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International Journal of Medicinal Mushrooms
Impact-faktor: 1.423 5-jähriger Impact-Faktor: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

Volumes:
Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019030388
pages 367-380

Mitigating Acetaminophen-Induced Hepatotoxicity by Using a Water-Alcohol Extract of Phellinus caryophylli (Agaricomycetes) in a Murine Model

Yogesh Bharat Dalvi
Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences and Research Centre, Tiruvalla, Kerala, India
Ruby Varghese
Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences and Research Centre, Tiruvalla, Kerala, India; MACFAST, Tiruvalla, Kerala, India
Prasad Y. Lamrood
Department of Botany, Ahmednagar College (affiliated with Savitribai Phule Pune University), Ahmednagar, Maharashtra, India
Cherupally Krishnan Krishnan Nair
Mar Athanasious College for Advanced Studies, Tiruvalla, Tiruvalla, Kerala, India; St.Gregorios Dental College & Research Centre, Kothamangalam, Kerala, India

ABSTRAKT

This study investigates the hepatoprotective effect of a water-alcohol extract of the medicinal mushroom Phellinus caryophylli (Racib.) G. Cunn. (PCE) against acetaminophen (APAP)-induced hepatotoxicity in Swiss albino mice. The mice orally received APAP (150 mg/kg body weight), followed by PCE extract (250 or 500 mg/kg body weight). The liver damage induced by APAP was analyzed on the basis of blood serum parameters (glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, and alkaline phosphatase), antioxidant assays (reduced glutathione and glutathione peroxidase), and tissue peroxidation based on malondialdehyde level. The molecular mechanism underlying the prevention of APAP-induced damage by PCE was also analyzed. Liver damage was confirmed on the basis of increased serum parameter values, decreased antioxidant levels, and cellular and molecular alterations, which PCE restored in a dose-dependent manner. At a transcriptional level, PCE downregulated expression of the preapoptototic gene Bax and the inflammatory gene Cox2 but upregulated the antiapoptotic gene Bcl2 in the mice that received APAP. PCE exerted a hepatoprotective effect by preventing apoptotic and inflammatory events caused by APAP. Thus, this study demonstrates a hepatoprotective effect of PCE, which could be explored further for managing hepatopathy.

REFERENZEN

  1. Wasser SP. , Medicinal mushroom science: history, current status, future trends, and unsolved problem. Int J Med Mushrooms. 2010;12(1):1–16.

  2. Wasser SP. , Medicinal mushroom science: current perspectives, advances, evidences, and challenges. Biomed J. 2014;37(6):345–56.

  3. Patel S, Goyal A. , Recent developments in mushrooms as anti-cancer therapeutics: a review. 3 Biotech. 2012;2(1):1–15.

  4. Martel J, Ojcius DM, Chang CJ, Lin CS, Lu CC, Ko YF, Tseng SF, Lai HC, Young JD. , Anti-obesogenic and antidiabetic effects of plants and mushrooms. Nat Rev Endocrinol. 2017;13(3):149–60.

  5. Alves MJ, Ferreira IC, Dias J, Teixeira V, Martins A, Pintado M. , A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Planta Med. 2012;78:1707–18.

  6. Lindequist U, Niedermeyer TH, Jülich WD. , The pharmacological potential of mushrooms. Evid Based Complement Alternat Med. 2005;2(3):285–99.

  7. Reshetnikov SV, Tan KK, Wasser SP. , Higher Basidiomycota as a source of antitumor and immunostimulating polysaccharides. Int J Med Mushrooms. 2001;3(4):361–94.

  8. Wasser SP. , Medicinal mushrooms in human clinical studies. Part I. Anticancer, oncoimmunological, and immunomodulatory activities: a review. Int J Med Mushrooms. 2017;19(4):279–317.

  9. Wangkheirakpam S, Deb L, Joshi DD, Leishangthem GD, Biswas D. , Hepatoprotective effect of Auricularia delicata (Agaricomycetes) from India in rats: biochemical and histopathological studies and antimicrobial activity. Int J Med Mushrooms. 2018;20(3):213–25.

  10. Chen TQ, Wu JG, Kan YJ, Yang C, Wu YB, Wu JZ. , Antioxidant and hepatoprotective activities of crude polysaccharide extracts from lingzhi or reshi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), by ultrasonic-circulating extraction. Int J Med Mushrooms. 2018;20(6):581–93.

  11. Nisar J, Mustafa I, Anwar H, Sohail MU, Hussain G, Ullah MI, Faisal MN, Bukhari SA, Basit A., Shiitake culinary-medicinal mushroom, Lentinus edodes (Agaricomycetes): a species with antioxidant, immunomodulatory, and hepatoprotective activities in hypercholesterolemic rats. Int J Med Mushrooms. 2017;19(11):981–90.

  12. Ooi VE. , Hepatoprotective effect of some edible mushrooms. Phytother Res. 1996;10(6):536–8.

  13. Vaidya JG, Rabba AS. , Fungi in folk medicine. Mycologist. 1993;7(3):131–3.

  14. Vaidya JG, Lamrood PY. , Traditional medicinal mushrooms and fungi of India. Int J Med Mushrooms. 2000;2(3):209–14.

  15. Ajith TA, Janardhanan KK. , Antioxidant and anti-inflammatory activities of methanol extract of Phellinus rimosus (Berk.) Pilat. Indian J Exp Biol. 2001;39(11):1166–9.

  16. Ye L, Zheng H, Zhang Z, Yang Y., Preparative Isolation of 5 antioxidant constituents from the medicinal mushroom Phellinus baumii (Agaricomycetes) by high-speed countercurrent chromatography and preparative high-performance liquid chromatography. Int J Med Mushrooms. 2017;19(4):319–26.

  17. Kovacs B, Zomborszki ZP, Orban-Gyapai O, Csupor-Loffler B, Liktor-Busa E, Lazar A, Papp V, Urban E, Hohmann J, Vanyolos A., Investigation of antimicrobial, antioxidant, and xanthine oxidase–inhibitory activities of Phellinus (Agaricomycetes) mushroom species native to Central Europe. Int J Med Mushrooms. 2017;19(5):387–94.

  18. Sung SK, Batbayar S, Lee DH, Kim H. , Activation of NADPH oxidase by beta-glucan of Phellinus baumii Pilat (Hymenochaetaceae, higher Basidiomycetes) in RAW264.7 cells. Int J Med Mushrooms. 2017;19(11):957–65.

  19. Jiang Z, Jin M, Zhou W, Li R, Zhao Y, Jin X, Li G. , Anti-inflammatory activity of chemical constituents isolated from the willow bracket medicinal mushroom Phellinus igniarius (Agaricomycetes). Int J Med Mushrooms. 2018;20(2):119–28.

  20. Yamac M, Zeytinoglu M, Senturk H, Kartkaya K, Kanbak G, Bayramoglu G, Oglakci A, Van Griensven LJ., Effects of black hoof medicinal mushroom, Phellinus linteus (Agaricomycetes), polysaccharide extract in streptozotocin-induced diabetic rats. Int J Med Mushrooms. 2016;18(4):301–11.

  21. Rony KA, Ajith TA, Janardhanan KK. , Hypoglycemic and hypolipidemic effects of the cracked-cap medicinal mushroom Phellinus rimosus (higher Basidiomycetes) in streptozotocin-induced diabetic rats. Int J Med Mushrooms. 2015;17(6):521–31.

  22. Lee JW, Baek SJ, Bae WC, Park JM, Kim YS. , Antitumor and antioxidant activities of the extracts from fruiting body of Phellinus linteus. Mycobiology. 2006;34(4):230–5.

  23. Yoon SK, Sung SK, Lee DH, Kim HW. , Tissue inhibitor of metalloproteinase-1 (TIMP-1) and IL-23 induced by polysaccharide of the black hoof medicinal mushroom, Phellinus linteus (Agaricomycetes). Int J Med Mushrooms. 2017;19(3):213–23.

  24. Wang J, Chen B, Hu F, Zou X, Yu H, Wang J, He H, Zhang H, Huang W., Effect of hispolon from Phellinus lonicerinus (Agaricomycetes) on estrogen receptors, aromatase, and cyclooxygenase II in MCF-7 breast cancer cells. Int J Med Mushrooms. 2017;19(3):233–42.

  25. Xue Q, Sun J, Zhao M, Zhang K, Lai R. , Immunostimulatory and anti-tumor activity of a water-soluble polysaccharide from Phellinus baumii mycelia. World J Microbiol Biotechnol. 2011;27(5):1017–23.

  26. Kim JH, Choi BY, Kim HJ, Kim IY, Lee BE, Sohn M, Park HJ, Suh SW. , A water-ethanol extract from the willow bracket mushroom, Phellinus igniarius (higher Basidiomycetes), reduces transient cerebral ischemia-induced neuronal death. Int J Med Mushrooms. 2015;17(9):879–89.

  27. Hall JE. , Guyton and Hall textbook of medical physiology. 13th ed. Philadelphia (PA): Saunders; 2016.

  28. Mattia A, Coluzzi F. , What anesthesiologists should know about paracetamol (acetaminophen). Minerva Anestesiol. 2009;75(11):644–53.

  29. Rumack BH, Peterson RC, Koch GG, Amara IA. , Acetaminophen overdose: 662 cases with evaluation of oral acetylcysteine treatment. Arch Intern Med. 1981;141(3):380–5.

  30. Rony KA, Ajith TA, Mathew J, Janardhanan KK., The medicinal cracked-cap polypore mushroom Phellinus rimosus (higher Basidiomycetes) attenuates alloxan-induced hyperglycemia and oxidative stress in rats. Int J Med Mushrooms. 2013;15(3):287–300.

  31. Larsen MJ, Cobb-Poulle LA. , Phellinus (Hymenochaetaceae): a survey of the world taxa. Synopsis Fungorum 3. Oslo (Norway): Fungiflora; 1990.

  32. Sadasivam S, Manickam A. , Biochemical methods. 2nd ed. New Delhi (India): New Age International; 1996.

  33. Garrat DC. , The quantitative analysis of drugs. 3rd ed. London (UK): Chapman and Hall; 1964.

  34. Benzie IF, Strain JJ. , The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem. 1996;239(1):70–6.

  35. Verma PK, Raina R, Sultana M, Singh M, Kumar P. , Acetaminophen induced oxidative and histopathological alterations in hepatic tissue: protective effects of Alstonia scholaris leaf extracts. Pharmacogn J. 2016;8(4):385–91.

  36. Nagalekshmi R, Menon A, Chandrasekharan DK, Nair CK. , Hepatoprotective activity of Andrographis paniculata and Swertia chirayita. Food Chem Toxicol. 2011;49(12):3367–73.

  37. Reitman S, Frankel S. , A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957;28(1):56–63.

  38. Kind PR, King EJ. , Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol. 1954;7(4):322–6.

  39. Buege JA, Aust SD. , Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302–10.

  40. Hafeman DG, Sunde RA, Hoekstra WG. , Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutr. 1974;104(5):580–7.

  41. Moron MS, Depierre JW, Mannervik B. , Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta. 1979;582(1):67–78.

  42. Li R, Ding C, Zhang J, Xie M, Park D, Ding Y, Chen G, Zhang G, Gilbert-Ross M, Zhou W, Marcus AI. , Modulation of BAX and mTOR for cancer therapeutics. Cancer Res. 2017;77(11):3001–12.

  43. Yang Y, Xie Y, Wu M, Geng Y, Li R, Xu L, Liu X, Pan Y. , Expression of mmu-miR-96 in the endometrium during early pregnancy and its regulatory effects on stromal cell apoptosis via BCL2. Mol Med Rep. 2017;15(4):1547–54.

  44. Xu Y, Cardell LO. , Nicotine impairs cyclooxygenase-2-dependent kinin-receptor-mediated murine airway relaxations. Toxicol Appl Pharmacol. 2014;275(1):12–21.

  45. Ritz JM, Kühle O, Riethdorf S, Sipos B, Deppert W, Englert C, Günes C. , A novel transgenic mouse model reveals humanlike regulation of an 8-kbp human TERT gene promoter fragment in normal and tumor tissues. Cancer Res. 2005;65(4):1187–96.

  46. Chomczynski P, Sacchi N. , The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nat Protoc. 2006;1(2):581–5.

  47. Livak KJ, Schmittgen TD. , Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 2001;25(4):402–8.

  48. Brzoska MM, Moniuszko-Jakoniuk J, Piłat-Marcinkiewicz B, Sawicki B. , Liver and kidney function and histology in rats exposed to cadmium and ethanol. Alcohol Alcohol. 2003;38(1):2–10.

  49. Ganesh PN, Leelavathy KM. , New records of Phellinus from India. Curr Sci. 1986;55(15):727–8.

  50. Lung MY, Tsai JC, Huang PC. , Antioxidant properties of edible basidiomycete Phellinus igniarius in submerged cultures. J Food Sci. 2010;75(1):E18–24.

  51. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. , Oxidative stress and antioxidant defense. World Allergy Organ J. 2012;5(1):9–19.

  52. Ajith TA, Sheena N, Janardhanan KK. , Phellinus rimosus. Protects carbon tetrachloride-induced chronic hepatotoxicity in rats: antioxidant defense mechanism. Pharm Biol. 2006;44(6):467–74.

  53. Park SC, Cheon YP, Son WY, Rhee MH, Kim TW, Song JC, Kim KS. , Hepatoprotective effects of polysaccharides isolated from Phellinus gilvus against carbon tetrachloride-induced liver injury in rats. Toxicol Res. 2009;25(1):29–33.

  54. Ray SD, Mumaw VR, Raje RR, Fariss MW. , Protection of acetaminophen-induced hepatocellular apoptosis and necrosis by cholesteryl hemisuccinate pretreatment. J Pharmacol Exp Ther. 1996;279(3):1470–83.

  55. Bartels AL, Leenders KL. , Cyclooxygenase and neuroinflammation in Parkinson’s disease neurodegeneration. Curr Neuropharmacol. 2010;8(1):62–8.


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