Erscheint 12 Ausgaben pro Jahr
ISSN Druckformat: 1091-028X
ISSN Online: 1934-0508
Indexed in
Protein Crystal-Mediated Biotemplating
ABSTRAKT
Protein crystals, routinely prepared for the elucidation of protein 3-D structure by X-ray crystallography, present a highly accurate 3-D array of protein molecules. Along with the formation of this array, a complementary 3-D array of voids is also formed, with pattern, geometry, and size depending on the protein building-block and its packaging within the crystal. Here we present recent results demonstrating the feasibility of using the pores of protein crystals as a biotemplate for the preparation of composite materials. Our previously developed methods of monitoring the preservation of the 3-D protein crystal template throughout biotemplating of chemically cross-linked hydrogels and the elucidation of the mechanism of protein crystal cross-linking by glutaraldehyde were applied in this study to enable and demonstrate the feasibility of protein crystal-mediated biotemplating resulting in a 3-D array of silver particles located within the pores of the crystal. Our accumulated results indicate that the pool of protein crystals may be successfully exploited as a source of biotemplates for the fabrication of novel composite materials, in contrast to the common consideration of protein crystals as labile structures, useful only for the elucidation of the 3-D structure of proteins.
-
Wine Yariv, Cohen-Hadar Noa, Lamed Raphael, Freeman Amihay, Frolow Felix, Modification of protein crystal packing by systematic mutations of surface residues: Implications on biotemplating and crystal porosity, Biotechnology and Bioengineering, 104, 3, 2009. Crossref
-
Cohen-Hadar Noa, Lagziel-Simis Shira, Wine Yariv, Frolow Felix, Freeman Amihay, Re-structuring protein crystals porosity for biotemplating by chemical modification of lysine residues, Biotechnology and Bioengineering, 108, 1, 2011. Crossref
-
Buch Michal, Wine Yariv, Dror Yael, Rosenheck Sonia, Lebendiker Mario, Giordano Rita, Leal Ricardo M.F., Popov Alexander N., Freeman Amihay, Frolow Felix, Protein products obtained by site-preferred partial crosslinking in protein crystals and “liberated” by redissolution, Biotechnology and Bioengineering, 111, 7, 2014. Crossref
-
Yan Er-Kai, Cao Hui-Ling, Zhang Chen-Yan, Lu Qin-Qin, Ye Ya-Jing, He Jin, Huang Lin-Jun, Yin Da-Chuan, Cross-linked protein crystals by glutaraldehyde and their applications, RSC Advances, 5, 33, 2015. Crossref
-
Freeman Amihay, Protein-Mediated Biotemplating on the Nanoscale, Biomimetics, 2, 4, 2017. Crossref
-
Yamada Yohei, Toyama Shota, Yabutani Tomoki, Effects of solvents on the crystal structure of cross-linked lysozyme crystals, International Journal of Modern Physics B, 32, 19, 2018. Crossref
-
Boncli Y., biotemplating, in Catalysis from A to Z, 2020. Crossref
-
Herrera-Beurnio M. Carmen, Hidalgo-Carrillo Jesús, López-Tenllado Francisco J., Martin-Gómez Juan, Estévez Rafael C., Urbano Francisco J., Marinas Alberto, Bio-Templating: An Emerging Synthetic Technique for Catalysts. A Review, Catalysts, 11, 11, 2021. Crossref
-
Hartje Luke F., Munsky Brian, Ni Thomas W., Ackerson Christopher J., Snow Christopher D., Adsorption-Coupled Diffusion of Gold Nanoclusters within a Large-Pore Protein Crystal Scaffold, The Journal of Physical Chemistry B, 121, 32, 2017. Crossref