves ofAcacia nilotica(L.) Wild. ex. Delile subsp.indica(Benth.) Brenan, Evidence-Based Complementary and Alternative Medicine, 2011, 2011. Crossref
-
Singh Rajbir, Singh Sukhpreet, Kumar Subodh, Arora Saroj, Free radical-scavenging activity of acetone extract/fractions of Acacia auriculiformis A. Cunn., Food Chemistry, 103, 4, 2007. Crossref
-
Sarwat Maryam, Das Sandip, Srivastava Prem S., Estimation of genetic diversity and evaluation of relatedness through molecular markers among medicinally important trees: Terminalia arjuna, T. chebula and T. bellerica, Molecular Biology Reports, 38, 8, 2011. Crossref
-
Singh Rajbir, Singh Sukhpreet, Kumar Subodh, Arora Saroj, Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn, Food and Chemical Toxicology, 45, 7, 2007. Crossref
-
Salem Manar M., Davidorf Frederick H., Abdel-Rahman Mohamed H., In vitro anti-uveal melanoma activity of phenolic compounds from the Egyptian medicinal plant Acacia nilotica, Fitoterapia, 82, 8, 2011. Crossref
-
Baliga M.S., Shivashankara A.R., Meera S., Palatty P.L., Haniadka R., Arora R., The Health Benefits of the Ayurvedic Anti-Aging Drugs (Rasayanas), in Bioactive Food as Dietary Interventions for the Aging Population, 2013. Crossref
-
Gardner Zoё, McGuffin Michael, Upton Roy, Bear Soaring, Winston David, Gagnon Daniel, Romm Aviva Jill, Dog Tieraona Low, Hardy Mary, Craker Lyle, Bechtel David, Beyer Leslie, Gurley Bill J., Schoenbart Bill, Parks Constance A., U, in American Herbal Products Association’s Botanical Safety, 2013. Crossref
-
Verschaeve Luc, High-Throughput Bacterial Mutagenicity Testing: Vitotox™ Assay, in High-Throughput Screening Methods in Toxicity Testing, 2013. Crossref
-
Bag Anwesa, Bhattacharyya Subir Kumar, Chattopadhyay Rabi Ranjan, The development of Terminalia chebula Retz. (Combretaceae) in clinical research, Asian Pacific Journal of Tropical Biomedicine, 3, 3, 2013. Crossref
-
Mattana C. M., Cangiano M. A., Alcaráz L. E., Sosa A., Escobar F., Sabini C., Sabini L., Laciar A. L., Evaluation of Cytotoxicity and Genotoxicity ofAcacia aromaLeaf Extracts, The Scientific World Journal, 2014, 2014. Crossref
-
Saleem Uzma, Mahmood Saeed, Ahmad Bashir, Saleem Mohammad, Anjum Aftab Ahmad, Estimation of genotoxic and mutagenic potential of latex and methanolic leaves extract of Euphorbia helioscopia by comet assay and Ames test, Asian Pacific Journal of Tropical Disease, 5, 2015. Crossref
-
Baliga Manjeshwar Shrinath, Meera Sharake, Shivashankara Arnadi Ramachandrayya, Palatty Princy Louis, Haniadka Raghavendra, The Health Benefits of Indian Traditional Ayurvedic Rasayana (Anti-aging) Drugs, in Foods and Dietary Supplements in the Prevention and Treatment of Disease in Older Adults, 2015. Crossref
-
Sharma Ashutosh, Flores-Vallejo Rosario del Carmen, Cardoso-Taketa Alexandre, Villarreal María Luisa, Antibacterial activities of medicinal plants used in Mexican traditional medicine, Journal of Ethnopharmacology, 208, 2017. Crossref
-
GUIDOTI D.G.G., GUIDOTI D.T., ROCHA C.L.M.S.C., MOURÃO K.S.M., Morphoanatomic characterization of the stem and the leaf of Tabernaemontana catharinensis A.DC (Apocynaceae) and antimutagenic activity of its leaves, Revista Brasileira de Plantas Medicinais, 17, 4 suppl 1, 2015. Crossref
-
Singh Prashant, Singh Pooja, Singh Mahesh Pratap, Assessment of Genetic Diversity and Evaluation of Relatedness Through Morphological and Molecular Markers Among Medicinally Important Trees: Terminalia arjuna, T. bellerica, T. catappa and T. chebula, National Academy Science Letters, 42, 2, 2019. Crossref
-
Mohamed Hanaa Mahmoud, Aly Magdy Sayed, Evaluation of genotoxicity of Euphorbia triaculeata Forssk. extract on mice bone marrow cells in vivo, Toxicology Reports, 5, 2018. Crossref
-
Gontijo Douglas Costa, Nunes Líria Granato, Farias Letícia Monteiro, Duarte Maria Gorette Resende, Carvalho André Furtado, Fietto Luciano Gomes, Leite João Paulo Viana, Assessment of the phenolic content, mutagenicity and genotoxicity of ethanolic extracts of stem bark and leaves from Strychnos pseudoquina A. St.-hil, Drug and Chemical Toxicology, 43, 5, 2020. Crossref
-
Pereira Erminiana Damiani de Mendonça, da Silva Juliana, Carvalho Patrícia da Silva, Grivicich Ivana, Picada Jaqueline Nascimento, Salgado Júnior Ilton Batista, Vasques Gabriela Jouglard, Pereira Mário Augusto da Silva, Reginatto Flavio Henrique, Ferraz Alexandre de Barros Falcão, In vivo and in vitro toxicological evaluations of aqueous extract from Cecropia pachystachya leaves, Journal of Toxicology and Environmental Health, Part A, 83, 19-20, 2020. Crossref
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Jităreanu Alexandra, Trifan Adriana, Vieriu Mădălina, Caba Ioana-Cezara, Mârțu Ioana, Agoroaei Luminița, Current Trends in Toxicity Assessment of Herbal Medicines: A Narrative Review, Processes, 11, 1, 2022. Crossref