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International Journal of Medicinal Mushrooms
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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v18.i2.80
pages 177-189

Screening of Indian Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes): A UPC2-SQD-MS Approach

Anuja Bhardwaj
Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, Timarpur, Delhi, India
Mousami Srivastava
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India
Mamta Pal
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India
Yogesh Kumar Sharma
Waters (India) Pvt. Ltd., Bangalore, India
Saikat Bhattacharya
Waters (India) Pvt. Ltd., Bangalore, India
Rajkumar Tulsawani
Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi, India
Ragumani Sugadev
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India

ABSTRAKT

Oriental medicinal mushroom Ganoderma lucidum has been widely used for the promotion of health and longevity owing to its various bioactive constituents. Therefore, comprehending metabolomics of different G. lucidum parts could be of paramount importance for investigating their pharmacological properties. Ultra-performance convergence chromatography (UPC2) along with mass spectrometry (MS) is an emerging technique that has not yet been applied for metabolite profiling of G. lucidum. This study has been undertaken to establish metabolomics of the aqueous extracts of mycelium (GLM), fruiting body (GLF), and their mixture (GLMF) using ultra-performance convergence chromatography single quadrupole mass spectrometry (UPC2-SQD-MS). Aqueous extracts of G. lucidum prepared using an accelerated solvent extraction technique have been characterized for their mycochemical activities in terms of total flavonoid content, 1,1-diphenyl-2-picryl-hydrazyl scavenging activity, and ferric ion reducing antioxidant power. The UPC2-SQD-MS technique has been used for the first time for metabolite profiling of G. lucidum on a Princeton Diol column (4.6 × 250 mm; 5 µm) using supercritical CO2 (solvent) and 20 mM ammonium acetate in methanol (co-solvent). In the present study, UPC2-SQD-MS was found to be a rapid, efficient, and high-throughput analytical technique, whose coupling to principal component analysis (PCA) and phytochemical evaluation could be used as a powerful tool for elucidating metabolite diversity between mycelium and fruiting body of G. lucidum. PCA showed a clear distinction in the metabolite compositions of the samples. Mycochemical studies revealed that overall GLF possessed better antioxidant properties among the aqueous extracts of G. lucidum.


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