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Plasma Medicine

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ISSN Druckformat: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Effect of Oxygen on Decontamination of Cumin Seeds by Atmospheric Pressure Dielectric Barrier Discharge Plasma

Volumen 6, Ausgabe 3-4, 2016, pp. 339-347
DOI: 10.1615/PlasmaMed.2017019140
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ABSTRAKT

We studied the effect of oxygen on decontamination of cumin seeds by atmospheric pressure dielectric barrier discharge (DBD) plasma. A peak-to-peak 15-kV radio-frequency power supply at 17-kHz frequency was used to generate Ar and Ar-O2 plasmas between two circular electrodes. Electrodes were covered by mica plates with a 5-mm gap between each plate. Seeds were exposed to plasma until they were completely decontaminated. To determine total bacteria content, the seeds were cultured using a pour-plate method on a nutrient agar medium. To evaluate bacterial destruction, concentrations of double-stranded DNA and protein were determined spectrophotometrically. Results indicate that the surviving microorganism density decreased significantly after exposure of Ar and Ar-02 plasma in a time-dependent manner. Complete elimination of total bacteria was obtained after 20 and 40 min of exposure for Ar-O2 and Ar plasma, respectively. Our research shows that atmospheric pressure DBD plasma using Ar or Ar-O2 gas may be a suitable alternative method for decontamination of cumin seeds, without resulting in any detrimental effects on food quality of seeds.

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