RT Journal Article ID 698ed0cd442bbeb1 A1 Cao, Peng-Fei A1 Wu, Chen-Gao A1 Dang, Zhi-Hao A1 Shi, Liang A1 Jiang, Ai-Liang A1 Ren, Ang A1 Zhao, Mingwen T1 Effects of Exogenous Salicylic Acid on Ganoderic Acid Biosynthesis and the Expression of Key Genes in the Ganoderic Acid Biosynthesis Pathway in the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2017 FD 2017-03-06 VO 19 IS 1 SP 65 OP 73 K1 ganoderic acid K1 Ganoderma lucidum K1 gene expression K1 medicinal mushrooms K1 salicylic acid AB We demonstrate herein that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in the lingzhi or reishi medicinal mushroom Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72% to 43.04% compared with the control group. When the fungi were treated with 200 μmol/L SA at different times, the GA content was improved 10.21% to 35.24% compared with the control group. By choosing the optimum point based on response surface methodology, the GA content could be increased up to 229.03 μg/100 mg, which was improved 66.38% compared with the control group. When the fungi were treated with 200 μmol/L SA, the transcription levels of key genes in the GA biosynthesis pathway−squalene (SQ) synthase (sqs), lanosterol (Lano; osc), and hydroxy-3-methylglutaryl-coenzyme A reductase (hmgr)−were improved 119.6-, 3.2-, and 4.2-fold, respectively. In addition, following treatment with 100 μmol/L SA, the levels of Lano and SQ, which are intermediate metabolites of GA biosynthesis, were increased 2.8- and 1.4-fold, respectively. These results indicate that SA can regulate the expression of genes related to GA biosynthesis and increases the metabolic levels of Lano and SQ, thereby resulting in the accumulation of GA. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7cc3420179d0cddd,698ed0cd442bbeb1.html