Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 22, 2020 Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v13.i4.90
pages 387-395

An Axenic Culture System for Fruiting Body Formation by an Edible Bolete Phylogenetically related to Culinary-Medicinal Penny Bun Mushroom, Boletus edulis Bull.:Fr. Strains from China

Shao Chun Fu
National Eng.Research Center of Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization, Chinese Ministry of Agriculture; Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi
Mei Yan Zhang
National Eng.Research Center of Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization, Chinese Ministry of Agriculture; Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi
Xiaodong Shang
National Eng.Research Center of Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization, Chinese Ministry of Agriculture; Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi
Ming-Jie Chen
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Agricultural Genetics and Breeding, Ministry of Agriculture, National Engineering Research Center for Edible Fungi, National R&D Center for Edible Fungi Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China
Qi Tan
National Engineering Research Center of Edible Fungi, Ministry of Science and Technology (MOST), Key Laboratory of Edible Fungal Resources and Utilization (South), Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

ABSTRAKT

The ability of two freshly isolated Boletus stains to fruit under axenic conditions was tested using different solid and liquid nutrient media. One strain (YNCX04) produced numerous primordia from which fruiting bodies, 12 mm and 10 mm in length, with grey, convex pilei, and yellow-white, clavate stipes developed between 15 and 30 d after inoculation of fungal mycelium onto a solid medium consisting of mineral salts, thiamine, glucose, potato, an extract of Cunninghamia lanceolata root, and agar. The other strain (YNB200) produced numerous primordia but no sporophores. Strain YNCX04 lost the ability to form fruiting bodies in axenic culture 6 mo after initial isolation but retained the ability to form primordia for up to 18 mo. Based on internal transcribed spacer sequencing data, strains YNB200 and YNCX04 formed a sub-cluster together with four previously designated Boletus edulis strains from China. Phylogenetic analysis placed the Chinese strains closer to B. aestivalis than to European and North American strains of B. edulis, although a 29-bp fragment specific to all the B. aestivalis strains was absent from all the Chinese strains. Furthermore, partial 18S rDNA sequences from strains YNB200 and YNCX04 exhibited 98% similarity with an 18S rDNA sequence from B. edulis strain Be3. Further molecular studies are indicated to more accurately establish the taxonomic positions of F3 and F4-3, as well as the Chinese strains designated as B. edulis.


Articles with similar content:

Cloning and Characterization of Laccase DNA from the Royal Sun Medicinal Mushroom, Agaricus brasiliensis (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 4
Akiko Matsumoto-Akanuma, Naohito Ohno, Akihiko Yamagishi, Satoshi Akanuma, Masuro Motoi
The Structure of the Polysaccharides Produced by Higher Basidiomyces Tremella mesenterica Ritz.: Fr. and Inonotus levis P. Karst.
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Bent O. Petersen, Jens O. Duus, Evgeny Vinogradov
Genomic Cloning and Characterization of a FIP-gsi Gene Encoding a Fungal Immunomodulatory Protein from Ganoderma sinense Zhao et al. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 1
Xuan-Wei Zhou, Fan Hong, Qi-zhang Li, Minqi Xie
Regulation of Growth and Biosynthetic Activity of the Medicinal Jelly Mushroom Tremella mesenterica (Retz.: Fr.) Pure Culture
International Journal of Medicinal Mushrooms, Vol.3, 2001, issue 1
Sergey V. Reshetnikov, Ina Duckman, Katherina Tsukor
Production of Biomass and Polysaccharides of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt. :Fr.) P. Karst. (Higher Basidiomycetes), by Submerged Cultivation
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 1
Vlatka Petravic-Tominac, Branka Wraber, Jozica Habijanic, Bojana Boh, Marin BEROVIC