Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 22, 2020 Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i1.20
pages 9-20

Cooperative Effect of the Lipopolysaccharide and Culinary-Medicinal Cauliflower Mushroom Sparassis crispa (Wulf.) Fr. (Aphyllophoromycetideae)-Derived β-Glucan on Inflammatory Cytokine Secretion by the Murine Macrophage Cell Line

Sung In Kim
Department of Life Sciences, University of Seoul, Seoul 130-743, Korea
Hyuk Gu Park
Hana Natural Substance Institute, Hanabiotech Ltd., Yeonchon, Gyonggi 486-860, Korea
Gye Hyung Cho
Department of Life Sciences, University of Seoul, Seoul 130-743, Korea
In Soo Ko
Hana Natural Substance Institute, Hanabiotech Ltd., Yeonchon, Gyonggi 486-860, Korea
Ha Won Kim
Department of Life Sciences, University of Seoul Dongdaemun-gu, Jennong-dong 90, Seoul 130-743, South Korea

ABSTRAKT

β-Glucans act as pathogen-associated molecular patterns (PAMPs), which can bind to the pattern recognition receptors (PRRs) on a macrophage or dendritic cell. Many kinds of mushroom β-glucans act as PAMPs and can bind to dectin-1 as one of the PRRs. High-molecular-weight β-glucans or intestinal normal microbiota can be transported through lumen epithelial cells, resulting in the stimulation of a macrophage in Peyer's patch. To study the involvement of TLR4 in the stimulation of dectin-1, the RAW264.7 macrophage cell line was stimulated using the β-glucan of Sparassis crispa (BGS) as well as the lipopolysaccharide (LPS) as a TLR4 ligand. The induction of IL-6 and the inducible nitric oxide synthase (iNOS) mRNAs was increased in a BGS and TNF-α concentration-dependent pattern. The increases of the TNF-α and IL-6 secretions after their treatment with 10 μg/mL BGS were found to be 204- and 420-fold, which are much higher, compared to those of the control. Moreover, the secretions of such cytokines were further increased by the presence of LPS, indicating the involvement of TLR4 in the signaling by dectin-1 with BGS. The secretion of nitric oxide (NO) was increased by BGS. These results show that BGS stimulated innate immunity by activating the macrophages through the induction of the dectin-1 receptor and the inflammatory cytokines. The secretion of inflammatory cytokines was influenced by TLR4 stimulation.


Articles with similar content:

Medicinal Mushroom Lingzhi or Reishi, Ganoderma lucidum (W.Curt.:Fr.) P. Karst., β-Glucan Induces Toll-like Receptors and Fails to Induce Inflammatory Cytokines in NF-κB Inhibitor-Treated Macrophages
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 3
Mi Jeong Kim, Ha Won Kim, Sainkhuu Batbayar
β-Glucan of Ganoderma lucidum (W.Curt.:Fr.) Lloyd (Aphyllophoromycetideae) Cooperatively Induces Tumor Necrosis Factor-α and Interleukin-6 with Lipopolysaccharide by Binding to Dectin-1
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 1&2
Mi Jeong Kim, Ha Won Kim, Se Young Choung, Dong Hee Lee, Byong Kak Kim, Su Kyoung Sung
The Anti-Inflammatory Effects of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Higher Basidiomycetes) in a Coculture System of 3T3-L1 Adipocytes and RAW264 Macrophages
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 7
Noriyasu Hirasawa, Kenji Ouchi, Koichiro Mori
Macrophages Stimulated by Polysaccharide Purified from Agaricus brasiliensis S.Wasser et al. (Agaricomycetideae) Enhance mRNA Expression of Th1 Cytokine Including IL-12 and 18
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 4
Nobuhide Fujitake, Sachiko Kawakami, Masashi Mizuno
Dectin-1 and GM-CSF on Immunomodulating Activities of Fungal 6-Branched 1,3-β-Glucans
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 2
Naohito Ohno, Toshie Harada