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Journal of Environmental Pathology, Toxicology and Oncology
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ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i2.20
pages 103-111

In Vitro 14C-Labeled Amino Acid Uptake Changes and Surface Abnormalities in the Colon after 1,2-Dimethylhydrazine—Induced Experimental Carcinogenesis: Protection by Zinc

Vijayta Dani Chadha
Centre for Nuclear Medicine, Institute for Emerging Ares in Science and Technology, and Department of Biophysics, Panjab University, Chandigarh, India
Devinder K. Dhawan
Department of Biophysics and Centre of Nuclear Medicine, Institute for Emerging Areas in Science and Technology, Panjab University, Chandigarh, India

ABSTRAKT

The present study explored the regulatory role of zinc on the in vitro uptake of 14C-glucose and 14C-labeled amino acids and on colonic surface abnormalities after 1,2-dimethylhydrazine (DMH)–induced colon carcinogenesis. Rats were segregated into four groups: control, DMH-treated, zinc-treated, and DMH + zinc−treated. Colon carcinogenesis was induced through weekly subcutaneous injections of DMH (30 mg/kg body weight) for 16 weeks. Zinc (in the form of zinc sulfate) was given to rats at a dose level of 227 mg/L in their drinking water. DMH treatment caused a significant decrease in the activities of disaccharidases (sucrase, lactase, and maltase), but a significant increase in the activity of alkaline phosphatase. In vitro uptake of 14C-D-glucose and the amino acids 14C-glycine, 14C-alanine, 14C-lysine, and 14C-leucine were significantly higher in the colons of DMH-treated rats. Zinc supplementation of DMH-treated rats resulted in regulating the altered intestinal enzyme activities and in vitro uptake of 14C-amino acids and 14C-glucose. Scanning electron microscopy revealed drastic alterations in the colon surface morphology after DMH treatment, which were restored after zinc supplementation. Our results confirm a beneficial effect of zinc against DMH-induced alterations in the colons of rats.


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