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International Journal of Medicinal Mushrooms
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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2017021274
pages 675-684

Isolation and Characterization of Lectin from the Artist's Conk Medicinal Mushroom, Ganoderma applanatum (Agaricomycetes), and Evaluation of Its Antiproliferative Activity in HT-29 Colon Cancer Cells

Sekar Kumaran
Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia; Muthayammal Center for Advanced Research, Muthayammal College of Arts & Science, Rasipuram, Tamil Nadu, India; S&&R Agro Bio-Tech, Chennai, Tamil Nadu, India; Department of Botany, Periyar University Constituent Arts and Science College, Harur, Tamil Nadu, India
Ashok Kumar Pandurangan
Muthayammal Center for Advanced Research, Muthayammal College of Arts & Science, Rasipuram, Tamil Nadu, India; Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti of Putra Malaya, Serdang, Selangor, Malaysia
Ramalingam Shenbhagaraman
S&R Agro Bio-Tech, Chennai, Tamil Nadu, India
Norhaizan Mohd. Esa
Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti of Putra Malaya, Serdang, Selangor, Malaysia

ABSTRAKT

The growth and lectin production of Ganoderma applanatum, a white rot fungus, was optimized in broth cultures. The fungus was found to have a higher growth rate and higher lectin activity when grown in a medium adjusted to pH 6.5 at 26°C under stationary conditions. Expression of lectin activity started in 5-day-old mycelial culture; maximum activity was expressed after the 15th day of incubation. Among the various carbon and nitrogen sources tested, the carbon source sucrose and the nitrogen source yeast extract support maximum growth and lectin production. Lectin from G. applanatum was purified by ammonium sulfate precipitation and ion exchange chromatography. The purified fraction revealed a single band with a molecular weight of 35.0 kDa. Moreover, carbohydrates such as mannitol, glucose, sucrose, maltose, mannose, galactose, sorbose, and fructose were found to inhibit the hemagglutinating activity of the lectin. The purified lectins from G. applanatum contain cytotoxic and proapoptotic activities against HT-29 colon adenocarcinoma cells.


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