Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
Journal of Environmental Pathology, Toxicology and Oncology
Impact-faktor: 1.241 5-jähriger Impact-Faktor: 1.349 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i2.10
pages 93-102

Evaluating the Anti-Fertility Potential of α-Chlorohydrin on Testis and Spermatozoa in the Adult Male Wild Indian House Rat (Rattus rattus)

Nithar Ranjan Madhu
Department of Zoology, Bajkul Milani Mahavidyalaya, Bajkul, India
Bhanumati Sarkar
Department of Life Science, Ranaghat Vivekananda Balika Vidyalaya, Ranaghat, West Bengal, India
Surjyo Jyoti Biswas
Department of Zoology, Midnapore College, Midnapore, West Bengal, India
Biplab Kumar Behera
Department of Zoology, Siliguri College, West Bengal, India
Ashis Patra
Department of Life Science, Barda Gangadhar High School, Barda, West Bengal, India

ABSTRAKT

To examine the effects of α-chlorohydrin on testis and cauda epididymis in the male house rat (Rattus rattus), 24 adult male rats were segregated into two groups. Group I rats were force-fed daily by intragastric intubation with α-chlorohydrin at a single dose of 1.0 mg/100 g body weight/d for 5, 15, and 45 days. Another group was fed with distilled water, which served as the control. The treated male rats were paired with 24 adult proestrus female rats for 5 days after the last oral treatment and fertility was tested. At the end of the experiments, all of the male rats were weighed and killed by cervical dislocation. The right testes were removed, weighed, and processed for ultrastructural changes of spermatozoa from the cauda epididymis and testis under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The seminiferous tubular area, nuclear diameter of the Sertoli and Leydig cells, percentage of spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids, spermatozoa, and Sertoli cells in each group were compared morphometrically. Our results showed that the percentages of primary spermatocytes steadily increased from 5 to 15 days, but primary and secondary spermatocytes decreased significantly at 45 days. There was a steady decline in the percentages of spermatozoa and spermatids at all fixation intervals in the treated animals, but the percentages of spermatogonia and Sertoli cells increased significantly at 15 and 45 days. Seminiferous tubular areas, nuclear diameter of Leydig and Sertoli cells, and fertility rates were reduced after 45 days of treatment. SEM and TEM studies revealed severe morphological abnormalities in the spermatozoa, including deglutination of the acrosomal part, loss of head capsules, and fragmentation of tail fibrils. There was an enhanced anti-fertility effect and a lower number of implantation sites in the rats treated for 5 days. Our results validate α-chlorohydrin as a successful anti-fertility agent that prevents spermatogenesis.


Articles with similar content:

Effect of Bioactive Ceramic and Recombinant Human Transforming Growth Factor-Beta (rhTGF-beta) on Regeneration of Parietal Bone Defects in Rat
Journal of Long-Term Effects of Medical Implants, Vol.21, 2011, issue 1
Fariborz Nasseri, Mahdi Kadkhodazadeh, Gholam Ali Gholami
Protective Effect of Troxerutin on Nickel-Induced Testicular Toxicity in Wistar Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 2
Ramalingam Ramakrishnan, Abdulkadhar Mohamed Jalaludeen, Leelavinothan Pari, Perumal Elangovan
ALA and Malignant Glioma: Fluorescence-Guided Resection and Photodynamic Treatment
Journal of Environmental Pathology, Toxicology and Oncology, Vol.26, 2007, issue 2
Tobias Beck, Jorg Ch. Tonn, Friedrich-Wilhelm Kreth, Herbert Stepp, Walter Stummer, Thomas Meinel, Thomas Pongratz
The Experience on PDT by the Multidisciplinary Group in Padova
Forum on Immunopathological Diseases and Therapeutics, Vol.2, 2011, issue 3
Giorgio Battaglia, Lorenzo Norberto, Luigi Corti, Lamberto Toniolo
Cigarette Smoking Induces Apoptosis in Rat Testis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.21, 2002, issue 3
Joseph C. Dunbar, Yang Jiang, Atul Rajpurkar, Haikun Li, C. B. Dhabuwala