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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344
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Statistical Optimization of Polysaccharides Production by the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) in Solid-State Fermentation Using Highland Barley Grains
ABSTRAKT
Ganoderma lucidum is a widely used medicinal mushroom in traditional Chinese medicine that creates a diverse set of bioactive compounds. Highland barley, a typical nutrition-balanced crop, is not convenient for direct consumption but its nutritional characteristics meet the modern requirements of health food. In the present study, barley grains were used as substrates on solid-state fermentation (SSF) of G. lucidum. Statistical optimization of media composition was employed for enhancing the production of polysaccharides. Peptone, medlar, and KH2PO4 were identified as the most important components for producing polysaccharide. Based on the one-factor-at-a-time experimentation, a quadratic regression model with the polysaccharide contents as the response value was established by central composite design (CCD). The results showed that the predicted variables were 2.38% peptone, 1.14% medlar, and 0.25% KH2PO4 for the maximum yield of predicted polysaccharides of 11.64% in the fermented substrate. The experimental polysaccharides obtained using the predicted optimum media composition constituted 11.61% of the fermented substrate, which validates the high degree of accuracy of optimization for enhanced production of polysaccharides by SSF. This study suggested that in the process of barley grains fermentation by G. lucidum for polysaccharides production, the optimized SSF substrate consists of 71.4% barley grains, 2.38% peptone, 1.14% medlar, 0.25% KH2PO4, 2.5% glucose, 0.25% MgSO4 · 7H2O, and 1% CaCO3. According to this study, the strain G. lucidum 00679 showed a good fermentation property by optimizing the media. It might be a candidate industrial strain for further process optimization and scale-up study.
-
Zhou XW. Cultivation of Ganoderma lucidum: Edible and medicinal mushrooms. Technology and application. Hoboken, NJ: John Wiley & Sons; 2017. p. 385-413.
-
Soccol CR, Bissoqui LY, Rodrigues C, Rubel R, Sella RBR, Leifa F, De Souza Vandenberghe LP, Soccol VT. Pharmacological properties of biocompounds from spores of the lingzhi or reishi medicinal mushroom Ganoderma lucidum (Agaricomycetes): A review. Int J Med Mushrooms. 2016;18(9):757-67.
-
Ulziijargal E, Mau JL. Nutrient compositions of culinary-medicinal mushroom fruiting bodies and mycelia. Int J Med Mushrooms. 2011;13(4):343-9.
-
Paterson RRM. Ganoderma-a therapeutic fungal biofactory. Phytochemistry. 2006;67(18):1985-2001.
-
Zhou XW, Lin J, Yin YZ, Zhao JY, Sun XF, Tang KX. Ganodermataceae: Natural products and their related pharmacological functions. Am J Chin Med. 2007;35(4):559-74.
-
Li QZ, Wang XF, Zhou XW. Recent status and prospects of the fungal immunomodulatory protein family. Crit Rev Biotechnol. 2011;31(4):365-75.
-
Chen Y, Xie M, Zhang H, Wang YX, Nie SP, Li C. Quantification of total polysaccharides and triterpenoids in Ganoderma lucidum and Ganoderma atrum by near infrared spectroscopy and chemometrics. Food Chem. 2012;135(1):268-75.
-
Bishop KS, Kao CHJ, Xu YY, Glucina MP, Russell MR. From 2000 years of Ganoderma lucidum to recent developments in nutraceuticals. Phytochemistry. 2015;114:56-65.
-
Zhou XW, Su KQ, Zhang YM. Applied modern biotechnology for cultivation of Ganoderma and development of their products. Appl Microbiol Biotechnol. 2012;93(3):941-63.
-
Wang CH, Hsieh SC, Wang HJ, Chen ML, Lin BF, Chiang BH, Lu TJ. Concentration variation and molecular characteristics of soluble (1,3;1,6)-beta-D-glucans in submerged cultivation products of Ganoderma lucidum mycelium. J Agric Food Chem. 2014;62(3):634-41.
-
Li ZH, Zhou JL, Lin ZH. Development and innovation of Ganoderma industry and products in China. In: Lin ZB, Yang BX, editors. Advances in experimnetal medicine and biology. Springer; 2019. p. 187-204.
-
Habijanic J, Berovic M, Boh B, Wraber B, Petravic-Tominac V. Production of biomass and polysaccharides of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (W. Curt.:Fr.) P. Karst. (higher Basidiomycetes), by submerged cultivation. Int J Med Mushrooms. 2013;15(1):81-90.
-
Zapata PA, Rojas DF, Atehortua L. Production of biomass, polysaccharides, and ganoderic acid using non-conventional carbon sources under submerged culture of the lingzhi or reishi medicinal mushroom, Ganoderma lucidum (W. Curt.:Fr.) P. Karst. (higher Basidiomycetes). Int J Med Mushrooms. 2012;14(2):197-203.
-
Montoya S, Sanchez OJ, Levin L. Polysaccharide production by submerged and solid-state cultures from several medicinal higher Basidiomycetes. Int J Med Mushrooms. 2013;15(1):71-9.
-
Azizi M, Tavana M, Farsi M, Oroojalian F. Yield performance of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt.:Fr.) P. Karst. (higher Basidiomycetes), using different waste materials as substrates. Int J Med Mushrooms. 2012;14(5):521-7.
-
Erkel EI. The effect of different substrate mediums on yield of Ganoderma lucidum (Fr.) Karst. J Food Agric Environ. 2009;7(3-4):841-4.
-
Simonic J, Vukojevic J, Stajic M, Glamoclija J. Intraspecific diversity within Ganoderma lucidum in the production of laccase and Mn-oxidizing peroxidases during plant residues fermentation. Appl Biochem Biotechnol. 2010;162(2):408-15.
-
Li QZ, Chang YZ, Su KQ, Wang XL, Bai XH, Zhou XW. Cloning and characterization of the promoters of fungal immunomodulatory proteins gene from Ganoderma spp. and their response to methyl jasmonate and salicylic acid. Int J Med Mushrooms. 2018;20(2):177-89.
-
Cuesta G, Suarez N, Bessio M, Ferreira F, Massaldi H. Quantitative determination of pneumococcal capsular polysaccharide serotype 14 using a modification of phenol-sulfuric acid method. J Microbiol Methods. 2003;52(1):69-73.
-
Cai MH, Zhou XS, Sun XQ, Tao KJ, Zhang YX. Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus. J Ind Microbiol Biotechnol. 2009;36(3):381-9.
-
Box GEP, Hunter JS, Hunter WG. Statistics for experimenters: Design, innovation, and discovery. 2nd ed. Hoboken, NJ: John Wiley & Sons; 2005.
-
Song PR, Chang JD. Screening of Ganoderma lucidum strains with high extracellular polysaccharide productivity and the optimization of its submerged culture medium. Acta Edulis Fungi. 2013;10(2):9-16 (in Chinese).
-
Zhang W, Wang HX, Zhang JL, Zhang XY. Preliminary study on the fermentation of Lycium barbarum polysaccharides with Ganoderma lucidum to produce a polysaccharide composite. Sci Technol Food Ind. 2005;26(8):90-2 (in Chinese).
-
Han JR, An C, Yuan JM. Solid-state fermentation of cornmeal with the basidiomycete Ganoderma lucidum for degrading starch and upgrading nutritional value. J Appl Microbiol. 2005;99(4):910-5.
-
Han JR, Zhao WJ, Gao YY. A preliminary study on corn starch degradation by solid-state fermentation of Ganoderma lucidum. Food Ferment Ind. 2004;30(7):59-61 (in Chinese).
-
Gao WG, Shang N, Fan MT, Li PL. Optimization of solid-state fermentation medium of Ganoderma lucidum and nutrition component analysis. J China Agric Univ. 2011;16(1):88-93 (in Chinese).
-
Huang Jia-hua, Li Yong, Zhang Shan, Zou Yuan, Zheng Qian-wang, Lin Jun-fang, Guo Li-qiong, Amelioration effect of water extract from Ganoderma resinaceum FQ23 solid-state fermentation fungal substance with high-yield ergothioneine on anxiety-like insomnia mice, Food & Function, 13, 24, 2022. Crossref