Abo Bibliothek: Guest
Journal of Environmental Pathology, Toxicology and Oncology

Erscheint 4 Ausgaben pro Jahr

ISSN Druckformat: 0731-8898

ISSN Online: 2162-6537

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 2.4 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 2.8 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.5 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00049 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.59 SJR: 0.429 SNIP: 0.507 CiteScore™:: 3.9 H-Index: 49

Indexed in

Discrimination by RT-Nested PCR of Alpha-Lactalbumin Transcript Expression in Mammary Tumors

Volumen 38, Ausgabe 4, 2019, pp. 313-327
DOI: 10.1615/JEnvironPatholToxicolOncol.2019030172
Get accessGet access

ABSTRAKT

Alpha-lactalbumin is a protein of milk expressed by mammary epithelial cells and by some breast tumors. The alpha-lactalbumin gene has two transcripts. The expression of transcript 2 has not yet been studied. The main objective of this paper was to establish the expression profile of alpha-lactalbumin at the mRNA level and to develop a technique discriminating between the two transcripts. The study was performed on 46 fresh mammary biopsies: 17 malignant tumors, 13 benign tumors, and 16 adjacent healthy tissues. We developed an RT-nested PCR to detect LALBA gene expression without distinction between transcripts. We also designed an RT-nested PCR that detects transcript 2. Both nested PCRs avoid amplification of potentially contaminating genomic DNA. We show that benign tumors tend to appear at a young age contrarily to malignant tumors appearing later with p value = 0.002. Moreover, LALBA transcript expression varies among tissues more important in benign (69%) than in malignant (35%) tumors with significant p value = 0.041. The intensity of the bands reflected differential expression between patients with concordant data between both RT-nested PCRs. We also performed a qualitative analysis according to histopathological parameters and molecular subtypes. The expression of transcript 2 appeared associated with the HER2+ subtype, while transcript 1 was associated with the luminal A and triple-negative subtypes. In conclusion, we designed specific and sensitive methods to detect LALBA transcripts. We show for the first time a differential expression of these transcripts, but we need to confirm their potential use as markers in breast cancer.

REFERENZEN
  1. Brodbeck U, Denton WL, Tanahashi N, Ebner KE. The isolation and identification of the B protein of lactose synthe-tase as a-lactalbumin. J Bio Chem. 1967;242(7):1391-7.

  2. Kleinberg DL, Todd J. a-Lactalbumin in human and subhuman primate normal mammary tissue and in human breast cancer as a marker for prolactin activity. Cancer Res. 1978;38:4318-22.

  3. Clayton F, Ordonez NG, Hanssen GM, Hanssen H. Immunoperoxidase localization of lactalbumin in malignant breast neoplasms. Arch Pathol Lab Med. 1982; 106(6): 268-70.

  4. Kleinberg DL, Todd J, Groves ML. Studies on human a-lactalbumin: radioimmunoassay measurements in normal human breast and breast cancer. J Clin Endocrinol Metab. 1977;45:1238-50.

  5. Martin RH, Glass MR, Chapman C, Wilson GD, Woods KL. Human a-lactalbumin and hormonal factors in pregnancy and lactation. Clin Endocrinol. 1980;13:23-30.

  6. Balley A, Sloane JP, Trickey BS, Ormerod MG. An immunocytochemical study of a-lactalbumin in human breast tissue. J Pathol. 1982;137:13-23.

  7. Thean ET, Toh BH. Serum human a-lactalbumin as a marker for breast cancer. Br J Cancer. 1990;61:773-5.

  8. Kleinberg DL. Human alpha-lactalbumin: measurement in serum and in breast cancer organ cultures by radioimmunoassay. Science. 1975;190(4211):276-8.

  9. Schultz GS, Ebner KE. a-lactalbumin levels in human mammary tumors, sera, and mammary cell culture lines. Cancer Res. 1977;37:4489-92.

  10. Lee AK, DeLellis RA, Rosen PP, Herbert-Stanton T, Tallberg K, Garcia C, Wolfe HJ. Alpha-lactalbumin as an immunohistochemical marker for metastatic breast carcinomas. Am J Surg Pathol. 1984;8(2):93-100.

  11. Bahu RM, Mangkornkanok-Mark M, Albertson D, Fors E, Molteni A, Battifora H. Detection of alpha-lactalbumin in breast lesions and relationship to estrogen receptors and serum prolactin. Cancer. 1980;46(8):1775-80.

  12. Le Doussal V, Zangerle PF, Collette J, Spyratos F, Hacene K, Briere M, Gest J, Franchimont P. Immunohistochemical detection of alpha-lactalbumin in breast lesions. Eur J Cancer Clin Oncol. 1984;20(8):1069-78.

  13. Perou CM, Sorlie T, Eisen MB, Van de Rijn M, Jeffrey SS, Rees CA, Pollack JR, Ross DT, Johnsen H, Akslen LA, Fluge 0, Pergamenschikov A, Williams C, Zhu SX, L0nning PE, B0rresen-Dale AL, Brown PO, Botstein D. Molecular portraits of human breast tumours. Nature. 2000;406:747-52.

  14. Tuohy VK, Jaini R, Johnson JM, Loya MG, Wilk D, Downs-Kelly E, Mazumder S. Targeted vaccination against human a-lactalbumin for immunotherapy and primary immunoprevention of triple negative breast cancer. Cancers. 2016;8:pii:E56.

  15. Hall L, Craig R, Campbell P. mRNA species directing syn-thesis of milk proteins in normal and tumour tissue from human mammary gland. Nature. 1979;277(5691):54-6.

  16. Davies MS, West LF, Davis MB, Povey S, Craig RK. The gene for human alpha-lactalbumin is assigned to chromosome 12q13. Annals Human Gen. 1987;51:183-8.

  17. Zerbino DR, Achuthan P, Akanni W, Amode MR, Barrell D, Bhai J, Billis K, Cummins C, Gall A, Garda Giron C, Gil L, Gordon L, Haggerty L, Haskell E, Hourlier T, Izuogu OG, Janacek SH, Juettemann T, Kiang To J, Laird MR, Lavidas I, Liu Z, Loveland JE, Maurel T, McLaren W, Moore B, Mudge J, Murphy DN, Newman V, Nuhn M, Ogeh D, Kee Ong C, Parker A, Patricio M, Singh Riat H, Schuilenburg H, Sheppard D, Sparrow H, Taylor K, Thormann A, Vullo A, Walts B, Zadissa A, Frankish A, Hunt SE, Kostadima M, Langridge N, Martin FJ, Muffato M, Perry E, Ruffier M, Staines DM, Trevanion SJ, Aken BL, Cunningham F, Yates A, Flicek P. Ensembl 2018. Nucleic Acids Res. 2018;46:D754-D761.

  18. Horibe D, Ochiai T, Shimada H, Tomonaga T, Nomura F, Tanizawa T, Hayashi H. Rapid detection of metastasis of gastric cancer using reverse transcription loop-mediated isothermal amplification. Int J Cancer. 2006;120: 1063-9.

  19. Woods KL, Cove DH, Howell A. Predictive classification of human breast carcinomas based on lactalbumin synthesis. Lancet. 1977;310:14-16.

  20. Simfckova M, Lang BA, Rejthar A, Cernoch M, Saka- lova J, Pacovsky Z. Immunohistochemical localization of alpha-lactalbumin in human breast cancer tissue. Neoplasma. 1989;36(1):11-20.

REFERENZIERT VON
  1. Rincón-Riveros Andrés, De la Peña Jairo, Rubiano Wilson, Olivella Fabio, Martinez-Agüero María, Villegas Victoria E., Primary Breast Angiosarcoma: Comparative Transcriptome Analysis, International Journal of Molecular Sciences, 23, 24, 2022. Crossref

  2. Ahmad Ejaj, Ali Asgar, Nimisha , Kumar Sharma Abhay, Ahmed Firoz, Mehdi Dar Ghulam, Mohan Singh Anand, Apurva , Kumar Arun, Athar Alina, Parveen Farha, Mahajan Bhawna, Singh Saluja Sundeep, Molecular approaches in cancer, Clinica Chimica Acta, 537, 2022. Crossref

823 Artikelansichten 298 Artikel-Downloads Metriken
823 ANSICHTEN 298 HERUNTERLADEN 2 Crossref ZITATE Google
Scholar
ZITATE

Artikel mit ähnlichem Inhalt:

Gene Expression Profiling of Di-n-Butyl Phthalate in Normal Human Mammary Epithelial Cells Journal of Environmental Pathology, Toxicology and Oncology, Vol.26, 2007, issue 1
Maureen R. Gwinn, Diana L. Whipkey, Lora B. Tennant, Ainsley Weston
Hsp70 Is an Independent Stress Marker Among Frequent Users of Mobile Phones Journal of Environmental Pathology, Toxicology and Oncology, Vol.33, 2014, issue 4
Chinniah Rathika, Ravi Padma Malini, Ramanathan Aravind Selvin Kumar, Muthukalingan Krishnan, Karuppiah Balakrishnan, Vijayan Murali, Thirunavukkarasu Manikandan
Common Variants rs3815188 and rs1043994 on Notch3 Gene Confer Susceptibility to Lung Cancer: A Hospital-Based Case–Control Study Journal of Environmental Pathology, Toxicology and Oncology, Vol.38, 2019, issue 1
Faruk Saydam, Cansu Ozbayer, Guntulu Ak, Irfan Degirmenci, Muzaffer Metintaş, Emine Yagci
Comprehensive Analysis of Prognostic and Immune Infiltrates for RAD51 in Human Breast Cancer Critical Reviews™ in Eukaryotic Gene Expression, Vol.31, 2021, issue 4
Feifei Li, Youyang Shi, Yang Zhang, Sheng Liu
A Signature Constructed Based on the Integrin Family Predicts Prognosis and Correlates with the Tumor Microenvironment of Patients with Lung Adenocarcinoma Journal of Environmental Pathology, Toxicology and Oncology, Vol.42, 2023, issue 2
Yuanyuan Lu, Xuecong Ning, Dengxiang Liu, Zhigang Cai, Zhihua Wang, Xiaochong Zhang, Shusen Zhang, Xiaoling Zhao, Aimin Li, Shubo Chen , Zhiguo Gao, Yang Zhang
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen Preise und Aborichtlinien Begell House Kontakt Language English 中文 Русский Português German French Spain