Publicado 4 números por año
ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537
Indexed in
Effects of Shear Stress on Intracellular Calcium Change and Histamine Release in Rat Basophilic Leukemia (RBL-2H3) Cells
SINOPSIS
Massage, one form of physical therapy, is widely used for a large number of musculoskeletal disorders, but its exact mechanism still remains to be elucidated. One hypothesis is that the shear stress caused by massage may induce cutaneous mast cells to release histamine, thereby improving the local tissue microcirculation of blood. In the present work, a mast cell line (rat basophilic leukemia cells, RBL-2H3) was used in vitro to study cellular responses to the stimulus of shear stress generated by a rotating rotor in a cell dish. The intracellular calcium ([Ca2+]c) was studied by confocal fluorescence microscopy with Fluo-3/AM staining and the released histamine was measured with a fluorescence spectrometer using o-phthalaldehyde (OPA) staining. An elevation of [Ca2+]c occurred immediately after the shear stress, followed by histamine release. However, both [Ca2+]c increase and histamine release disappeared when a Ca2+-free saline was used, indicating that the rise in the [Ca2+]c is due to a Ca2+ influx from the extracellular buffer. Furthermore, Ruthenium red, a transient receptor potential vanilloid (TRPV) inhibitor, could effectively block the shear stressinduced histamine release, suggesting that TRPV membrane proteins are the likely targets of the shear stress. Because histamine is a well-known mediator of microvascular tissue dilation, these results may have an important impact on understanding the mechanism involved in massage therapy.
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Wei Fei, Shi Xiaomin, Chen Jiyao, Zhou Luwei, Fluid shear stress-induced cytosolic calcium signalling and degranulation dynamics in mast cells, Cell Biology International Reports, 19, 2, 2012. Crossref
-
Zhu Dan, Wu Zu-Hui, Chen Ji-Yao, Zhou Lu-Wei, The Effects of Electrical Stimuli on Calcium Change and Histamine Release in Rat Basophilic Leukemia Mast Cells, Chinese Physics Letters, 30, 6, 2013. Crossref
-
Shin Ein-Soon, Lee Sun-Hee, Seo Kyung-Hwa, Park Yoon-Hyung, Nguyen Tuyet T, Shin Ein-Soon, Aromatherapy and massage for symptom relief in patients with cancer, in Cochrane Database of Systematic Reviews, 2012. Crossref
-
Hu Kenneth K., Bruce Marc A., Butte Manish J., Spatiotemporally and mechanically controlled triggering of mast cells using atomic force microscopy, Immunologic Research, 58, 2-3, 2014. Crossref
-
Yao Wei, Huang Huaxiong, Ding Guanghong, A dynamic model of calcium signaling in mast cells and LTC4 release induced by mechanical stimuli, Chinese Science Bulletin, 59, 10, 2014. Crossref
-
Shin Ein-Soon, Lee Sun-Hee, Seo Kyung-Hwa, Shin Ein-Soon, Massage with or without aromatherapy for symptom relief in patients with cancer, in Cochrane Database of Systematic Reviews, 2014. Crossref
-
Kim Tae Joon, Nam Kung-Woo, Kim Bora, Lee Sung-Jin, Oh Ki-Bong, Kim Kyeong Ho, Mar Woongchon, Shin Jongheon, Inhibitory Effects of Costunolide Isolated from Laurus nobilis on IgE-induced Degranulation of Mast Cell-like RBL-2H3 Cells and the Growth of Y16 pro-B Cells, Phytotherapy Research, 2011. Crossref
-
Wu Zu-Hui, Zhu Dan, Chen Ji-Yao, Zhou Lu-Wei, The effects of thermal stimuli on intracellular calcium change and histamine releases in rat basophilic leukemia mast cells, Journal of Applied Physics, 111, 10, 2012. Crossref
-
Yao Wei, Yang Hongwei, Li Yabei, Ding Guanghong, Dynamics of Calcium Signal and Leukotriene C4Release in Mast Cells Network Induced by Mechanical Stimuli and Modulated by Interstitial Fluid Flow, Advances in Applied Mathematics and Mechanics, 8, 1, 2016. Crossref
-
Vysniauskaite Milda, Hertfelder Hans-Jörg, Oldenburg Johannes, Dreßen Peter, Brettner Stefan, Homann Jürgen, Molderings Gerhard J., Dileepan Kottarappat N, Determination of Plasma Heparin Level Improves Identification of Systemic Mast Cell Activation Disease, PLOS ONE, 10, 4, 2015. Crossref
-
Kim Jae Gon, Park Sang Woong, Byun Doyoung, Choi Wahn Soo, Sung Dong Jun, Shin Kyung Chul, Kim Hyun-ji, Leem Young-Eun, Kang Jong-Sun, Cho Hana, Kim Bokyung, Cho Sung I, Bae Young Min, Fluid flow facilitates inward rectifier K+ current by convectively restoring [K+] at the cell membrane surface, Scientific Reports, 6, 1, 2016. Crossref
-
Akyol Gokce Yilmaz, Manaenko Anatol, Akyol Onat, Solaroglu Ihsan, Ho Wing Mann, Ding Yan, Flores Jerry, Zhang John H., Tang Jiping, IVIG activates FcγRIIB-SHIP1-PIP3 Pathway to stabilize mast cells and suppress inflammation after ICH in mice, Scientific Reports, 7, 1, 2017. Crossref
-
Huang Meng, Wang Xuezhi, Xing Beibei, Yang Hongwei, Sa Zheyan, Zhang Di, Yao Wei, Yin Na, Xia Ying, Ding Guanghong, Critical roles of TRPV2 channels, histamine H1 and adenosine A1 receptors in the initiation of acupoint signals for acupuncture analgesia, Scientific Reports, 8, 1, 2018. Crossref
-
Suzuki Makoto, Mizuno Astuko, The Molecular Mechanism of Multifunctional Mechano-Gated Channel TRPV4, in Mechanically Gated Channels and their Regulation, 2012. Crossref
-
Kaouri K., Maini P. K., Skourides P. A., Christodoulou N., Chapman S. J., A simple mechanochemical model for calcium signalling in embryonic epithelial cells, Journal of Mathematical Biology, 78, 7, 2019. Crossref
-
Poerwosusanta Hery, Gunadi , Noor Zairin, Oktaviyanti Ika Kustiyah, Mintaroem Karyono, Pardjianto Bambang, Widodo Moch Aris, Widjajanto Edi, The effect of laparoscopy on mast cell degranulation and mesothelium thickness in rats, BMC Surgery, 20, 1, 2020. Crossref
-
Seidel Holger, Hertfelder Hans-Jörg, Oldenburg Johannes, Kruppenbacher Johannes P., Afrin Lawrence B., Molderings Gerhard J., Effects of Primary Mast Cell Disease on Hemostasis and Erythropoiesis, International Journal of Molecular Sciences, 22, 16, 2021. Crossref
-
Li Yingchen, Yu Yi, Liu Yuhang, Yao Wei, Mast Cells and Acupuncture Analgesia, Cells, 11, 5, 2022. Crossref
-
Wang Xuezhi, Yao Wei, Huang Meng, Zhang Di, Xia Ying, Ding Guanghong, Signal Transduction in Acupoints, in Advanced Acupuncture Research: From Bench to Bedside, 2022. Crossref
-
Yao Wei, Current Advances in Mathematical Models of Initial Response to Mechanical Stimulation at Acupoint, in Advanced Acupuncture Research: From Bench to Bedside, 2022. Crossref
-
Kaouri Katerina, Christodoulou Neophytos, Chakraborty Abhishek, Méndez Paul E., Skourides Paris, Ruiz-Baier Ricardo, A new mechanochemical model for apical constriction: Coupling calcium signalling and viscoelasticity, Frontiers in Systems Biology, 2, 2022. Crossref