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International Journal of Medicinal Mushrooms
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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v16.i3.40
pages 239-245

In Vitro Anti-proliferative Effects on NB4 Human Leukemia Cells and Physicochemical Screening of Pleurotus sp. (Higher Basidiomycetes) Mycelia from Cuba

Humberto J. Morris
Centre of Studies for Industrial Biotechnology, Universidad de Oriente, Santiago de Cuba, Cuba
Edgar Hernandez
Department of Biology, Faculty of Natural Sciences, Universidad de Oriente, Santiago de Cuba 5. CP 90 500, Cuba
Gabriel Llaurado
Centre of Studies for Industrial Biotechnology, Universidad de Oriente, Santiago de Cuba, Cuba
Maria Cristina Tejedor
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Pilar Sancho
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Angel Herraez
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Maria del Carmen Boyano-Adanez
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Ana Isabel Garcia-Perez
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain
Jose Carlos Diez
Department of Biochemistry and Molecular Biology, University of Alcala de Henares, Madrid, Spain

ABSTRAKT

This study examined the phytochemical profile and the in vitro anti-proliferative effects of a hot water mycelial extract from the edible mushroom Pleurotus sp. on NB4 human leukemia cells. Flow-cytometry analyses were used to measure cell viability, cell cycle, and apoptosis in cells incubated 24 h with the extract at doses of 100 and 200 µg/mL. Pleurotus sp. extract reduced cell viability, particularly at the concentration of 200 µg/mL to 82% compared to control cells, and induced apoptosis demonstrated by an increase in the number of annexin V-FITC+ cells (25% at 200 µg/mL). The NB4 cells were arrested in the G2/M phase thus supporting a cell-cycle dependent anticancer mechanism. Although carbohydrates (76.8%, w/w) appear to be the most important antitumor compound, secondary metabolites-like phenolics would also contribute to the anti-proliferative activity. The results indicate that Pleurotus sp. mycelia obtained by submerged fermentation may be an interesting renewable resource for developing functional foods and new antitumor therapeutic agents.


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