RT Journal Article
ID 698ed0cd442bbeb1
A1 Cao, Peng-Fei
A1 Wu, Chen-Gao
A1 Dang, Zhi-Hao
A1 Shi, Liang
A1 Jiang, Ai-Liang
A1 Ren, Ang
A1 Zhao, Mingwen
T1 Effects of Exogenous Salicylic Acid on Ganoderic Acid Biosynthesis and the Expression of Key Genes in the Ganoderic Acid Biosynthesis Pathway in the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2017
FD 2017-03-06
VO 19
IS 1
SP 65
OP 73
K1 ganoderic acid
K1 Ganoderma lucidum
K1 gene expression
K1 medicinal mushrooms
K1 salicylic acid
AB We demonstrate herein that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in the lingzhi or reishi medicinal mushroom Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72% to 43.04% compared with the control group. When the fungi were treated with 200 μmol/L SA at different times, the GA content was improved 10.21% to 35.24% compared with the control group. By choosing the optimum point based on response surface methodology, the GA content could be increased up to 229.03 μg/100 mg, which was improved 66.38% compared with the control group. When the fungi were treated with 200 μmol/L SA, the transcription levels of key genes in the GA biosynthesis pathway−squalene (SQ) synthase (sqs), lanosterol (Lano; osc), and hydroxy-3-methylglutaryl-coenzyme A reductase (hmgr)−were improved 119.6-, 3.2-, and 4.2-fold, respectively. In addition, following treatment with 100 μmol/L SA, the levels of Lano and SQ, which are intermediate metabolites of GA biosynthesis, were increased 2.8- and 1.4-fold, respectively. These results indicate that SA can regulate the expression of genes related to GA biosynthesis and increases the metabolic levels of Lano and SQ, thereby resulting in the accumulation of GA.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7cc3420179d0cddd,698ed0cd442bbeb1.html