RT Journal Article
ID 4dae8b79365dda3b
A1 Sivanandhan, Subramaniyan
A1 Ganesan, Pathalam
A1 Paulraj, Michael Gabriel
A1 Ignacimuthu, Savarimuthu
T1 Larvicidal, Ovicidal, and Histopathological Effects of the Sulphur Polypore Mushroom, Laetiporus sulphureus (Agaricomycetes), Collected from Tamil Nadu, India against Mosquitoes
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-11-30
VO 20
IS 12
SP 1197
OP 1207
K1 Anopheles stephensi
K1 Culex quinquefasciatus
K1 histopathology
K1 Laetiporus sulphureus
K1 larvicidal activity
K1 medicinal mushroom
K1 ovicidal activity
K1 Poecilia reticulata
AB This study was conducted to determine the mosquitocidal activity of 6 species of higher Basidiomycetes collected from various places in the state of Tamil Nadu, India. The mushrooms were identified using MycoKey MMI software. We used morphological characteristics such as size, color, height, width (of the cap, lamellae, stipe, and fruiting body), spore deposits, and the presence or absence of ring to identify species. Eggs and larvae of Culex quinquefasciatus and Anopheles stephensi were exposed to various concentrations (62.5, 125.0, 250.0, and 500.0 ppm) of extracts from the 6 mushrooms. The extracts were dissolved in dimethyl sulfoxide. Among the 6 mushrooms, the methanol extract of Laetiporus sulphureus showed high toxicity toward mosquitoes at different life stages, with 96% larvicidal activity against A. stephensi and 76% activity against C. quinquefasciatus. After 24 hours, we found half-maximal lethal concentrations of 155.862 ppm for A. stephensi and 227.225 ppm for C. quinquefasciatus, and 90% lethal concentrations of 424.128 ppm for A. stephensi and 1011.663 ppm for C. quinquefasciatus. The extracts also showed strong ovicidal activity against mosquito eggs: 100% activity against A. stephensi and 91% activity against C. quinquefasciatus were observed at an extract concentration of 500 ppm 120 hours after treatment. The methanol extract of L. sulphureus was tested for toxicity against a nontarget organism, but we found none. Histopathological studies of the treated larvae revealed serious damage to cells in the midgut. These results suggest that the methanol extract of L. sulphureus is a good natural source for controlling mosquitoes.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2c6ab3993645ca9e,4dae8b79365dda3b.html