Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i5.70
pages 457-465

A Validated Reverse-Phase HPLC Method for Quantitative Determination of Ganoderic Acids A and B in Cultivated Strains of Ganoderma spp. (Agaricomycetes) Indigenous to India

M. Ramakrishna
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India
D. Rajesh Babu
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India
S. S. Veena
Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, Karnataka, India
Meera Pandey
Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, Karnataka, India
Nageswara Rao
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India

ABSTRAKT

Twenty isolates of Ganoderma spp. indigenous to India were used in this study. A reverse-phase high-performance liquid chromatography (HPLC) method was used to quantify 2 of the most bioactive triterpenes, ganoderic acid A (GAA) and ganoderic acid B (GAB), among the cultivated Ganoderma spp. The HPLC analysis was performed on an Agilent 1260 Infinity HPLC system with a Zorbax C18 column, using gradient elution of acetonitrile and 0.1% acetic acid. The detection wavelength was set at 254 nm, with a flow rate of 0.6 mL/min. The method was validated according to the guidelines of International Conference on Harmonisation and produced satisfactory results. The amount of GAA varied from 827.50 to 2010.36 µg/g, whereas GAB varied from 16.64 to 916.89 µg/g. The developed method is simple, specific, accurate, and precise, and can be useful for qualitative and quantitative evaluation of ganoderic acids.


Articles with similar content:

Molecular Cloning and Characterization of a Subtilisin-Like Serine Protease Gene (Pr1) from the Medicinal Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 11
Zenghui Lu, Yuanchuan He, Xiaobing Zhang, Junhong Li, Jun Yan, Ping Shi, Shijiang Chen
Isolation and Characterization of Bioactive Metabolites from Fruiting Bodies and Mycelial Culture of Ganoderma oerstedii (Higher Basidiomycetes) from Mexico
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Jorge Suarez-Medellin, Cesar Espinoza, Manuel Norte, Angel Ramos-Ligonio, Jose J. Fernandez, Ángel Trigos, Guillermo Mendoza
Contents of Some Metabolites in the Peel and Flesh of the Medicinal Mushroom Wolfiporia cocos (F.A. Wolf) Ryvarden et Gilb. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 1
Yanli Zhao, Tao Li, Ji Zhang, Honggao Liu, Yuanzhong Wang
Mating System and Genetic Variations of Tricholoma crassum (Berk.) Sacc. in Some Area of Thailand by Isozyme Electrophoresis and PCR-RFLP Method
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Malinee Tantiyaporn, Aree Ritthiboon
Cultural and Metabolomic Studies of a New Phtalides Producer, Lignomyces vetlinianus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 11
Nadezhda V. Psurtseva, Katerina V. Sazanova, Alexey L. Shavarda