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ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537
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Electroporation Enhances Radiation and Doxorubicin-Induced Toxicity in Solid Tumor In Vivo
ABSTRAKT
Treatment of cancer patients is subject to limitations in radiotherapy and chemotherapy. This necessitates development of new protocols, and the present work reports on the effects of a combination of local electroporation with ionizing radiation and/or anticancer drug doxorubicin hydrochloride (DOX) on subcutaneous solid tumor murine fibrosarcoma. Localized treatment of fibrosarcoma tumor, grown in right hind leg of Swiss mice, has been carried out using DOX (0.6 mg/kg body weight), radiation (Co 60 γ-rays, dose rate 0.37 Gy/min) and electroporation (1 kV/cm, 200 μs, 8 pulses per burst, 10 bursts) individually or in combinations. Measurements of the tumor growth kinetics after treatment with combinations have revealed significant growth delay. The treatment groups, (i) radiation and electroporation, (ii) DOX and electroporation, and (iii) radiation, DOX and electroporation, have yielded tumor growth delays (TGDs) of 1.22, 1.5, and 1.73 days, respectively, compared to control with the tumor volumes being 53%, 57%, and 49% that of control on the final day of observation. These results suggest that the antitumor effects of a moderate dose of γ radiation and low concentration of DOX can be significantly enhanced by combination with electroporation.
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Meschini Stefania, Condello Maria, Lista Pasquale, Vincenzi Bruno, Baldi Alfonso, Citro Gennaro, Arancia Giuseppe, Spugnini Enrico P., Electroporation adopting trains of biphasic pulses enhances in vitro and in vivo the cytotoxic effect of doxorubicin on multidrug resistant colon adenocarcinoma cells (LoVo), European Journal of Cancer, 48, 14, 2012. Crossref
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Kulbacka Julita, Daczewska Małgorzata, Dubińska-Magiera Magda, Choromańska Anna, Rembiałkowska Nina, Surowiak Paweł, Kulbacki Marek, Kotulska Małgorzata, Saczko Jolanta, Doxorubicin delivery enhanced by electroporation to gastrointestinal adenocarcinoma cells with P-gp overexpression, Bioelectrochemistry, 100, 2014. Crossref
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Kranjc Simona, Tevz Gregor, Kamensek Urska, Vidic Suzana, Cemazar Maja, Sersa Gregor, Radiosensitizing Effect of Electrochemotherapy in a Fractionated Radiation Regimen in Radiosensitive Murine Sarcoma and Radioresistant Adenocarcinoma Tumor Model, Radiation Research, 172, 6, 2009. Crossref
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Shil Pratip, Bidaye Salil, Vidyasagar Pandit B, Analysing the effects of surface distribution of pores in cell electroporation for a cell membrane containing cholesterol, Journal of Physics D: Applied Physics, 41, 5, 2008. Crossref
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Taveira Stephânia F., Nomizo Auro, Lopez Renata F.V., Effect of the iontophoresis of a chitosan gel on doxorubicin skin penetration and cytotoxicity, Journal of Controlled Release, 134, 1, 2009. Crossref
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Venslauskas Mindaugas S., Šatkauskas Saulius, Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation, European Biophysics Journal, 44, 5, 2015. Crossref
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Kranjc Simona, Kamensek Urska, Cemazar Maja, Sersa Gregor, Combined Treatment of Electrochemotherapy with Irradiation, in Handbook of Electroporation, 2016. Crossref
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Mishra K, Membrane Electroporation and Emerging Biomedical Applications, in Handbook of Membrane Separations, 2008. Crossref
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Kumar Binod, Kumar Amit, Pandey B. N., Hazra Banasri, Mishra K. P., Increased cytotoxicity by the combination of radiation and diospyrin diethylether in fibrosarcoma in culture and in tumor, International Journal of Radiation Biology, 84, 5, 2008. Crossref
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Kranjc Simona, Kamensek Urska, Čemažar Maja, Serša Gregor, Combined Treatment of Electrochemotherapy with Irradiation, in Handbook of Electroporation, 2017. Crossref
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Dos Anjos Denner S., Bueno Cynthia, Magalhães Larissa F., Magalhães Georgia M., Mattos-Junior Ewaldo, Pinto Marcela M. R., De Nardi Andrigo B., Brunner Carlos H. M., Leis-Filho Antonio F., Calazans Sabryna G., Fonseca-Alves Carlos E., Electrochemotherapy induces tumor regression and decreases the proliferative index in canine cutaneous squamous cell carcinoma, Scientific Reports, 9, 1, 2019. Crossref
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Rembiałkowska Nina, Dubińska-Magiera Magda, Sikora Andrzej, Szlasa Wojciech, Szewczyk Anna, Czapor-Irzabek Hanna, Daczewska Małgorzata, Saczko Jolanta, Kulbacka Julita, Doxorubicin Assisted by Microsecond Electroporation Promotes Irreparable Morphological Alternations in Sensitive and Resistant Human Breast Adenocarcinoma Cells, Applied Sciences, 10, 8, 2020. Crossref