RT Journal Article ID 40d3c87e5493fa0d A1 Boel, Markus A1 Abilez, Oscar J. A1 Assar, Ahmed N A1 Zarins, Christopher K. A1 Kuhl, Ellen T1 IN VITRO/IN SILICO CHARACTERIZATION OF ACTIVE AND PASSIVE STRESSES IN CARDIAC MUSCLE JF International Journal for Multiscale Computational Engineering JO JMC YR 2012 FD 2012-03-13 VO 10 IS 2 SP 171 OP 188 K1 chain network models K1 multiscale modeling K1 cardiac muscle K1 electroactive materials K1 finiteelement method AB We propose a novel, robust, and easily reproducible, in vitro/in silico model system to characterize active and passive stresses in electroactive cardiac muscle using a hybrid experimental/computational approach. We explore active and passive stresses in healthy explanted heart slices in vitro, design a virtual test bed to simulate the in vitro measured stresses in silico, and predict altered active force generation in infarcted hearts in silico. For the in vitro model, explanted rat heart tissue slices are mounted on a force transducer and stimulated electrically through biphasic pulses. Isometric forces are recorded and translated into active circumferential stress. For the in silico model, stresses are additively decomposed into passive and active contributions, with the latter being related to the measured isometric force. A hierarchical finite element model for cardiac muscle tissue is developed based on passive tetrahedral unit cells, representing a network of interconnected polymeric chains, and active trusses, representing the contracting muscle fibers. First, we calibrate the model against our experiments with healthy explanted rat heart slices. Then, we predict acute and chronic alterations in active stress generation in infarcted hearts. We virtually explore isometric forces generation for different infarct area fractions and infarct locations. This approach has the potential to precisely quantify global loss of cardiac function for a given infarct area fraction. PB Begell House LK https://www.dl.begellhouse.com/journals/61fd1b191cf7e96f,42f1beba0b405e70,40d3c87e5493fa0d.html