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International Journal of Medicinal Mushrooms

Erscheint 12 Ausgaben pro Jahr

ISSN Druckformat: 1521-9437

ISSN Online: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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Partial Purification and Characterization of Polyphenoloxidase from Culinary-Medicinal Royal Sun Mushroom (the Himematsutake), Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae)

Volumen 13, Ausgabe 1, 2011, pp. 73-82
DOI: 10.1615/IntJMedMushr.v13.i1.90
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ABSTRAKT

The Royal Sun mushroom, the Himematsutake culinary-medicinal mushroom, Agaricus brasiliensis has several polyphenoloxidase activities in a broad sense. Here we report the partial purification of tyrosinase-type polyphenoloxidase (PPO). PPO is purified from A. brasiliensis without browning using a two-phase partitioning with Triton X-114 and ammonium sulfate fractionation. Partially denaturing SDS-PAGE (sodium dodecyl sulfate-polyacrylamide electrophoresis) staining with L-3,4-dihydroxyphenylalanine was performed and the indicated molecular sizes were approximately 70 kDa and 45 kDa. The purified enzyme is in its latent state and can be activated maximally in the presence of 1.6 mM sodium dodecyl sulfate (SDS). This enzyme catalyzes two distinct reactions, monophenolase and diphenolase activity, and the monophenolase activity showed a lag time typical of polyphenoloxidase. The Km value for 4-tert-butylcatechol was quite similar in the presence and absence of SDS, but the apparent Vmax value was increased 2.0-fold by SDS. Mimosine was a typical competitive inhibitor with Ki values of 138.2 μM and 281.0 μM n the presence and absence of SDS, respectively.

REFERENZIERT VON
  1. Yamanaka Daisuke, Tada Rui, Adachi Yoshiyuki, Ishibashi Ken-ichi, Motoi Masuro, Iwakura Yoichiro, Ohno Naohito, Agaricus brasiliensis-derived β-glucans exert immunoenhancing effects via a dectin-1-dependent pathway, International Immunopharmacology, 14, 3, 2012. Crossref

  2. Mathur Ritika, Navya P. N., Basavaraj K., Murthy Pushpa S., Bioprocess of robusta cherry coffee with polyphenol oxidase and quality enhancement, European Food Research and Technology, 240, 2, 2015. Crossref

  3. Yamanaka Daisuke, Tamiya Yumi, Motoi Masuro, Ishibashi Ken-ichi, Miura Noriko N., Adachi Yoshiyuki, Ohno Naohito, Heimesaat Markus M., The Effect of Enzymatically Polymerised Polyphenols on CD4 Binding and Cytokine Production in Murine Splenocytes, PLoS ONE, 7, 4, 2012. Crossref

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