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International Journal of Medicinal Mushrooms
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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018028430
pages 1163-1172

Expression and Antitumor Function of Latcripin-4 RCC1 and ANK Domain Protein on HepG2 from the Shiitake Medicinal Mushroom, Lentinus edodes C91–3 (Agaricomycetes), Transcriptome

Xuefang Guo
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Xiaoli Wang
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Min Liu
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Lei Chen
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Warren Chanda
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Arshad Ahmed Pahiar
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Xingyun Li
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Wei Zhang
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Anhong Ning
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Min Huang
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Jing Cao
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Mintao Zhong
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China

ABSTRAKT

The recombinant protein of Latcripin-4 regulator of chromosome condensation 1 (RCC1) and ankyrin (ANK) domains were expressed and the antitumor activity of Latcripin-4 on HepG2 cells was studied. First, the Latcripin-4 transcript was selected from the medicinal mushroom Lentinus edodes C91–3 transcriptome by bioinformatics. Then the full-length gene of Latcripin-4 was isolated with 3'-full rapid amplification of cDNA ends (RACE) and 5'-full RACE methods according to the transcriptome. The RCC1 and ANK domains from the full-length gene were selected and inserted into the expression vector pET-32a (+) and expressed in Escherichia coli Rosetta-gami (DE3). Western blotting indicated that the protein was expressed successfully. The biological function of Latcripin-4 RCC1 and ANK domain protein on HepG2 cells was studied with the CCK-8 assay. All results demonstrated that Latcripin-4 RCC1 and ANK domain protein can inhibit the growth of human HepG2 liver cancer cells, which brings new insights to identifying antitumor proteins from medicinal food for cancer therapy.


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