A method of phycocyanin production from <i>Spirulina platensis</i> (Nordst.) Geitl.

V. F. Rudik; V.P. Bul'maga

doi:10.1615/InterJAlgae.v3.i1.70

International Journal on Algae

Chefredakteur: Solomon P. Wasser (open in a new tab)

Editor: Th. E. Smith (open in a new tab)

Redaktionelle Leiter: M.D. Aleinikova (open in a new tab)

Erscheint 4 Ausgaben pro Jahr

ISSN Druckformat: 1521-9429

ISSN Online: 1940-4328

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A method of phycocyanin production from Spirulina platensis (Nordst.) Geitl.

Volumen 3, Ausgabe 1, 2001, pp. 62-66

DOI: 10.1615/InterJAlgae.v3.i1.70

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V. F. Rudik
Moldova State University, Laboratory of Photomicrobiology, 65a, Kogelnichany St., and Institute of Microbiology and Biotechnology, National Academy of Sciences of Moldova Republic, 1, Akademicheskaya St., MD 2009 Kishinev, Moldova

V.P. Bul'maga
Moldova State University, Laboratory of Photomicrobiology, 65a, Kogelnichany St., 2009 Kishinev, Moldova

ABSTRAKT

Hydrophobia chromatography was used for the first time to purify phycobiliproteins. A method of phycocyanin production from the blue-green alga Spirulina platensis (Nordst.) Geitl. is proposed. The method includes isoelectric precipitation of proteins at pH Ј 4, production of the phycocyanin fraction I from the supernatant liquid, of the fraction II - from the sediment after its water-washing at pH < 4, and also subsequent purification by hydrophobic chromatography.

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