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Critical Reviews™ in Eukaryotic Gene Expression
Impact-faktor: 2.156 5-jähriger Impact-Faktor: 2.255 SJR: 0.649 SNIP: 0.599 CiteScore™: 3

ISSN Druckformat: 1045-4403
ISSN Online: 2162-6502

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukarGeneExpr.v6.i2-3.90
pages 285-297

Regulation of the Chicken Lysozyme Locus in Transgenic Mice

Constanze Bonifer
Institut fur Biologie III, Universitat Freiburg, Schanzlestr.1, D-79104 Freiburg i. Br., Germany
Matthias C. Huber
Institut fur Biologie III, Universitat Freiburg, Schanzlestr.1, D-79104 Freiburg i. Br., Germany
Nicole Faust
Institut fur Biologie III, Universitat Freiburg, Schanzlestr.1, D-79104 Freiburg i. Br., Germany
Albrecht E. Sippel
Institut fur Biologie III, Universitat Freiburg, Schanzlestr.1, D-79104 Freiburg i. Br., Germany

ABSTRAKT

The chicken lysozyme locus is transcriptionally activated during macrophage differentiation. Each cis-regulatory element has its unique activation stage during cell differentiation, whereby maximal transcriptional activity of the gene is only observed when all cis-elements are active. The complete chicken lysozyme locus is expressed position independently and at a high level in macrophages of transgenic mice. For correct transgene regulation, the cooperation of all cis-regulatory elements is required. These cis-regulatory elements specify the mode of regulation and we observe the same expression pattern of the transgene in the mouse and the endogenous gene in chicken macrophages. This indicates that the transcription factors responsible for chicken lysozyme regulation are highly conserved in evolution. The endogenous mouse lysozyme gene is regulated differently. The chromatin of the lysozyme locus is highly structured in the transcriptionally active, as well as in the inactive state. The transcriptional activation of the lysozyme locus is accompanied by extensive chromatin rearrangements, which are disturbed when one essential cis-regulatory element is deleted and the transgenes are subjects to genomic position effects. Based on these results, we propose that a distinct chromatin architecture of a gene locus is required for its correct activation.


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