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International Journal of Medicinal Mushrooms
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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i4.70
pages 409-418

Submerged and Solid-State Cultivation of Bioactive Extra- and Intracellular Polysaccharides of Medicinal Mushrooms Ganoderma lucidum (W. Curt.: Fr.) P. Karst. and Grifola frondosa (Dicks.: Fr.) S. F. Gray (Aphyllophoromycetideae)

Jozica Habijanic
National Institute of Chemistry, Ljubljana, Slovenia ; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia
Mirjan Svagelj
Department of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana
Marin Berovic
Faculty of Chemistry and Chemical Engineering Department of Chemical and Biochemical Engineering University of Ljubljana Askerceva 5, 1001 Ljubljana, Slovenia
Bojana Boh
Faculty of Natural Sciences and Engineering, University of Ljubljana, Ljubljana, Slovenia
Branka Wraber
Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia

ABSTRAKT

Original strains of Ganoderma lucidum (MZKI G97) and Grifola frondosa (GF3) isolated from Slovenian forests were cultivated using submerged and solid-state cultivation. In G. lucidum after 14 days of submerged fed-batch cultivation, up to 17.0 g L−1 dry fungal biomass was produced. Extracellular (1.7 g L−1) and intracellular (0.45 g L−1) polysaccharide fractions were isolated, while in 18 days of solid-state cultivation, 5.77 mg/g of extracellular and 1.45 mg/g of intracellular polysaccharide were produced at the end of the cultivation period. In 28 days of submerged cultivation of G. frondosa, 3.65 mg L−1 of extracellular and 1.30 mg L−1 of intracellular polysaccharide were produced. In 38 days of solid-state cultivation, 3.80 mg/g of extracellular and 0.70 mg/g of intracellular polysaccharide were produced. Polysaccharides were further separated by ion-exchange, gel, and affinity chromatography. The isolated polysaccharides were mainly β-D-glucans. Immunostimulatory effects of isolates were tested for induction of cytokine (TNF-α and IFN-γ) synthesis in human peripheral blood mononuclear cells (PBMC) isolated from buffy coat.


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